FastQCFastQC Report
Wed 6 Sept 2023
SRR13769804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13769804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53907779
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA951991817.65963684016735No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT606048711.242323672804254No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC22066194.093322041703852No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA21115303.916930059389017No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA19850853.682372074724132No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA10120601.8773913872430172No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT9134371.6944437647857837No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA8952841.6607695894872612No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG7997201.483496472744685No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT7571731.4045709432770361No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA6935421.2865341753367356No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATA6850801.2708369973839955No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA6219161.1536665237126538No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT5679321.0535251322448287No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG5252250.9743027996015196No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA5049940.9367738930591075No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA4912690.9113137456469873No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG3781980.7015647964276176No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA3389720.6287997878747704No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATA3243870.6017443233934754No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATA2982950.5533431455226526No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2695600.5000391501938894No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAG2674460.49611763823547617No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGAT2485690.46110042856709044No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG2434200.45154893137036867No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA2224970.4127363510932253No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAG2012380.37330048414719513No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCTGTAGGCACCATCAATAGA1779950.3301842578229758No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA1757950.32610321415764504No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1749930.3246154882396472No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA1587940.29456602172387775No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1451570.2692691160583707No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT1440310.26718036370966053No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAG1294510.24013417432760492No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAA1148240.21300079901269905No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA1136240.21077477519524596No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA1102400.2044973880300281No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC1049220.19463239247901495No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA902110.16734319549688736No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG894340.16590184507508648No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA883860.16395778427451074No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA856930.15896221582417633No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATAGA851090.1578788842330158No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGTACCATCAA837160.1552948415849223No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA833850.1546808300152748No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA813600.1509244148233226No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT802090.14878928697841548No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA794650.14740915221159454No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAGAT771770.14316486679965057No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATTAA757730.1405604189332304No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGA714510.13254302315070335No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA703060.13041902542488348No Hit
GTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA701400.1301110921301358No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACTATCAA698000.1294803853818574No Hit
ACCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA687510.1275344695614338No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAG686840.12741018323162598No Hit
GCCGCCTGGATACCACAGCTAGGAATAATGGAATCTGTAGGCACCATCAA671670.12459611812239565No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT660320.12249067059505456No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA655510.12159840604822544No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT648930.12037780298832196No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA647940.12019415602338207No Hit
NCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA647730.12015520060657664No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAAT647490.12011068013022758No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC646200.11987138256985139No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA645860.11980831189502353No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG645630.11976564643852235No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA634390.11768060412950793No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA627600.11642104565279901Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGA607460.1126850356791735No Hit
TATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGA589680.10938681038964711No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT572190.10614238067570916No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATA571450.10600510920696622No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATAG568980.10554691930454044No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA563410.1045136732492726No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATTAAT558980.10369189945666282No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAGA542790.10068862232294898No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)