FastQCFastQC Report
Wed 6 Sept 2023
SRR13769811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13769811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37167468
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA29756498.006057878357492No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT28888477.7725149316063185No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGAT9122262.45436681347247No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA8691642.338507428055094No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA8154072.193872878292382No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT6948501.8695112618379064No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA5243131.4106772083586647No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA4914711.3223149879351481No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA4264861.1474712240284972No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATA4234181.1392166934804384No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT3525760.9486145249388525No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAG3122970.8402428704586495No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCTGTAGGCACCATCAATAGAT3061130.823604664164909No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT2667940.7178159136371625No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA2576060.6930953703921935No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2570290.6915429374957691No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC2346810.6314150859025425No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA2289440.6159795442616646No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT2210750.5948078034263728No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATA2173850.5848797663591181No Hit
ACGAGACTCTGGCATGCTAACTAGTTACCTGTAGGCACCATCAATAGATC2115140.5690836943748765No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG2065270.5556660464468551No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAG1999000.5378359376000539No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGA1953090.5254837375524208No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATA1854110.49885292159261424No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1762670.47425076144546624No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA1696500.4564475578481698No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1526560.41072477683978903No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATCG1487840.4003070642315479No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGA1463830.39384711382545623No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1456940.3919933421345785No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAG1390010.373985658641046No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1321470.3555448006304869No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATAGA1274990.3430392406606767No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1226550.3300063378005733No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCTGTAGGCACCATCAATAGA1190780.32038233005272243No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGATC1181220.31781018820006784No Hit
ATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAGATC1177200.3167285971699767No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1166210.31377171024940415No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT1161750.312571736121492No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1122630.30204640251523185No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA1088120.2927614009111409No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1038520.27941639715678235No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAGA1018010.27389813048335715No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC1014960.27307752037346206No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATA1012400.2723887459861404No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA882510.23744151740441397No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA840770.22621126626112925No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT819380.2204562333920621No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA796700.21435412280438365No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAGAT789910.21252725636301079No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATCGG781130.2101649754564933No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATC769700.2070897054380998No Hit
TATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGA766930.20634443002681807No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAG762190.2050691212002927No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATAG760400.20458751723415758No Hit
ATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATAGATCG744740.20037415516171295No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGAT676430.18199517922501474No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGAT645720.17373257710210446No Hit
AAAGGTTTGGTCCTGGCCTTATAATTAATCTGTAGGCACCATCAATAGAT638270.1717281360140002No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGA628170.16901070581401995No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAGA624340.16798023475798782No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA618820.16649506498532535No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC611660.1645686491207849No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG608070.16360275066356417No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC602970.16223058293882167No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATAG567770.15275993511314787No Hit
AACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCCTGTAGGCACCATCA561170.15098418864583404No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA547240.14723628738982167No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG537800.1446964318365728No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGAT534800.14388927435143015No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATA531650.14304175899203034No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA527640.14196285848688966No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA514900.13853512969998388No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAAT512460.13787864161206784No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTCTGTAGGCACCATCAATAGA499600.13441862652575634No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG495180.13322941449764616No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAA492050.13238728018814733No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATAG483700.13014069185450028No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAGA474780.12774074359867613No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG473180.12731025960660006No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT459600.12365652672385431No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGAT451660.12152024991317677No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT450650.12124850689317873No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA450550.12122160164367399No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGATCG437940.11782884968112436No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA431480.11609077056311719No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAAT428460.11527823202807358No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGATCG391580.1053555760107199No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA380440.1023583312158902Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGGCGTGGGAAATGTGGCGTACGGAAGACCTGTAGGCACCATCAATAG378930.10195206194836838No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAG374700.10081396989431726No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG374460.10074939729550585No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA374240.10069020574659539No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA372500.10022205440521266No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)