FastQCFastQC Report
Wed 27 Sept 2023
SRR13777340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13777340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12740
Total Bases653 kbp
Sequences flagged as poor quality0
Sequence length34-92
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCA6435.047095761381476No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGG6324.960753532182103No Hit
AACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAA4433.4772370486656197No Hit
ACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAG3032.378335949764521No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGC2872.2527472527472527No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGC2562.009419152276295No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACC2501.9623233908948194No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGG2341.8367346938775513No Hit
TCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGG2321.8210361067503924No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCG2311.8131868131868134No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGC2261.7739403453689166No Hit
TAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGC1891.4835164835164834No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGC1601.2558869701726845No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCG1551.2166405023547882No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGG1531.2009419152276295No Hit
ACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGGTTTGCGG1481.1616954474097332No Hit
CAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATA1481.1616954474097332No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGC1441.130298273155416No Hit
CCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGGT1381.0832025117739403No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACAC1341.0518053375196232No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGT1140.8948194662480378No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACC1040.8163265306122449No Hit
AGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCAC1020.8006279434850864No Hit
TCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAT990.7770800627943485No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCG890.6985871271585558No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGT860.6750392464678179No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTG860.6750392464678179No Hit
CTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGG850.6671899529042387No Hit
TTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCAC840.6593406593406593No Hit
ATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGG820.6436420722135008No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGG800.6279434850863422No Hit
AATCGTTTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGT770.6043956043956045No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTAC770.6043956043956045No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTG670.5259026687598116No Hit
GCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGGTTT660.5180533751962323No Hit
TTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCA640.5023547880690737No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCG630.49450549450549447No Hit
ATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGGTTTGCGGTGTA590.46310832025117743No Hit
CGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGGTT560.43956043956043955No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCAC510.4003139717425432No Hit
TAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGC500.39246467817896385No Hit
CCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGGTTTGCGGT480.3767660910518053No Hit
TAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCG470.36891679748822603No Hit
CCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGGTTTGCGGTG450.35321821036106754No Hit
TATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAG450.35321821036106754No Hit
ATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTA440.3453689167974882No Hit
TAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACC440.3453689167974882No Hit
TAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGC430.33751962323390894No Hit
AACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAT430.33751962323390894No Hit
TAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGG430.33751962323390894No Hit
AACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGGTTTGCG420.32967032967032966No Hit
TTTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGC420.32967032967032966No Hit
AGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGC420.32967032967032966No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACAC410.3218210361067504No Hit
AGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGG360.282574568288854No Hit
ACAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGC360.282574568288854No Hit
AGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGC350.27472527472527475No Hit
ACAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACC340.2668759811616954No Hit
ACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAT330.25902668759811615No Hit
TTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACA310.24332810047095763No Hit
ATGCACAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGC310.24332810047095763No Hit
ATCGTTTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTG290.22762951334379905No Hit
TTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGC270.2119309262166405No Hit
TTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGT270.2119309262166405No Hit
TAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTG260.20408163265306123No Hit
TAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGT260.20408163265306123No Hit
ATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGGT260.20408163265306123No Hit
TATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGG250.19623233908948193No Hit
TTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTAC240.18838304552590265No Hit
ACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAT240.18838304552590265No Hit
AAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCG240.18838304552590265No Hit
AAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGC240.18838304552590265No Hit
TTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCG230.1805337519623234No Hit
TGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTT230.1805337519623234No Hit
TTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGG220.1726844583987441No Hit
AGCTGATGCACAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGC220.1726844583987441No Hit
AGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCG220.1726844583987441No Hit
TTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGC220.1726844583987441No Hit
AGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGC210.16483516483516483No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGTGGCACAGG200.15698587127158556No Hit
TAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTAC200.15698587127158556No Hit
AGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACC200.15698587127158556No Hit
TGCACAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGC190.14913657770800628No Hit
TCGTTTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGC190.14913657770800628No Hit
ATGCACAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTA180.141287284144427No Hit
ATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCAC180.141287284144427No Hit
TATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGC180.141287284144427No Hit
TCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAT170.1334379905808477No Hit
TAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACAC170.1334379905808477No Hit
GCACAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGC170.1334379905808477No Hit
AGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACAC170.1334379905808477No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGTGGCACAGGCA170.1334379905808477No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGACACAGGCA170.1334379905808477No Hit
TCTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAG170.1334379905808477No Hit
TTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGC170.1334379905808477No Hit
TTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGC170.1334379905808477No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGACA170.1334379905808477No Hit
AGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGT160.12558869701726844No Hit
CATGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGGTTTGCGGTGT160.12558869701726844No Hit
TAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCG160.12558869701726844No Hit
TCAGCTGATGCACAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGCAGCC160.12558869701726844No Hit
TGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACA160.12558869701726844No Hit
TTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGT150.11773940345368916No Hit
TGATGCACAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGC150.11773940345368916No Hit
TGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTT150.11773940345368916No Hit
TGCTTCAGTCAGCTGATGCACAATCGTTTTTATAAGGGTTTGCGGTGTAA150.11773940345368916No Hit
TTTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGC150.11773940345368916No Hit
ATGCTTCAGTCAGCTGATGCACAATCGTTTTTAT150.11773940345368916No Hit
AGCTGATGCACAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCG140.10989010989010989No Hit
TTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTG140.10989010989010989No Hit
AGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATC140.10989010989010989No Hit
TTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCG130.10204081632653061No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGTGGC130.10204081632653061No Hit
TAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAAT130.10204081632653061No Hit
TATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCG130.10204081632653061No Hit
CCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAT130.10204081632653061No Hit
TGCACAATCGTTTTTATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTAC130.10204081632653061No Hit
TATAAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGC130.10204081632653061No Hit
AAGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCA130.10204081632653061No Hit
ATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGAT130.10204081632653061No Hit
TAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTATA130.10204081632653061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)