FastQCFastQC Report
Wed 6 Sept 2023
SRR13762824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12237998
Total Bases568.5 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT5837234.7697589099132065No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT3578622.9241874365398655No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT3173652.5932754687490553No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT2332011.905548603619644No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2230421.822536659999454No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2119901.732227771241669No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1962661.6037427036677079No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC1946911.5908729516053197No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT1943971.5884705978870075No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1942461.5872367359432482No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1635101.3360845458546406No Hit
CGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1428511.167274255151864No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT1399601.143651110255125No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1318031.0769980514786814No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1247201.0191209379181139No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1232821.0073706500033748No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1182740.9664489240805563No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1023640.8364440000725609No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT983860.8039386834349866No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT957680.7825462955623952No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT851490.6957755672128726No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT809730.6616523388874553No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT805380.658097835936891No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT777650.6354389010359374No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT701060.5728551352925535No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT695610.5684017925154098No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT682800.5579343941713343No Hit
TGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT673110.5500164324262841No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT557580.4556137368219867No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT534300.43659101758310465No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT527600.4311162659121206No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT502910.4109413974409866No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN491510.401626148329163No Hit
TCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT486370.3974261149576916No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT476620.38945912558573714No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT466480.3811734566389045No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT448990.3668819033962908No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG447740.3658604945024505No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT440240.35973204113940854No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT416480.3403171008852919No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT397230.324587403920151No Hit
GCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT396490.32398272985499754No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT391280.3197254975854711No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT363290.29685410963459874No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT328260.2682301467936177No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT327890.267927809761041No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT323370.2642343952009144No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA321410.26263282605537275No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT321380.2626083122419206No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC309070.2525494774553812No Hit
TCTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT306220.25022066517742525No Hit
TTGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT305370.24952610712961387No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT295410.2413875210634942No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT276140.22564148155605188No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT267000.2181729397242915No Hit
GCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT261310.21352348643953037No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT255220.20854718230874036No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT254810.20821216019156075No Hit
TCCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT247030.20185491123629862No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT245320.20045762386952506No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT243280.19879068455477766No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT241750.1975404800687171No Hit
CCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT241310.19718094413808535No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT238740.19508092745234965No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT234310.19146105433257957No Hit
ACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT230890.18866647959903246No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT224680.18359212021443377No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT219640.17947379955446963No Hit
TCTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT210660.1721359980611208No Hit
CCCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT208000.1699624399350286No Hit
GCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT207860.16984804213891846No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT204210.16686552816890476No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT184800.15100509086535233No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT182620.14922375375449481No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT182160.14884787528156157No Hit
TGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT179200.1464291790209477No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT168850.1379719133799499No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT168470.13766140507622243No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCA165650.13535710661171868No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT165420.13516916737525206No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT164950.13478511763116813No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACC163450.1335594269585597No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT161960.13234190755710207No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA159850.1306177693442996No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT156590.12795393494916407No Hit
ATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATCAA155000.12665470283619917No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAAT152830.1248815369964924No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT152070.12426052038903748No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATNNN150230.12275700649730455No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAAT146450.11966826600233144No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT143960.11763361948580152No Hit
GCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT139720.11416900051789515No Hit
AGGCCGAAGTGGAGAAGGGTTCCATGTGCTGTAGGCACCATCAAT138970.11355615518159098No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT136360.1114234534112524No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAAT135230.11050009977122074No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAAT135080.1103775307039599No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT133220.10885767426992551No Hit
CTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT132960.10864522122000674No Hit
TCAGGTTAGTTTTACCCTACTGATGATGCTGTAGGCACCATCAAT132040.10789346427414026No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT129800.10606309953637841No Hit
TCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT125270.1023615137051011No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAAT125220.10232065734934749No Hit

[FAIL]Adapter Content

Adapter graph