FastQCFastQC Report
Wed 6 Sept 2023
SRR13762820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13241223
Total Bases619.8 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT6107944.612821640417959No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT3708162.8004663919639445No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT3058822.3100736238638984No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3005992.2701754966289744No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2711552.0478093299992004No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT2610011.9711245705929126No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC2296651.7344696936227113No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT2278901.7210645874629555No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT2135051.612426586275301No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2133441.6112106865053175No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA2017971.5240057508283034No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT1804221.3625780639749063No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1786671.349324001264838No Hit
CGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1738581.313005603787505No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1713321.2939288160919877No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1376221.0393450816438934No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1347131.0173758118868625No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1223670.9241366903948374No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1065810.8049180955565812No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1035340.7819066259967073No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT884940.6683219518317908No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT867780.6553624238486128No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT851980.643429991323309No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT770110.5816003551937763No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT690050.5211376622839144No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT676550.5109422294300157No Hit
TCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT660310.4986775013153997No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT625680.47252432800202815No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT625610.4724714627946376No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC612900.462872651567004No Hit
TGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT608800.4597762608484126No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT575240.43443116999086867No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT534650.40377690187681303No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT465450.35151586828497644No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA438680.33129870254431937No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT419150.31654930968234585No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT413940.3126146278179893No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT412410.3114591454278808No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT403010.3043601032925735No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT402920.3042921337402142No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT400530.30248716451644986No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT399430.30165642554316924No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT389570.29420998347358096No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT363610.2746045437041579No Hit
GCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT347000.26206038520762015No Hit
TCCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT344480.2601572377415591No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT342330.25853352065741964No Hit
TCTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT341190.2576725729942015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG336580.2541910214789072No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT318160.24027991976269866No Hit
TCTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT315790.23849005488390307No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT310780.23470641646923399No Hit
TTGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT308080.23266732989845423No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT304690.230107143426253No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT302870.22873264803409776No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT294760.22260783614927415No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT284840.2151160810447796No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT284240.21466295069571747No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT280460.21180822949662578No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT279110.21078868621123592No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT277950.20991263420304906No Hit
CCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT277050.2092329386794558No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT263300.1988487015134478No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT260850.1969984192547773No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT255150.1926936809386867No Hit
GCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT244770.18485452589991122No Hit
ACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT237290.17920550088160286No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT236780.17882034008490003No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT236630.17870705749763446No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT234540.17712865344840126No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACC220460.16649519459040904No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT213930.1615636259581158No Hit
ATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATCAA207110.15641304432377584No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT205920.15551433579813587No Hit
GCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT205760.15539350103838595No Hit
CCCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT204620.15453255337516786No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT197230.1489514979092188No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT195040.14729757213514189No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT192710.1455379159462838No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAAT182520.13784225218471133No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAAT181140.13680005238186835No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT179880.1358484786488378No Hit
GCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT178490.13479872667351045No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN178240.1346099223614012No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA177100.1337489746981831No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA176950.13363569211091753No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT175380.13245000103087154No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT170840.1290213147229678No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT166720.1259098196594076No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT165990.12535851106804863No Hit
CTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT162850.1229871289079566No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCA154490.11667351271102373No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT150170.11341097419777614No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT147810.11162866149146496No Hit
AGGCCGAAGTGGAGAAGGGTTCCATGTGCTGTAGGCACCATCAAT146720.11080547469066868No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT144790.10934790540118536No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT141950.10720308841562444No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAAT136000.10270954578742461No Hit
CGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT134230.10137281125769122No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT134180.10133505039526937No Hit
TGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT132770.10027019407497328No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)