FastQCFastQC Report
Wed 6 Sept 2023
SRR13762821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13012779
Total Bases601.8 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT6232394.78943813615831No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT4038433.1034339398217705No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT3386162.60218051808918No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2512041.9304408381945162No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT2492211.9152019718462907No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2383811.8318992430440877No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2225691.7103879194444167No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT2222671.7080671238633962No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC2217291.7039327264376043No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT2092081.6077119268681963No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1848141.4202500480489217No Hit
CGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1567141.2043084724638757No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT1509491.1600058680778333No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1460891.1226579656812739No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1348741.036473454286744No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1336601.0271441634411835No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1291400.9924090772616672No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1099140.8446620049414502No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1094780.8413114523807712No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1018940.7830302812335475No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT938700.7213678185113265No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT886110.6809536994365308No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT864270.6641701976188176No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT851050.654010953386667No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT801730.6161097487323807No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT753400.5789693346824687No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT748620.5752960224714491No Hit
TGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT731710.5623011041684486No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT622150.4781069439510192No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT604160.4642820722614286No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT581770.44707590899684074No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT562670.4323980296599212No Hit
TCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT532030.4088519446922137No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT528080.4058164670282958No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN523510.4023045346424465No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT522480.4015130050237539No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT488880.3756922329965029No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT482890.371089065602359No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT453500.34850357483209393No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT443120.3405268006165324No Hit
GCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT435540.3347017574032418No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT431510.3316048017106876No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT405010.31124020472490926No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA371590.2855577582620899No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT369380.2838594277210118No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT361330.2776732010894829No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT360630.27713526833891516No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC351800.2703496309281822No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT345220.26529306307284556No Hit
TCTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT331390.25466504887234315No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT326450.2508687806040509No Hit
TTGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT323650.24871704960177993No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT302830.2327173926491797No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT297230.22841393064463783No Hit
GCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT289370.2223737143311202No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT288990.22208169369509773No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT285340.21927675863856597No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT276270.21230668714192408No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT273540.21020874941470993No Hit
TCCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT267730.2057439075849978No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT267570.20562095152772517No Hit
CCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT265340.20390725147948796No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT263350.20237798551715971No Hit
ACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT254790.1957998364530743No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT254290.19541559877409737No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT252820.19428593999790514No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT249480.19171923230233912No Hit
CCCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT234140.17993082031132626No Hit
TCTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT230090.176818495111613No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT226640.17416725512667203No Hit
GCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT225060.17295306406110486No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT209470.16097253323060357No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT205600.15799853359532195No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT203070.15605429093969858No Hit
TGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT190920.14671731534055868No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCA189620.14571829737521863No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT189090.14531100543550304No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACC188440.144811496452833No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA185010.14217562597505115No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT183270.14083847885221135No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT182790.1404696106803935No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT182540.14027749184090502No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT181820.1397241895831782No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT181500.13947827746863295No Hit
ATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATCAA172140.13228534811818446No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAAT169320.13011824760875446No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT166140.12767449597046102No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT165370.12708276994483653No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATNNN164100.12610680624023507No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT162230.12466975732086129No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT153290.1177995876207534No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAAT151710.11658539655518625No Hit
AGGCCGAAGTGGAGAAGGGTTCCATGTGCTGTAGGCACCATCAAT150990.11603209429745945No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT150400.11557869383626665No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT149610.11497159830348305No Hit
GCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT149110.1145873606245061No Hit
CTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT145450.11177474081439484No Hit
TCAGGTTAGTTTTACCCTACTGATGATGCTGTAGGCACCATCAAT143780.11049138696661182No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT141880.10903128378649941No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAAT140820.10821669990706828No Hit
TCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT134610.10344446793417454No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132120.10153096429286934No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT130980.10065490238480189No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)