FastQCFastQC Report
Wed 6 Sept 2023
SRR13762833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16330533
Total Bases769.8 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT8149824.990541337505641No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT5317403.2561092770211477No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT5200883.1847582684533324No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA4406772.6984851014966873No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT4268862.6140359288946664No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC3220831.9722748792093927No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT3084031.888505414979413No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT2768921.6955478428046407No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT2063851.2637983095836491No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1708001.0458936031052997No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1692171.0362001044301494No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1684531.0315217513108728No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1453970.8903383618893517No Hit
CGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1440130.8818634394847982No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1412050.8646686547218023No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1408260.8623478486587057No Hit
TGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT1344750.823457507480007No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1299430.7957058107044026No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1250490.7657374073461043No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1229620.7529576652519547No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1153710.7064741855027021No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1104670.6764445471559317No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT1099630.6733583037369325No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT1091870.6686064686314892No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1065550.6524894196656043No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT980740.6005560259423254No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT912450.558738652314655No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT893890.5473734384542134No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT866030.5303133706658564No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT849240.5200320161013728No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN826830.5063092551847512No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACC816370.49990407539055826No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA801830.4910005080666993No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT790120.4838298909166039No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT697940.42738347854292325No Hit
TTGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT664300.40678402842087275No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT651130.398719380439083No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA633850.3881379744310856No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT615730.37704219451992166No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT579560.3548934991895243No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT569060.3484638253999426No Hit
GCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT529950.32451482140846233No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT516010.3159786640154366No Hit
TGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT467310.2861572246294717No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC431210.2640513937910049No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT413900.2534516172864658No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT399970.24492158339228734No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA379420.23233779326124873No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA378760.23193364233733216No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT372150.22788600959931926No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT366420.22437724475986176No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT362440.22194009221866792No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT352870.21607990382187772No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAAT343290.21021359192624026No Hit
GCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT333880.20445137951100556No Hit
GCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT330590.20243674839026993No Hit
CCCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT310580.19018362719698126No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA307060.18802815560275957No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT306280.18755052269267636No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT305010.18677283833907932No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT291960.17878167234345627No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT277880.1701597859665695No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT277700.17004956298731952No Hit
TTGCCAAGAATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT274680.16820026633545887No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT271700.1663754636789871No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT264550.16199716200322428No Hit
TCTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT263480.16134194762657167No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT262020.16044791679487744No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT256330.15696364595080883No Hit
GGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA256320.15695752245196162No Hit
AGGCCGAAGTGGAGAAGGGTTCCATGTGCTGTAGGCACCATCAAT238340.14594747152465876No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT236700.14494321771371454No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT236320.14471052475752016No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGCTGTAGGCACCATCAAT234200.14341234300190936No Hit
TGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGCTGTAGGCACCATC231170.14155692285120147No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT229050.1402587410955907No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCA227890.1395484152293131No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT224510.13747867261895247No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAAT224060.13720311517082756No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT222500.13624784935066114No Hit
TTGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT219060.1341413657472172No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT217470.1331677294305091No Hit
CAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT213710.1308652938639541No Hit
TCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT212500.1301243505034404No Hit
TCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT211970.1297998050645377No Hit
GCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGCTGTAGGCACCATCAA211500.12951200061871831No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAAT210970.1291874551798156No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT206760.12660946216513572No Hit
CCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT203780.12478465950866392No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT197640.1210248312164704No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA196660.12042472832944277No Hit
GACAGGTTAGTTTTACCCTACTGATGATGCTGTAGGCACCATCAAT190700.11677512301649921No Hit
CGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCA187020.114521675440722No Hit
TCAGGTTAGTTTTACCCTACTGATGATGCTGTAGGCACCATCAAT182820.11194980592488929No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAAT175320.10735718178947373No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT171540.10504249922522431No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT171300.10489553525289103No Hit
AGGTTAGTTTTACCCTACTGATGATGTGCTGTAGGCACCATCAAT169350.10370145297768298No Hit
ACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT168800.10336466054108583No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT166970.10224406025204444No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT166930.10221956625665554No Hit
GCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT164430.10068869154485037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)