FastQCFastQC Report
Thu 7 Sept 2023
SRR13769806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13769806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88080182
Total Bases8.8 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT71028698.064094372557042No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA67677367.683608101536393No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC38003884.314691357018313No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA35315454.009466056734532No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA32886853.7337400142974273No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA22284042.5299720656798823No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT21255372.41318415986016No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA19802032.2481822301411687No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT13732021.5590362880948634No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA12685411.440211601742603No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGAT12259681.3918772329512217No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA12095021.3731829028237021No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA10868381.2339188854082976No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG10446381.18600799439765No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG9172531.0413840879665757No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT8551500.9708767404681339No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATA8423250.9563161438517465No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATA7459220.8468670057925176No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6594590.7487030396917209No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT6022680.6837724290805848No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATA5420960.6154574022110898No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG5282340.5997194692445118No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA4661350.5292166630627535No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA4402450.4998229908289699No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG4352390.49413953299960256No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC4038370.4584879263759923No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAG3743340.4249923098478612No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT3186860.361813512147375No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAG3082210.3499322923742369No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC2923260.3318862352032833No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2904480.3297540870203924No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAG2867700.3255783463299383No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2764120.3138186067780832No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA2494950.28325895148581776No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA2427780.2756329454450946No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATA2411710.273808471467509No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA2338840.26553532779939076No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA2264150.2570555542221745No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2222070.25227808907116017Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC2072550.2353026473083355No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA2014590.2287222794339821No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA1995980.22660943184699595No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT1986020.225478643992811No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1947730.22113146859755578No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1946590.22100204107207683No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA1858680.21102136233097246No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG1834480.20827386573747086No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGA1680260.19076481926433803No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG1664720.18900051773280852No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1646500.18693194798348622No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT1597700.18139154162964832No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA1554350.1764698896739337No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCTGTAGGCACCATCAATAGAT1527850.17346126737113238No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1523980.1730218949820063No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCTGTAGGCACCATCAATAGA1513830.1718695358735748No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAA1412830.16040271124780373No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAGA1400810.1590380455844199No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGAT1393750.1582365031897868No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG1381030.15679236448444214No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC1286980.1461145936324246No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATAG1215160.13796065952724756No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATC1199990.13623836517504018No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1197130.1359136610321718No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTCTGTAGGCACCATCAATAGA1193700.13552424312656394No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT1185250.1345648899771801No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA1185070.13454445405210447No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT1134070.1287542752806755No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC1113720.12644388041795826No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1086740.12338076231495525No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATAG1066700.12110556265653492No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT1033130.11729426262992962No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA1020060.1158103874036046No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1015220.11526088808490428No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAA1003700.11395298888006386No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA978190.11105676416517848No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG963650.1094059955507358No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATA961500.1091618997789991No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAAT954030.10831380888836038No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAGA946540.10746344733937993No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAG944180.10719550965505498No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT938590.10656086064853953No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA912570.10360673414593988No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT907520.10303339291465134No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)