FastQCFastQC Report
Wed 6 Sept 2023
SRR13723999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13723999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66055854
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length48
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAA10867221.6451562339955519No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA6465810.9788398163772132No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA4931360.7465439777676631No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA4191500.6345387647247737No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAAAAAAAAAAAAAAAA4178170.632520775524301No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA3713350.562153053081412No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCAAAAAAAAAAAAAAAAAAA3599470.5449130973312373No Hit
ATGGTAATCCTGCTCAGTACGAGAGGAACCGCAAAAAAAAAAAAAAAA2872730.43489408221109366No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAAAAAAAAAAAAAAA2822420.4272778003899549No Hit
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAAAATC2738340.4145491783362607No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAA2475520.3747616373258909No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAAAAAAAAAAAAAAAAA2343800.35482093683929966No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2292660.3470790037776213No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA2075670.3142295306635503No Hit
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAAAAAA1971990.298533722688681No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA1918660.29046025201642234No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGAAAAAAAAAAAAAAAAAA1886720.28562495006120125No Hit
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAAATCG1767840.2676280591270533No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCAAAAAAAAAAAAAA1767130.2675205743309291No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1765270.26723899444249105No Hit
AATTATTCCCCATGAACGAGGAATTCCCAAAAAAAAAAAAAAAAAAAA1689600.25578353736823994No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAAAAAAAAAAAAAAAAAAA1527940.23131030900001687No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAAAAAAAAAAAAAAAAAA1508810.2284142749861352No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGAAAAAAAAAAAAAAAAAAAAA1428470.21625184045005308No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAAAAAAAAAAAAAAAAAAA1301980.19710289416589782No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGAAAAAAAAAAAAAAAAAAAAT1270210.19229332800693183No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAA1149390.17400274622140227No Hit
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAAAAAT1129510.17099317192992464No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAAAAAAAAAAAAAAAAA1123780.1701257242090913No Hit
AAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAAAATCGTATGCCG1089770.16497705108770525No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAA1001620.15163228379425692No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA964440.14600371376623184No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA962020.14563735713718878No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAAAAAAAAAAAAAAA929970.14078540260792027No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCAAAAAAAAAAAAAAAAAAAA919490.13919886646231233No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTAAAAAAAAAAAAAAAAA918170.13899903557374338No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA904320.1369023251141375No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAA899620.13619080604120265No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCAAAAAAAAAAAAAAAAA872320.1320579399367087No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAAAAAAAAAAAAA856040.12959335897769184No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGAAAAAAAAAAAAAAAAAAATC855310.12948284644083172No Hit
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAATCGT810750.12273704008126214No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAA808950.12246454341503175No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGACCAAAAAAAAAAAAAAAAA807250.12220718545248087No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAA787770.1192581659757211No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAA785010.11884033775416787No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAAAAAAAAAAAAAAAA774090.11718719131237028No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAAAAAAAAAAAAT773080.11703429040520769No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCAAAAAAAAAAAAAAAAAA764530.11573993124061344No Hit
AAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAAATCGTATGCCGT761580.11529333948206923No Hit
AAAAAGCTCGCTTGATCTTGATTTTCAAAAAAAAAAAAAAAAAAAATC756580.11453640429809597No Hit
AAGGTGAACAGCCTCTGGCATGTTGGAACAAAAAAAAAAAAAAAAAAA731840.11079108900779636No Hit
GGAACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAAAAAAAAAA721500.10922574704733967No Hit
ACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAAAATCGTATGC713450.10800708140114273No Hit
GGAACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAAAAAAAATC711990.10778605632742254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)