FastQCFastQC Report
Wed 6 Sept 2023
SRR13780864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13780864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34987489
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCGACGGATCGCTGGAATTCTCGGGTGCCAAG19697825.629961041216762No Hit
AGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAACT7948582.2718349407698275RNA PCR Primer, Index 1 (100% over 25bp)
ACTTTAAATAGGTAGGACGTGTCGGCTTGGAATTCTCGGGTGCCAAGGAA5357231.5311844756850084RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAG5029541.4375252822516071No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAAC4820171.3776838915190512RNA PCR Primer, Index 1 (100% over 24bp)
CCATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCCAA4436221.2679446644484833No Hit
AGCGGAGGAAAAGAAACTAACAAGGATTCCCTTGGAATTCTCGGGTGCCA3865801.1049092434155534No Hit
ACCATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCCA3712301.061036417903554No Hit
CATGCGGCGGGCAGCTGTGCTGGAGTGGAATTCTCGGGTGCCAAGGAACT2630440.7518230302266047RNA PCR Primer, Index 1 (100% over 25bp)
GGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAA2435650.6961488433765567RNA PCR Primer, Index 1 (100% over 23bp)
CGGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGGA2201830.629319240371894RNA PCR Primer, Index 1 (100% over 22bp)
CTGATGATTCATGATAACTCGACGGATCGCATGGAATTCTCGGGTGCCAA2104740.6015693209649884No Hit
CGGCGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAGG2031580.580658989274709Illumina Small RNA Adapter 2 (100% over 21bp)
CCATCGAGTCTTTGAACGCAAGTTGCGCCCTGGAATTCTCGGGTGCCAAG1857600.530932642808405No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGA1684990.48159786488250134RNA PCR Primer, Index 1 (100% over 22bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGG1677160.47935992205671Illumina Small RNA Adapter 2 (100% over 21bp)
ACCAAATCGAGGCAAACTCTGAATACTAGATTGGAATTCTCGGGTGCCAA1659710.47437242495453164No Hit
CGGATAACCGTAGTAATTCTAGAGCTAATACGTGGAATTCTCGGGTGCCA1613640.46120486097187485No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGG1448090.41388794720307026Illumina Small RNA Adapter 2 (100% over 21bp)
ACCATCGAGTCTTTGAACGCAAGTTGCGCCCTGGAATTCTCGGGTGCCAA1424780.4072255656872089No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGTGGAATTCTCGGGTGCCAAGGA1284310.36707692855580465RNA PCR Primer, Index 1 (100% over 22bp)
GCGGATTACGGTGGCGGCCTAAGCCCGGGCTTGGAATTCTCGGGTGCCAA1221820.3492162584174017No Hit
ACTTTAAATAGGTAGGACGTGTCGGCTGTGGAATTCTCGGGTGCCAAGGA1175540.3359886729796471RNA PCR Primer, Index 1 (100% over 22bp)
TCGGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGG1072510.3065410038428308Illumina Small RNA Adapter 2 (100% over 21bp)
AACCATCGAGTCTTTGAACGCAAGTTGCGCCCTGGAATTCTCGGGTGCCA1037470.29652599533507534No Hit
GCGGATTACGGTGGCGGCCTAAGCCCGGGCTGGAATTCTCGGGTGCCAAG1003160.28671963283789814No Hit
GACATTGTCAGGTGGGGAGTTTGGCTGGGTGGAATTCTCGGGTGCCAAGG948700.27115406881585585Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCTGGAATTCTCGGGTGCCAAG929100.2655520663400566No Hit
CGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAA915420.26164209726511095RNA PCR Primer, Index 1 (100% over 23bp)
AGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGAACTC882870.25233877172494434RNA PCR Primer, Index 1 (100% over 26bp)
CCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAACTC861260.24616227817892278RNA PCR Primer, Index 1 (100% over 26bp)
GCCCATTAAAGCGGTACGTGAGCTGGTGGAATTCTCGGGTGCCAAGGAAC846870.24204937942245583RNA PCR Primer, Index 1 (100% over 24bp)
GAGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGGAA829250.23701329352329342RNA PCR Primer, Index 1 (100% over 23bp)
GATGTCGGCTCTTCCTATCATTGTGATGGAATTCTCGGGTGCCAAGGAAC800690.2288503756299859RNA PCR Primer, Index 1 (100% over 24bp)
TGGTCGATGGAACAATGTAGGCAAGGGATGGAATTCTCGGGTGCCAAGGA794110.22696970337025327RNA PCR Primer, Index 1 (100% over 22bp)
CGGCGTTCGAATTGTAGTCTGGAGAAGCGTTGGAATTCTCGGGTGCCAAG782490.22364851618817227No Hit
GATGTCGGCTCTTCCTATCATTGTGAATGGAATTCTCGGGTGCCAAGGAA757250.2164345089183165RNA PCR Primer, Index 1 (100% over 23bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCATGGAATTCTCGGGTGCCAA745430.2130561584456661No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAGG740240.21157277105539068Illumina Small RNA Adapter 2 (100% over 21bp)
CATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCCAAG728560.20823443488613888No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAA727890.20804293786273143No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATGGAATTCTCGGGTGCCA727480.20792575311706424No Hit
GCTTTTTGCATGCGGCGGGCAGCTGTGCTGTGGAATTCTCGGGTGCCAAG701770.20057741211437036No Hit
AACCATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCC695090.19866815820935307No Hit
GACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAACTC684530.1956499364672898RNA PCR Primer, Index 1 (100% over 26bp)
AGACGTCGCTGCCGTGATCGTGGTCTGTGGAATTCTCGGGTGCCAAGGAA676450.1933405395282868RNA PCR Primer, Index 1 (100% over 23bp)
AACTTTAAATAGGTAGGACGTGTCGGCTTGGAATTCTCGGGTGCCAAGGA664060.1897992736775137RNA PCR Primer, Index 1 (100% over 22bp)
GGCGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAGGA661610.1890990233680388RNA PCR Primer, Index 1 (100% over 22bp)
TTAGTTGGTGGAGCGATTTGTCTGGTTTGGAATTCTCGGGTGCCAAGGAA655740.18742128078982748RNA PCR Primer, Index 1 (100% over 23bp)
TCGGCGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAG655460.18734125218303035No Hit
CGAGGCGCTGTCTACGAGTCGGGTTGTTTTGGAATTCTCGGGTGCCAAGG636630.18195932837592318Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTGGAATTCTCGGGTGCCAAGGA621230.17755775500208087RNA PCR Primer, Index 1 (100% over 22bp)
CGCCCATTAAAGCGGTACGTGAGCTGGTGGAATTCTCGGGTGCCAAGGAA593880.16974067501671813RNA PCR Primer, Index 1 (100% over 23bp)
AGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGGAAC592770.16942341875405806RNA PCR Primer, Index 1 (100% over 24bp)
TCTGATGATTCATGATAACTCGACGGATCGCTGGAATTCTCGGGTGCCAA590370.1687374592672255No Hit
CCCCTGTTTTTGGTCCCAAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC564110.16123191921546587RNA PCR Primer, Index 1 (100% over 27bp)
CGAGGCGCTGTCTACGAGTCGGGTTGTTTGGAATTCTCGGGTGCCAAGGA548390.15673888457671253RNA PCR Primer, Index 1 (100% over 22bp)
CTGATGATTCATGAGAACTCGACGGATCGCTGGAATTCTCGGGTGCCAAG533560.152500226580993No Hit
CCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAACT530840.1517228058292494RNA PCR Primer, Index 1 (100% over 25bp)
TGGTCGATGGAACAATGTAGGCAAGGGTGGAATTCTCGGGTGCCAAGGAA513920.14688679144707983RNA PCR Primer, Index 1 (100% over 23bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGT499280.1427024385774012No Hit
TTCGGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAG492470.1407560285335138No Hit
GTGAACCATCGAGTCTTTGAACGCAAGTTGGAATTCTCGGGTGCCAAGGA491260.14041019062556903RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGCAAACTCTGAATACTAGATATGACCTTGGAATTCTCGGGTGCCAA485700.13882105114774027No Hit
AATCGTTCGTCTGACTTGGGTATAGGGTGGAATTCTCGGGTGCCAAGGAA479090.13693180439442224RNA PCR Primer, Index 1 (100% over 23bp)
ATGATTAACAGGGACAGTCGGGGGCTGGAATTCTCGGGTGCCAAGGAACT477230.136400185792127RNA PCR Primer, Index 1 (100% over 25bp)
TTCGGGAAAAGGATTGGCTCTGAGGGCTGTGGAATTCTCGGGTGCCAAGG464090.13264455760171873Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGAAGTGTTCGGATCGCGGCGACGTGGGTGGAATTCTCGGGTGCCAAG456090.13035802597894353No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGA438230.12525334413109784RNA PCR Primer, Index 1 (100% over 22bp)
ATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCCAAGG435180.12438160394991479Illumina Small RNA Adapter 2 (100% over 21bp)
GATGTCGGCTCTTCCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC432260.12354701990760182RNA PCR Primer, Index 1 (100% over 31bp)
GGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGGAA431220.12324977079664105RNA PCR Primer, Index 1 (100% over 23bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCCATGGAATTCTCGGG428740.12254094599358073No Hit
AGCGCGCGACCTATACCCGGCCGTCGGGGCTGGAATTCTCGGGTGCCAAG427280.12212365397242426No Hit
TAGGACTCCGCTGGCACCTTATGAGAAATCTGGAATTCTCGGGTGCCAAG412280.11783640717972074No Hit
ACCCCTGTTTTTGGTCCCAAGGCTTGGAATTCTCGGGTGCCAAGGAACTC385800.11026798750833476RNA PCR Primer, Index 1 (100% over 26bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAAC380350.10871028784031915RNA PCR Primer, Index 1 (100% over 24bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAG369140.10550628540390537No Hit
TTCGGCGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAA362800.10369420909285601No Hit
CGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAGGAAC360950.10316544865508925RNA PCR Primer, Index 1 (100% over 24bp)
TTGTGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAA358680.10251664530712679No Hit
TTTGAAATTTCATCGTGATGGGGATAGATCTGGAATTCTCGGGTGCCAAG358200.10237945340976026No Hit
ACTTTAAATAGGTAGGACGTGTCGGCTGCTTGGAATTCTCGGGTGCCAAG357470.10217080739918204No Hit
TCGGGAAAAGGATTGGCTCTGAGGGCTGTGGAATTCTCGGGTGCCAAGGA356580.1019164307561483RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)