FastQCFastQC Report
Wed 6 Sept 2023
SRR13762818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13458704
Total Bases627.7 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT6345164.714540122139546No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT3986482.962008823435005No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3224442.395802745940471No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT3174232.3584960334962415No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2915872.1665310419190438No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT2697982.0046358104019526No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT2462131.8293960547761507No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC2461501.8289279562133176No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2321691.725047225943895No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT2224251.6526479815589972No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA2157241.6028586407725438No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT1879661.3966129279609687No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1853331.3770493800889No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1830891.3603761550889297No Hit
CGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1825611.356453043324231No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1440801.0705339830640455No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1401501.0413335489063436No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1261770.9375122597242648No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1096580.8147738444949825No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1070360.7952920281180119No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT948330.704622079510776No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT946690.7034035372202257No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT883780.6566605521601485No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT797310.5924121668772863No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT753890.5601505167213723No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT733900.5452976750213097No Hit
TCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT686800.5103017348475752No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT677640.5034957303466961No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT674040.5008208814162196No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC658820.48951221454903826No Hit
TGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT629640.4678310779403425No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT628610.4670657739407895No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT583270.4333775376886215No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT485860.3610005837114777No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA481260.3575827211892022No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT446790.3319710426798895No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT441240.3278473172454049No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT433830.3223415865301741No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT428740.31855964734791703No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT424090.3151046341460515No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT418550.31098833884748484No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT413710.3073921530631775No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT407980.30313468518216913No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT379690.2821148306701745No Hit
GCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT364960.2711702404629748No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT358960.26671215891218053No Hit
TCCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT353820.26289306905033355No Hit
TCTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT350600.26050056528474064No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT343550.25526231946255745No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT339670.2523794267263772No Hit
TCTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT326510.24260136785830194No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT322650.2397333353939577No Hit
TTGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT318160.23639720436678005No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT314790.2338932485624173No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT314460.2336480540771236No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT304990.2266117153627868No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT304470.2262253482950513No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT302760.22495479505307495No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT298160.22153693253079942No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT297470.22102425315245808No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT291340.21646957983473No Hit
CCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT289380.2150132731948039No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT285000.21175887366272414No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT270490.20097774644572017No Hit
GCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT256950.1909173424127613No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT254420.18903751802550975No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT251680.1870016607839804No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT250290.18596887189137973No Hit
ACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT248200.18441597348451977No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACC238930.17752823748854274No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT229970.1708708357060234No Hit
ATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATCAA222690.16546169675772646No Hit
CCCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT219610.16317321489498543No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT218980.16270511633215204No Hit
GCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT212910.15819502382993192No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT211050.15681301854918572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206580.15349174779384403No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT202680.15059399478582783No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT200210.14875875121408422No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA191840.14253972745072632No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAAT191790.14250257677113637No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA191420.14222766174217072No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAAT191200.14206419875197493No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT189160.1405484510247049No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT186760.13876521840438721No Hit
GCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT184370.13698941591998753No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN182520.13561484077515934No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT182140.1353324956102757No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT179710.13352697258220406No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT173730.12908375130324584No Hit
CTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT170650.12679526944050482No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCA167320.12432103417981404No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT158130.11749273927118094No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT157120.11674229554346392No Hit
AGGCCGAAGTGGAGAAGGGTTCCATGTGCTGTAGGCACCATCAAT155340.11541973135006163No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT149260.11090220871192354No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT146390.10876975970346031No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT144790.10758093795658186No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAAT144330.1072391517043543No Hit
CGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT141130.10486150821059738No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT138510.10291481260008392No Hit
TGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT138140.10263989757111829No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT137430.10211235792094096No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)