FastQCFastQC Report
Wed 6 Sept 2023
SRR13762823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12622802
Total Bases586.3 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT5995284.7495635279710475No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT3681472.9165236054562214No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT3268912.589686505420904No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT2402361.903190749565746No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2288391.8129017630158502No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2224241.7620810339891255No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2012721.5945112661990577No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT2004041.5876348214920903No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT2001711.585788955574206No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC1995891.5811782518651563No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1708011.3531147838649453No Hit
CGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1485461.1768068611073834No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT1422541.1269605591531897No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1378821.0923248261360672No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1284251.0174048519496701No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1284041.0172384863519208No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1238110.980851953472771No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1046590.829126528325486No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT986700.7816806442816737No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT986650.7816410334250669No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT843610.6683222948438865No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT840430.6658030443636841No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT811040.6425197828501152No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT803490.6365385435024649No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT723680.5733116941864413No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT721940.5719332363765193No Hit
TGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT701680.5558829172793806No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT674780.5345722764248382No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT556840.44113818786034986No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT554040.4389199798903603No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT550900.4364324180954435No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT518470.4107408165001717No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN506640.4013688878269658No Hit
TCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT498400.3948410186581395No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT493620.3910542207665144No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT488460.38696638036467657No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT462550.36644003447095186No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT437220.34637317451386784No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT436180.34554926869644315No Hit
GCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT416280.3297841477668746No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT411540.3260290385605351No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT408520.32363654282147497No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT377420.29899859001194823No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG358730.284192051812268No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT335700.26594729125910394No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT334520.2650124750431798No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT333300.26404597014197007No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT332380.26331713038040205No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA327170.25918967912195723No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC326630.2587618818706021No Hit
TCTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT321800.25493547312237014No Hit
TTGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT308150.2441217092686711No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT304000.2408340081702937No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT283000.22419744839537212No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT275130.21796269956543718No Hit
GCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT274670.21759827968465323No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT265160.21006429475801014No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT259370.20547735756292462No Hit
TCCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT255400.2023322555483323No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT252360.19992391546662935No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT247480.19605789586179043No Hit
CCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT247200.19583607506479145No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT246780.19550334386929305No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT242000.19171654597766802No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT241560.19136797043952683No Hit
ACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT241120.19101939490138561No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT233280.1848084125854149No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT227750.1804274518446855No Hit
TCTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT221100.17515920791596032No Hit
CCCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT217640.17241813663875896No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT215160.17045343815105396No Hit
GCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT214740.1701207069555555No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT194260.15389610008934626No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT188250.1491348751251901No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT188210.14910318643990456No Hit
TGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT187510.14854863444740715No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT177610.14070568483922982No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT175100.13871721983756063No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT174540.13827357824356273No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCA170280.13489873326065005No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT170190.13482743371875755No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT169570.13433625909683128No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACC168240.13328261031108624No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA166610.13199129638569945No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT163320.12938490202096176No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT161350.12782423427064765No Hit
ATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATCAA159970.1267309746282957No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAAT157650.12489303088173292No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATNNN149350.11831762868497818No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT147030.11647968493841543No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT146660.11618656459952395No Hit
GCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT144400.11439615388088953No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT142260.11270080921811179No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAAT141390.11201158031315075No Hit
AGGCCGAAGTGGAGAAGGGTTCCATGTGCTGTAGGCACCATCAAT141380.11200365814182937No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT139630.11061727816058589No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT138990.11011025919601687No Hit
CTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT137200.10869219052948784No Hit
TCAGGTTAGTTTTACCCTACTGATGATGCTGTAGGCACCATCAAT135420.10728204403428018No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT132830.10523020166203986No Hit
TCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT129420.10252874124144543No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAAT128810.10204548879084058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)