FastQCFastQC Report
Wed 6 Sept 2023
SRR13723997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13723997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59528521
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length48
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA20250773.4018600932484113No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAA14267812.396802366381654No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA10764491.8082911886892001No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA9001821.5121860662387363No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA6532551.0973815391785058No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA6322101.0620287374517503No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA5920460.9945585579053778No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCAAAAAAAAAAAAAAAAAAA5388800.9052467471852694No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAA3592250.6034502352242214No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAA3166520.531933255993375No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAA2850430.47883433892133825No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA2800500.47044676282147174No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAAAAAAAAAAAAAAAA2729260.45847939007253347No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAAA2499390.4198642865660983No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAA2497470.41954175209560474No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2371440.3983703878683631No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA2157700.36246490988748065No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2061790.34635330516610685No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAAAAAAAAAAAAAAAAAAA1843880.3097473226321212No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGAAAAAAAAAAAAAAAAAA1825190.3066076511459104No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAA1442200.2422704236176135No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA1366290.22951855296388096No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCAAAAAAAAAAAAAAAAAAAA1341350.22532896458153226No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAA1275630.214288878435263No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAA1271210.21354637720631428No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAA1247200.20951301645811088No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAAAAAAAAAAAAAAAAA1229120.20647581686096317No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAAAAAAAAAAAAAA1218380.20467163966663982No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAA1215760.2042315145037788No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAA1205740.2025482877358905No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAAAAAAAAAAAAAAAAAA1173850.19719119176503647No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAA1172000.19688041636377962No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGAAAAAAAAAAAAAAAAAAAAA1161140.19505608076505043No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAAAAAAAAAAAAAAA1146720.19263371250228103No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAA1138390.19123438326310846No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGAAAAAAAAAAAAAAAAAAAAT1067840.1793829213395038No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAA1046350.17577288708382324No Hit
ATGGTAATCCTGCTCAGTACGAGAGGAACCGCAAAAAAAAAAAAAAAA1019960.17133971798157055No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCAAAAAAAAAAAAAAAAAAAA1017330.17089791295167572No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAAAAAAAA920610.15465023900056243No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAA905530.15211699951356092No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAA836240.14047720083621765No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAA822900.1382362582131009No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCAAAAAAAAAAAAAAAA777980.13069029549717856No Hit
AATTATTCCCCATGAACGAGGAATTCCCAAAAAAAAAAAAAAAAAAAA765890.1286593362532894No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAA760270.12771525098028222No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAA759350.1275607032131707No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGAAAAAAAAAAAAAAAAAAATC726460.1220356205389346No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA694090.11659789095045717No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAAA693630.11652061706690144No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA660830.1110106531959697No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA660600.11097201625419183No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA657910.11052013202209408No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAT651870.10950549233366641No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA647510.1087730703069206No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAA639100.10736030213147744No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAAAAAAAAAAAAAAA634130.10652540821566858No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA629850.10580642512519337No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAAAAAAAAAAAAAAAA628090.10551076852724091No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAAAAAAAAAA613700.103093439865573No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAAAAAAAAAAAAAAAA613210.1030111263809158No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAA610470.10255084281364894No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAAAA604330.10151940445488307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)