FastQCFastQC Report
Thu 7 Sept 2023
SRR13769808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13769808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69594317
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT853432012.262955321481206No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA699571510.05213543513905No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC25627253.6823768239581978No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA24898463.5776570664527108No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA24518213.523018984438054No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA12224871.7565902687140387No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA11430961.6425134253418998No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT11154471.6027846066798814No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATA9508511.3662767895257883No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT8904751.2795225794083158No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA8164651.173177689207008No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA7068441.0156633910208501No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT6907600.9925523085455382No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGAT5202660.7475696614710653No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAG3997700.5744290873635559No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATA3852430.553555256530501No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3652480.5248244623192437No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATA3624720.5208356308748602No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCTGTAGGCACCATCAATAGA3339080.4797920496870456No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAG3315970.47647137624757496No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA3232580.46448907602613587No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA2906620.41765191833120513No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCTGTAGGCACCATCAATAGAT2775250.3987753770182126No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA2756630.3960998712006902No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2519080.3619663369927174No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG2488290.35754212517093886No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC2480840.35647163546414284No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG2281190.32778394822094453No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA2008450.28859396665966275No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1897700.27268031095125195No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1883780.27068014763331893No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC1861250.26744281433209555No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1804680.25931427705512217No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1783380.25625368232294027No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1760760.2530034169312992No Hit
AAAGGTTTGGTCCTGGCCTTATAATTAATTCTGTAGGCACCATCAATAGA1574670.22626416464436314No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1558270.22390765039047655No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA1544550.22193622505124952No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAG1544370.22191036087041419No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA1517000.21797756848450714No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG1457760.2094653791918096No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT1451740.20860036603276097No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC1421000.20418333870565897No Hit
AAAGGTTTGGTCCTGGCCTTATAATTAATCTGTAGGCACCATCAATAGAT1392480.20008530294219284No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA1369130.19673014392827506No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT1344080.19313071209535687No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA1343090.1929884591007625No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT1327870.19080149892124093No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT1309620.1881791583643245No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG1304790.18748513617857618No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT1285410.18470042604197123No Hit
TATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGA1239850.17815391449275952No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGA1216020.1747297843299475No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1149370.1651528529261951No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAAT1145210.1645551029691117No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1138850.16364123524626298No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1133750.16290841678926168No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1095630.1574309580479107No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1086130.15606590405937887No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATAGA1068420.15352115604496844No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAA1058530.15210006299796003No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA994500.14289959911525535No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG976320.14028731685088597No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGA918570.1319892254995476No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA915460.13154234993067035No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGA906730.13028793716015633No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA902650.1297016823945553No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATAG883290.1269198460558209No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA850360.12218813786188892Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT845120.12143520281979346No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGTACCATCAAT826240.11872233762995332No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCTGTAGGCACCATCAATAG810230.11642186243454333No Hit
ACGAGACTCTGGCATGCTAACTAGTTACCTGTAGGCACCATCAATAGATC807800.11607269599326624No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT806620.11590314191890123No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA793830.11406534818065676No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAGA780330.11212553461800623No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA775280.11139990065568142No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA754190.1083694808011407No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT736620.10584484937182442No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGTACCATCAA723940.10402286152186822No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATTAAT716330.10292938143210745No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCTGTAGGCACCATCAATAGAT711860.10228708760802983No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATA711590.10224829133677682No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)