FastQCFastQC Report
Wed 6 Sept 2023
SRR13762817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14072000
Total Bases656.2 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT6510004.626208072768619No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT4167912.961846219442865No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3356172.3849985787379193No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT3292982.340093803297328No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT3049072.1667637862421834No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT2787481.9808698123934054No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT2604161.8505969300739056No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC2599371.847193007390563No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2427871.7253197839681638No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT2309551.6412379192723137No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA2238131.590484650369528No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT1937831.377082148948266No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1931031.372249857873792No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1915331.3610929505400797No Hit
CGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1908211.356033257532689No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1504021.0688032973280273No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1467841.0430926662876634No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1309190.9303510517339398No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1135370.8068291642978965No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1108880.7880045480386584No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT993540.7060403638430927No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT953060.6772740193291643No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT919570.6534749857873792No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT832760.591785105173394No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT778420.5531694144400228No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT771730.5484152927799887No Hit
TCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT716500.5091671404206936No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT715600.5085275724843661No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT704020.5002984650369529No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC699060.49677373507674816No Hit
TGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT655150.4655699260943718No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT630240.4478681068789085No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT591340.4202245594087549No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT510180.3625497441728255No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA509720.3622228538942581No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT463810.3295977828311541No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT459960.3268618533257533No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT454810.3232021034678795No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT448000.3183627060830017No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT443600.3152359295054008No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT428830.3047399090392268No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT428450.3044698692438886No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT424290.301513644115975No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT399130.2836341671404207No Hit
GCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT379600.26975554292211484No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT375630.2669343376918704No Hit
TCCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT370110.263011654349062No Hit
TCTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT367510.26116401364411596No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT355490.2526222285389426No Hit
TCTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT342500.24339113132461626No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT342300.2432490051165435No Hit
TTGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT333280.23683911313246162No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT330390.23478538942581012No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT329730.23431637293917No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT324040.2302728823194997No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT322700.22932063672541214No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT320670.22787805571347358No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT315390.22412592382035249No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT314920.22379192723138147No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT312510.22207930642410462No Hit
CCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT305430.2170480386583286No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT296510.21070920977828309No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT290310.2063032973280273No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT286670.2037166003411029No Hit
GCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT269860.1917708925525867No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT267100.1898095508811825No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT262010.18619243888573053No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT259780.18460773166571914No Hit
ACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT256110.18199971574758386No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACC253160.1799033541785105No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT239620.1702814098919841No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT236300.16792211483797612No Hit
ATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATCAA232670.16534252416145537No Hit
CCCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT229350.16298322910744742No Hit
GCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT228480.16236498010233086No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT213890.1519968732234224No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT211570.15034820920977826No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT208570.14821631608868674No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA205130.1457717453098351No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA202030.14356878908470722No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT200860.1427373507674815No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT197580.14040648095508812No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAAT195580.13898521887436044No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAAT194780.13841671404206934No Hit
GCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT194410.13815378055713473No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN191770.1362777146105742No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT190000.13501989766913017No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT186380.13244741330301307No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT183710.1305500284252416No Hit
CTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT177130.12587407617964752No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCA173200.12308129619101763No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT167340.1189169982944855No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT165820.11783683911313246No Hit
AGGCCGAAGTGGAGAAGGGTTCCATGTGCTGTAGGCACCATCAAT160240.11387151790790222No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT156450.11117822626492327No Hit
CGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT153120.10881182490051165No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT152600.10844229675952245No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAAT152230.10817936327458784No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT149980.1065804434337692No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT143520.10198976691301875No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT141390.10047612279704378No Hit
TGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT140980.1001847640704946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)