FastQCFastQC Report
Wed 6 Sept 2023
SRR13769803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13769803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71699287
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1083801715.115934137531939No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT998073413.920269527924315No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA23379433.2607618538800813No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA17954522.50414205653119No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT14742062.05609576005965No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATA11974061.670038922423315No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAA11112021.5498089960085657No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT9995541.3940919663538636No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA9888401.3791490004635611No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA9874681.377235452843485No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT7383461.0297815095427658No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATA5551610.77429082384041No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC4937880.6886930409782178No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATA4792180.6683720578699757No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA4725470.6590679207172591No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAG4669350.6512407856998634No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAG4459310.6219462126589906No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCACCATCAATA4435240.6185891360398047No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA4239250.5912541361812984No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG4132810.5764088002716122No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC4124390.5752344510762011No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA3994290.5570892218216897No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA3554820.4957957252768776No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA3267120.4556698032436501No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAGA3204530.44694028826255977No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGAT3153830.4398690882379346No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA2993990.4175759795212468No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2910540.4059370911177959No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2685620.37456718363182606No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAG2664240.37158528508100785No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA2622830.36580977437055967No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT2562460.3573898858994232No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA2468900.3443409416330737No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGA2122260.2959945752319685No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATAG1946130.27142947739494255No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCTGTAGGCACCATCAATAGAT1897620.2646637197382451No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT1775780.24767052425500408No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG1767780.24655475304796265No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1751310.2442576590754661No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCA1732130.24158259760658432No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATA1642480.22907898651767625No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATAGA1626450.22684325996156698No Hit
CGTACGCAGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAAT1539170.21467019609274496No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAAT1521070.2121457637368137No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1520320.2120411601861536No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA1411330.19684017220422287No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATCG1406360.19614699934184843No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1381580.19269089802803757No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGA1258920.17558333599607484No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1206650.16829316587206788Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAA1206310.1682457455957686No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATAG1189830.16594725690926326No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCTGTAGGCACCATCAATAGA1189320.1658761264948144No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1179240.16447025477394217No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATAG1156900.161354463678279No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAG1108950.1546668100060744No Hit
TATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGA1104580.15405731998422803No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAAT1103170.15386066530898695No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC1064330.1484435960988008No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1059610.14778529108664637No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC1055880.1472650627613633No Hit
GTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1016500.14177267899470186No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1012270.14118271496897872No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAATA996530.13898743511912467No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAGAT988580.13787863748212725No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT967150.13488976536126504No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGA966770.13483676622893057No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA963840.13442811502435162No Hit
ACCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA963280.13435001103985872No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATCA930600.12979208565909447No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT924910.12899849338808628No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATAG920100.1283276359498526No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA891030.12427320232626582No Hit
GCCGCCTGGATACCACAGCTAGGAATAATGGAATCTGTAGGCACCATCAA890520.12420207191181692No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATTCTGTAGGCACCATCAAT874140.1219175303653996No Hit
CCGCCTGGATACCACAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT835030.11646280387697579No Hit
ATATACGTACACCCTCTAACCTAGAGAAGGTTCTGTAGGCACCATCAATA825630.11515177270870211No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGTACCATCAA806240.11244742224563545No Hit
CTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG802160.1118783789300443No Hit
AACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCCTGTAGGCACCATCA801010.11171798681903211No Hit
ATATACGTACACCCTCTAACCTAGAGAAGGTTACTGTAGGCACCATCAAT736940.10278205416463905No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATAGA718750.10024506938262859No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)