FastQCFastQC Report
Wed 6 Sept 2023
SRR13762834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15578837
Total Bases734.4 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT7886695.062438229503268No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT5079183.260307556976172No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT4928123.1633426808432494No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA4221822.709971225708312No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT4128062.649787015551931No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC3044011.9539391804407478No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2942281.8886390556624992No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT2671061.7145439033735315No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT2002051.2851087664631191No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1623881.042362789982333No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1623761.0422857624096074No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1621391.040764467848274No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT1384480.8886927823944752No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1378590.88491201236652No Hit
CGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1374520.8822994938582386No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1352200.8679723653312503No Hit
TGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT1285100.8249011142487722No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1281200.8223977181351856No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1191110.7645692679113338No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1160080.7446512213973353No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT1110260.7126719407873643No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT1071770.6879653468355822No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT1047220.6722067892487739No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1045360.6710128618715249No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT1031500.6621161772217015No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT932860.5987995124411405No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT898930.5770199662529366No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT848700.5447775081028192No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT835630.536387921640107No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT830070.5328189774371476No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN783660.5030285636854663No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACC770290.49444640829093983No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT755410.4848949892729476No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA753980.4839770773646325No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT689520.4426004328821208No Hit
TTGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT629990.4043883378457583No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT624160.4006460816041659No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT597880.38377704317722816No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA596830.38310305191587796No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT545920.35042410418698133No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT540620.34702205305826106No Hit
GCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT499590.3206850421504506No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT493830.31698771865961495No Hit
TGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT441690.2835192383102795No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC406470.26091164571527387No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT394480.25321530740709336No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT382440.24548687427694377No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA362230.23251414723704983No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA360450.2313715715749513No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT357070.2292019616098429No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAAT350430.22493976925235173No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT349380.2242657779910015No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT348700.22382928841222233No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAAT330100.2118900146397321No Hit
GCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT316860.2033913057823251No Hit
GCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT316510.20316664202854168No Hit
CCCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT294210.18885235143034104No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT294160.18882025660837198No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA293740.1885506601038319No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT293070.1881205894894465No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG289910.18609219674100191No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT275670.1769515914442137No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT263380.16906268420421883No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT260870.16745152414137204No Hit
TTGCCAAGAATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT260100.16695726388304852No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT259610.16664273462775173No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT257750.1654488072505027No Hit
TCTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT254600.16342683346645195No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAAT254080.16309304731797375No Hit
GGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA244060.15666124499537418No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT243490.15629536402492689No Hit
AGGCCGAAGTGGAGAAGGGTTCCATGTGCTGTAGGCACCATCAAT231880.14884294636371123No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAAT227700.14615981924709784No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT227560.14606995374558449No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGCTGTAGGCACCATCAAT223300.1433354749138206No Hit
TGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGCTGTAGGCACCATC220970.1418398562100624No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT218370.1401709254676713No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT215720.13846989990331113No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAAT215670.1384378050813421No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCA214860.1379178689654433No Hit
TCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT211370.13567765039200294No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT209630.13456075058747966No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT209480.13446446612157248No Hit
TTGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT208230.13366209557234598No Hit
CAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAAT205880.1321536389398002No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAAT204220.1310880908504274No Hit
TCCCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAAT201860.1295732152534878No Hit
GCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGCTGTAGGCACCATCAA200800.12889280502774372No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT196670.1262417727330994No Hit
CCCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAAT193090.12394378348011471No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT187540.12038125824154909No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA186980.12002179623549564No Hit
GACAGGTTAGTTTTACCCTACTGATGATGCTGTAGGCACCATCAAT183900.11804475520220156No Hit
CGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCA178170.11436668860454731No Hit
TCAGGTTAGTTTTACCCTACTGATGATGCTGTAGGCACCATCAAT170850.10966800666827699No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAAT169920.10907104297965248No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAAT167930.10779366906528388No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT165940.1065162951509153No Hit
ACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT162390.10423756279111208No Hit
AGGTTAGTTTTACCCTACTGATGATGTGCTGTAGGCACCATCAAT160590.10308214920022592No Hit
GCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAAT159450.10235038725933136No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT159360.10229261657978705No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAAT158810.10193957353812738No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)