FastQCFastQC Report
Wed 6 Sept 2023
SRR13762822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12452748
Total Bases576.1 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT6071214.875397783685979No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT3862803.101965927520576No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT3305132.65413706275916No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT2426131.9482687676647756No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2395471.9236476960747941No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2299461.8465482478244963No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2123391.705157769192792No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT2103811.6894343320847736No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC2080791.670948452502211No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT2014551.6177553741551665No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1745891.4020118290356476No Hit
CGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1504471.2081429737436267No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT1467721.1786314153309776No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1416721.137676599574648No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1292311.0377709401972963No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1286371.0330009087150884No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1230150.9878542471107582No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1083790.8703219562461234No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1058090.8496839412473456No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT987820.7932546294199481No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT930930.7475699339615641No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT863680.6935657896554238No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT832080.6681898645985609No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT809310.6499047439167644No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT760970.6110860028645886No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT744550.5979001582622567No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT724880.5821044479499625No Hit
TGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT698400.5608400651807939No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT610810.49050217670830565No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT578880.46486124990243116No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT550230.44185427987461084No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT537160.43135860454254754No Hit
TCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT511770.41096953058072No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT506180.40648056155958506No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT501930.4030676602465576No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN497840.3997832446300206No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT478790.384485416391627No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT469000.37662369783761784No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT435300.3495613980143178No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT419940.337226771151235No Hit
GCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT418930.33641570519213915No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT414340.3327297717740694No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT381350.30623762722894576No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA350880.28176913240354656No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT347190.2788059310282357No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT346930.27859714177143874No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT346660.2783803221586111No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC337850.27130557849560594No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT337380.2709281517621653No Hit
TCTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT316430.25410455587794756No Hit
TTGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT310970.24971998148521113No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT309090.2482102745514484No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT300990.2417056861666196No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT279910.2247776956540034No Hit
GCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT279360.22433602607231756No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT275030.22085888191104483No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT272060.21847386616994097No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT265560.21325413475001662No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT263390.21151154749136494No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT263130.21130275823456798No Hit
TCCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT254680.20451710738866635No Hit
CCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT253180.20331255398406842No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT248460.1995222259376003No Hit
ACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT245400.1970649369922205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG244660.1964706906459522No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT243870.1958362925195306No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT238940.1918773269964188No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT237710.1908895932046485No Hit
TCTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT220490.1770613201198643No Hit
CCCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT220310.17691677371131256No Hit
GCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT214840.17252416896254547No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT214270.17206643866879825No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT199170.15994060106251248No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT198300.15924196008784566No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT196960.15816589237973822No Hit
TGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT184010.14776658132004278No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACC181450.14571081017619564No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCA181230.1455341423435213No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT180710.14511656382992733No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT177510.14254684990011843No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT176590.14180805714529837No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA175390.14084441442162005No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT174710.14029835021153564No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT172050.13816227550738197No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT171480.1377045452136348No Hit
ATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATCAA166630.13380982253876814No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT164990.13249284414974108No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAAT163310.1311437443365914No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT158210.12704826276095846No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATNNN157920.12681538243606955No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT152400.12238262590714916No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT148120.11894563352602976No Hit
GCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT146180.1173877444560831No Hit
AGGCCGAAGTGGAGAAGGGTTCCATGTGCTGTAGGCACCATCAAT144590.1161109178472093No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT144180.11578167324995253No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT142290.11426393596015916No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAAT141120.11332438430457278No Hit
CTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT138750.11142118992530806No Hit
TCAGGTTAGTTTTACCCTACTGATGATGCTGTAGGCACCATCAAT137320.1102728490129247No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT135650.10893177955580567No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAAT134300.10784768149166754No Hit
TCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT129580.10405735344519941No Hit
CGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT125200.10054005750377347No Hit
AGGTTAGTTTTACCCTACTGATGATGCTGTAGGCACCATCAAT125010.10038748073919106No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)