FastQCFastQC Report
Wed 6 Sept 2023
SRR13780863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13780863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22805409
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCGACGGATCGCTGGAATTCTCGGGTGCCAAG333510314.62417534366518No Hit
CTGATGATTCATGATAACTCGACGGATCGCATGGAATTCTCGGGTGCCAA6651622.916685247784857No Hit
ACCATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCCA3803951.668003410945184No Hit
CCATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCCAA3448101.5119658673957568No Hit
AGCGGAGGAAAAGAAACTAACAAGGATTCCCTTGGAATTCTCGGGTGCCA2949391.2932852903449354No Hit
AGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAACT2567271.1257285497488776RNA PCR Primer, Index 1 (100% over 25bp)
CATGCGGCGGGCAGCTGTGCTGGAGTGGAATTCTCGGGTGCCAAGGAACT2292141.0050861179468433RNA PCR Primer, Index 1 (100% over 25bp)
TCTGATGATTCATGATAACTCGACGGATCGCATGGAATTCTCGGGTGCCA2251800.98739733192244No Hit
ACTTTAAATAGGTAGGACGTGTCGGCTTGGAATTCTCGGGTGCCAAGGAA1999510.8767700680132507RNA PCR Primer, Index 1 (100% over 23bp)
CCATCGAGTCTTTGAACGCAAGTTGCGCCCTGGAATTCTCGGGTGCCAAG1909610.8373495954402748No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAG1746380.7657744704337467No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAAC1739560.7627839518247623RNA PCR Primer, Index 1 (100% over 24bp)
ACCATCGAGTCTTTGAACGCAAGTTGCGCCCTGGAATTCTCGGGTGCCAA1516260.6648685844660799No Hit
TCTGATGATTCATGATAACTCGACGGATCGCTGGAATTCTCGGGTGCCAA1375770.6032647781059309No Hit
AACCATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCC1278890.5607836281296249No Hit
AACCATCGAGTCTTTGAACGCAAGTTGCGCCCTGGAATTCTCGGGTGCCA1253260.5495450662603771No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGTGGAATTCTCGGGTGCCAAGGA1220010.5349651918104165RNA PCR Primer, Index 1 (100% over 22bp)
GATGTCGGCTCTTCCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1078690.4729974367045993RNA PCR Primer, Index 1 (100% over 31bp)
GCGGATTACGGTGGCGGCCTAAGCCCGGGCTTGGAATTCTCGGGTGCCAA1048110.4595883371352823No Hit
TGGTCGATGGAACAATGTAGGCAAGGGATGGAATTCTCGGGTGCCAAGGA1038870.4555366667618195RNA PCR Primer, Index 1 (100% over 22bp)
ACCTCCCGGGAAGTCCTCGTGTTGCATCCCTTGGAATTCTCGGGTGCCAA941360.41277926653277736No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTATGGAATTCTCGGGTGCCAAGGA811830.3559813375853071RNA PCR Primer, Index 1 (100% over 22bp)
ACCAAATCGAGGCAAACTCTGAATACTAGATTGGAATTCTCGGGTGCCAA743740.3261243856665758No Hit
GCTTTTTGCATGCGGCGGGCAGCTGTGCTGTGGAATTCTCGGGTGCCAAG718610.3151050700296583No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATACTGATCTCGTATGC680180.2982538046127566RNA PCR Primer, Index 27 (97% over 37bp)
GGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAA669500.29357070509018274RNA PCR Primer, Index 1 (100% over 23bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGA649570.28483155026949963RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAA626360.2746541401647302RNA PCR Primer, Index 1 (100% over 23bp)
GATGTCGGCTCTTCCTATCATTGTGAATGGAATTCTCGGGTGCCAAGGAA618800.27133913713189706RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGG598890.26260875216050716Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAA569320.24964252998049716No Hit
GATGTCGGCTCTTCCTATCATTGTGATGGAATTCTCGGGTGCCAAGGAAC568550.2493048907827086RNA PCR Primer, Index 1 (100% over 24bp)
ACTTTAAATAGGTAGGACGTGTCGGCTGTGGAATTCTCGGGTGCCAAGGA515240.2259288574916591RNA PCR Primer, Index 1 (100% over 22bp)
TAGGACTCCGCTGGCACCTTATGAGAAATCTGGAATTCTCGGGTGCCAAG512510.22473177306313605No Hit
CGGCGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAGG491780.21564182427072456Illumina Small RNA Adapter 2 (100% over 21bp)
CATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCCAAG490590.2151200182377786No Hit
ATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCCAAGG485470.212874936818717Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGATCCTTCGATGTCGGCTCTTCCTATTGGAATTCTCGGGTGCCAAGG481120.21096749459744396Illumina Small RNA Adapter 2 (100% over 21bp)
GAGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGGAA468280.20533725135120356RNA PCR Primer, Index 1 (100% over 23bp)
GAACCATCGAGTCTTTGAACGCAAGTTGCGCCCTGGAATTCTCGGGTGCC461400.20232042319433957No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGG455560.19975962720072243Illumina Small RNA Adapter 2 (100% over 21bp)
AGACGTCGCTGCCGGGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAACT439090.19253765630776454RNA PCR Primer, Index 1 (100% over 25bp)
GCGGATTACGGTGGCGGCCTAAGCCCGGGCTGGAATTCTCGGGTGCCAAG432550.18966991558888507No Hit
TTTAAATAGGTAGGACGTGTCGGCTGCTTTGGAATTCTCGGGTGCCAAGG423730.18580241205057976Illumina Small RNA Adapter 2 (100% over 21bp)
ACTTTAAATAGGTAGGACGTGTCGGCTGCTTGGAATTCTCGGGTGCCAAG421920.18500874068954432No Hit
AACTTTAAATAGGTAGGACGTGTCGGCTTGGAATTCTCGGGTGCCAAGGA409770.17968105724391964RNA PCR Primer, Index 1 (100% over 22bp)
TTGATCCTTCGATGTCGGCTCTTCCTATGGAATTCTCGGGTGCCAAGGAA406410.17820772256266046RNA PCR Primer, Index 1 (100% over 23bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCATGGAATTCTCGGGTGCCAA400350.17555045822681803No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCTGGAATTCTCGGGTGCCAAG394490.17298089238390768No Hit
CTGATGATTCATGATAACTCGACGGATCACTGGAATTCTCGGGTGCCAAG388750.1704639456367566No Hit
AGACGTCGCTGCCGTGATCGTGGTCTGTGGAATTCTCGGGTGCCAAGGAA382820.16786368532132004RNA PCR Primer, Index 1 (100% over 23bp)
TTTTGATCCTTCGATGTCGGCTCTTCCTATGGAATTCTCGGGTGCCAAGG357490.1567566711914704Illumina Small RNA Adapter 2 (100% over 21bp)
TGATGATTCATGATAACTCGACGGATCGCTGGAATTCTCGGGTGCCAAGG346520.15194640885414507Illumina Small RNA Adapter 2 (100% over 21bp)
AGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGAACTC345510.15150353146483803RNA PCR Primer, Index 1 (100% over 26bp)
GAGACGTCGCTGCCGGGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAAC338270.14832884602069624RNA PCR Primer, Index 1 (100% over 24bp)
TCTGGTGTCCTAGGCGTAGAGGAACAACACCATGGAATTCTCGGGTGCCA334850.14682920179155742No Hit
CGAGGCAAACTCTGAATACTAGATATGACCTTGGAATTCTCGGGTGCCAA330100.14474636258442022No Hit
TTTGAAATTTCATCGTGATGGGGATAGATCATGGAATTCTCGGGTGCCAA326740.14327302790316104No Hit
TTCTGGTGTCCTAGGCGTAGAGGAACAACACCTGGAATTCTCGGGTGCCA326110.14299677765042496No Hit
CGGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGGA324600.14233465402878764RNA PCR Primer, Index 1 (100% over 22bp)
GATGTCGGCTCTTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA318540.13967738969294521RNA PCR Primer, Index 1 (100% over 34bp)
CGGATAACCGTAGTAATTCTAGAGCTAATACGTGGAATTCTCGGGTGCCA316580.13881794446221069No Hit
TTAGTTGGTGGAGCGATTTGTCTGGTTTGGAATTCTCGGGTGCCAAGGAA314870.13806812234764132RNA PCR Primer, Index 1 (100% over 23bp)
AGCGCGCGACCTATACCCGGCCGTCGGGGCTGGAATTCTCGGGTGCCAAG304930.13370950724891625No Hit
TGTGGAGACGTCGCTGCCGGGATCGTGGTCTGGAATTCTCGGGTGCCAAG289790.1270707313339568No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTATCTGGAATTCTCGGGTGCCAAG286890.12579910318644144No Hit
CGGCGTTCGAATTGTAGTCTGGAGAAGCGTTGGAATTCTCGGGTGCCAAG282190.12373818860253723No Hit
TTTAAATAGGTAGGACGTGTCGGCTGCTTGGAATTCTCGGGTGCCAAGGA281810.12357156146596625RNA PCR Primer, Index 1 (100% over 22bp)
ACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGGAATTCTCGGGTGCCAA281170.12329092628858357No Hit
AGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGGAAC279840.12270773131058514RNA PCR Primer, Index 1 (100% over 24bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGGAATTCTCGGGTGCCAAGGA276430.12121247200609295RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGCGCTGTCTACGAGTCGGGTTGTTTTGGAATTCTCGGGTGCCAAGG273990.1201425503923214Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATTGGAATTCTCGGGTGCCA273600.11997153833110381No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTTGGAATTCTCGGGTGCCAAGGAA271350.11898493028561777RNA PCR Primer, Index 1 (100% over 23bp)
CCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAACTC270720.11870868003288167RNA PCR Primer, Index 1 (100% over 26bp)
TTGATCCTTCGATGTCGGCTCTTCCTATCTGGAATTCTCGGGTGCCAAGG266470.1168450870580747Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGG265820.11656006695604539Illumina Small RNA Adapter 2 (100% over 21bp)
CTGATGATTCATGAGAACTCGACGGATCGCTGGAATTCTCGGGTGCCAAG258410.11331083779291132No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATTGGAATTCTCGGGTGCCAAGGA258200.11321875437533262RNA PCR Primer, Index 1 (100% over 22bp)
ATGATTAACAGGGACAGTCGGGGGCTGGAATTCTCGGGTGCCAAGGAACT252270.11061849405989606RNA PCR Primer, Index 1 (100% over 25bp)
TACCAAATCGAGGCAAACTCTGAATACTAGATTGGAATTCTCGGGTGCCA251050.11008353325301029No Hit
CGCGGATTACGGTGGCGGCCTAAGCCCGGGCTTGGAATTCTCGGGTGCCA237520.10415073020615417No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTGGAATTCTCGGGTGCCAAGGA237330.10406741663786867RNA PCR Primer, Index 1 (100% over 22bp)
TCGGCGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAG234280.10273001462065424No Hit
ATGTCGGCTCTTCCTATCATTGTGAATGGAATTCTCGGGTGCCAAGGAAC232820.10208981562224997RNA PCR Primer, Index 1 (100% over 24bp)
AACGATGCCGACCAGGGATCAGCGGATGTGGAATTCTCGGGTGCCAAGGA232470.1019363432596188RNA PCR Primer, Index 1 (100% over 22bp)
TCTGATGATTCATGATAACTCGACGGATCGCATTGGAATTCTCGGGTGCC231970.10171709702728857No Hit
CTGGTCGATGGAACAATGTAGGCAAGGGATGGAATTCTCGGGTGCCAAGG230630.10112951712464353Illumina Small RNA Adapter 2 (100% over 21bp)
CATCGAGTCTTTGAACGCAAGTTGCGCCCTGGAATTCTCGGGTGCCAAGG230460.10105497340565127Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)