FastQCFastQC Report
Wed 6 Sept 2023
SRR13769802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13769802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84566620
Total Bases8.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA59357687.019043684139204No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT54940916.496760778661841No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA28195663.3341358564407564No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA20595932.4354680369157475No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA16863771.994140241149522No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA15585561.8429919511977657No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC14583901.7245456895403883No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT14569261.7228145100277155No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA13392301.583639029205613No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT12004171.4194927029128042No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG11854091.401745747908572No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG10779191.2746388586891613No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT10425171.2327760054735544No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA10141721.1992580524088583No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGAT9889251.1694034833129194No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA8698821.028635175439198No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA7421520.8775944929571502No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA7255910.8580111159698709No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATA6521660.771186077911119No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG6453230.7630942326889735No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA6437230.7612022332215713No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCTGTAGGCACCATCAATAGA5532620.6542321308336552No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATA5121490.6056160220190898No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATA4950970.5854520376952513No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG4729640.559279772562744No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT4308680.5095012665753935No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC3695810.4370294094762212No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA3358500.39714251320438254No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGAT3240120.3831440821449409No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAG3164100.37415471967544645No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3054750.3612240858154198No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG2783260.3291204023526067No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2597210.30711999604572116No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAG2566940.3035405695533297No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA2533640.29960284566179896No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAG2426940.28698557421356086No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT2235750.26437736307777226No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA2211870.2615535538726746No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA2182630.25809592484599714No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC2103990.24879674746371558No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT2094960.2477289502643005No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG2088510.24696623797900402No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA1967500.23265680950710815No Hit
CCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCGG1899730.2246430092629929No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT1833300.21678766397427257No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC1816220.21476795454282077No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATAGA1804330.21336196243860756No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1621350.19172458352952973No Hit
GGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT1561090.18459884053542638No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1549740.183256703413238No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA1528960.18079946910494943No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGA1395630.16503320104315392No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1347940.15939386013062837Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG1304330.1542369790822904No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1294720.15310059690218197No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA1294520.15307694690883944No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATCG1285850.1520517196974409No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG1274200.15067410758523872No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT1256600.14859290817109636No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGCTGTAGGCACCATCAATAGA1253920.1482759982603065No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATAG1230830.14554560652891177No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCTGTAGGCACCATCAATAGAT1188340.14052116544329193No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATC1166250.13790902367860983No Hit
GGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATC1154540.13652431656840489No Hit
TATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGA1116210.1319917953443096No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCTGTAGGCACCATCAATAGAT1086590.12848923133028137No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAGA1047850.12390822761983392No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAGAT1017730.12034653862244937No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATCGG1015730.1201100386890241No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGCTGTAGGCACCATCAAT1001990.11848528414639252No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGATC998130.11802883927488174No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA997430.1179460642981829No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA974060.11518256257610864No Hit
GGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGAT947310.11201937596654567No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT944800.11172256855009695No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA927450.10967093162763275No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA925640.10945689918788289No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT906130.10714984233731939No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA873100.10324404593680107No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT870940.10298862600870178No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATAG863320.10208756126235151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)