FastQCFastQC Report
Thu 14 Sept 2023
SRR13780865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13780865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71754751
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAAC45434546.331920795042548RNA PCR Primer, Index 1 (100% over 24bp)
GGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAA30244394.214966894665971RNA PCR Primer, Index 1 (100% over 23bp)
AGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAACT20699992.884825006221539RNA PCR Primer, Index 1 (100% over 25bp)
ACTTTAAATAGGTAGGACGTGTCGGCTTGGAATTCTCGGGTGCCAAGGAA19036192.652951858198212RNA PCR Primer, Index 1 (100% over 23bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGA18584582.59001386542335RNA PCR Primer, Index 1 (100% over 22bp)
GAGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGGAA10998871.532842055294708RNA PCR Primer, Index 1 (100% over 23bp)
CTGATGATTCATGATAACTCGACGGATCGCTGGAATTCTCGGGTGCCAAG10439321.4548611561623286No Hit
CGGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGGA8577731.1954232828429716RNA PCR Primer, Index 1 (100% over 22bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGG7593531.058261633435255Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGG6131300.8544800050940181Illumina Small RNA Adapter 2 (100% over 21bp)
GGAGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGGA5535430.7714374202204395RNA PCR Primer, Index 1 (100% over 22bp)
TGGAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGAA4286850.5974308237791808RNA PCR Primer, Index 1 (100% over 23bp)
AGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGGAAC4163000.5801706426380046RNA PCR Primer, Index 1 (100% over 24bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTGGAATTCTCGGGTGCCAAGGA3895750.5429257220891199RNA PCR Primer, Index 1 (100% over 22bp)
GGAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGAAC3833840.5342977219724447RNA PCR Primer, Index 1 (100% over 24bp)
GAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGAACT3781840.527050815074252RNA PCR Primer, Index 1 (100% over 25bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGA3752040.5228977799672108RNA PCR Primer, Index 1 (100% over 22bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAAC3704280.5162417747084093RNA PCR Primer, Index 1 (100% over 24bp)
AGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGAACTC3547090.49433521133673786RNA PCR Primer, Index 1 (100% over 26bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGG3376410.47054863307936223Illumina Small RNA Adapter 2 (100% over 21bp)
AACTTTAAATAGGTAGGACGTGTCGGCTTGGAATTCTCGGGTGCCAAGGA3180780.44328493314679607RNA PCR Primer, Index 1 (100% over 22bp)
TCGGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGG3169780.44175193361063997Illumina Small RNA Adapter 2 (100% over 21bp)
AACGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3169460.44170733726049727RNA PCR Primer, Index 1 (100% over 31bp)
CGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAGGAAC2804260.3908117526601131RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGATTACGGTGGCGGCCTAAGCCCGTGGAATTCTCGGGTGCCAAGGA2762350.3849710244273581RNA PCR Primer, Index 1 (100% over 22bp)
CGTGAACCATCGAGTCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACT2420670.3373532715624642RNA PCR Primer, Index 1 (100% over 25bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAA2381850.33194317683577496RNA PCR Primer, Index 1 (100% over 23bp)
GCGGATTACGGTGGCGGCCTAAGCCCGGGCTGGAATTCTCGGGTGCCAAG2353630.3280103362075635No Hit
ACTTTAAATAGGTAGGACGTGTCGGCTGTGGAATTCTCGGGTGCCAAGGA2290780.319251334312344RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGCAAACTCTGAATACTAGATATGACCTTGGAATTCTCGGGTGCCAA2186240.30468226417509275No Hit
AGCCCAGCTTGAAAATCGGACGTGGAATTCTCGGGTGCCAAGGAACTCCA2097620.292331862457442RNA PCR Primer, Index 1 (100% over 28bp)
GCGGATTACGGTGGCGGCCTAAGCCCGTGGAATTCTCGGGTGCCAAGGAA2053960.2862472479348441RNA PCR Primer, Index 1 (100% over 23bp)
GTGAACCATCGAGTCTTTGAACGCAAGTGGAATTCTCGGGTGCCAAGGAA1915440.26694260286681226RNA PCR Primer, Index 1 (100% over 23bp)
GGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGGAA1897490.2644410263509938RNA PCR Primer, Index 1 (100% over 23bp)
CCCGCGACGTCGCGAGAAGTCCACTAAACCTTGGAATTCTCGGGTGCCAA1854390.2584344554411456No Hit
AAACGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1837450.2560736361554652RNA PCR Primer, Index 1 (100% over 30bp)
CGGAGGTGTGCGAAGGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1654880.230630024763099RNA PCR Primer, Index 1 (100% over 28bp)
CCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAACT1647880.2296544796037269RNA PCR Primer, Index 1 (100% over 25bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGGAATTCTCGGGTGCCAAGGA1625040.2264714151122899RNA PCR Primer, Index 1 (100% over 22bp)
GACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAACTC1601350.22316989156578637RNA PCR Primer, Index 1 (100% over 26bp)
CGGCGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAGG1573010.21922032730627133Illumina Small RNA Adapter 2 (100% over 21bp)
TACCGTGCGCTGGATTATGACTGTGGAATTCTCGGGTGCCAAGGAACTCC1565820.218218303064002RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGAAAAATAGCTCGACGCCAGGATGGAATTCTCGGGTGCCAAGGAAC1526980.21280542106542882RNA PCR Primer, Index 1 (100% over 24bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCTGGAATTCTCGGGTGCCAAG1488860.2074928808546768No Hit
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGAATTCTCGGGTGCCAAGG1485130.20697305464832566Illumina Small RNA Adapter 2 (100% over 21bp)
AGGCCTACGGGTCCTGAACTTCTTTTCCCAGATGGAATTCTCGGGTGCCA1439620.2006306174764651No Hit
GCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGGAAC1426850.198850944378582RNA PCR Primer, Index 1 (100% over 24bp)
GATGTCGGCTCTTCCTATCATTGTGAAGTGGAATTCTCGGGTGCCAAGGA1381770.1925684335522257RNA PCR Primer, Index 1 (100% over 22bp)
CGTGAACCATCGAGTCTTTGAACGCAATGGAATTCTCGGGTGCCAAGGAA1376710.19186325376559385RNA PCR Primer, Index 1 (100% over 23bp)
CGTGAACCATCGAGTCTTTGAACGCAAGTGGAATTCTCGGGTGCCAAGGA1342380.18707890157684473RNA PCR Primer, Index 1 (100% over 22bp)
AACGGAGGTGTGCGAAGGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTC1341930.18701618795945651RNA PCR Primer, Index 1 (100% over 26bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAGG1336860.18630961453688272Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCATTAAAGCGGTACGTGAGCTGGTGGAATTCTCGGGTGCCAAGGAAC1316490.1834707781231099RNA PCR Primer, Index 1 (100% over 24bp)
CGCTTGTGGAGACGTCGCTGCCGTGATCTGGAATTCTCGGGTGCCAAGGA1314610.18320877456602142RNA PCR Primer, Index 1 (100% over 22bp)
GCGGCGGGTAGAATCCTTTGCAGACGTGGAATTCTCGGGTGCCAAGGAAC1300390.18122702425655413RNA PCR Primer, Index 1 (100% over 24bp)
GTGAACCATCGAGTCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACTC1253410.17467972260122538RNA PCR Primer, Index 1 (100% over 26bp)
GAAGAGCCCAGCTTGAAAATCGGACGTGGAATTCTCGGGTGCCAAGGAAC1215490.16939505510931258RNA PCR Primer, Index 1 (100% over 24bp)
AGCGCGCGACCTATACCCGGCCGTCGGGGCTGGAATTCTCGGGTGCCAAG1207390.16826620999632486No Hit
CGAGGCGCTGTCTACGAGTCGGGTTGTTTGGAATTCTCGGGTGCCAAGGA1203130.16767252108504982RNA PCR Primer, Index 1 (100% over 22bp)
AGGCAGGGCTAGTGACTGGAGTGATGGAATTCTCGGGTGCCAAGGAACTC1138530.15866963289998734RNA PCR Primer, Index 1 (100% over 26bp)
CGTGAACCATCGAGTCTTTGAACGCAAGTTGGAATTCTCGGGTGCCAAGG1129790.1574515950867142Illumina Small RNA Adapter 2 (100% over 21bp)
AATGTGATTTCTGCCCAGTGCTCTGATGGAATTCTCGGGTGCCAAGGAAC1118790.15591859555055804RNA PCR Primer, Index 1 (100% over 24bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAG1072770.1495050829456575No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTGGAATTCTCGGGTGCCAAGGAA1063710.1482424487822416RNA PCR Primer, Index 1 (100% over 23bp)
CGAGGCGCTGTCTACGAGTCGGGTTGTTTTGGAATTCTCGGGTGCCAAGG1058960.14758047170981056Illumina Small RNA Adapter 2 (100% over 21bp)
CGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAA1038240.14469285803806914RNA PCR Primer, Index 1 (100% over 23bp)
AGCGTGCTCCGGGCGTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT987560.13762991108421518RNA PCR Primer, Index 1 (100% over 30bp)
AGCGTGCTCCGGGCGTCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC975740.13598263340081831RNA PCR Primer, Index 1 (100% over 31bp)
TGCCGAAGGCAGGGCTAGTGACTGGAGTGTGGAATTCTCGGGTGCCAAGG955370.1331437969870455Illumina Small RNA Adapter 2 (100% over 21bp)
GCGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAGGAA951140.13255428898359636RNA PCR Primer, Index 1 (100% over 23bp)
ATCGTTCGTCTGACTTGGGTATAGGGTGGAATTCTCGGGTGCCAAGGAAC944080.13157038200857252RNA PCR Primer, Index 1 (100% over 24bp)
GCGGATTACGGTGGCGGCCTAAGCCCGGTGGAATTCTCGGGTGCCAAGGA939140.13088192585324418RNA PCR Primer, Index 1 (100% over 22bp)
CCATCGAGTCTTTGAACGCAAGTTGCGCCCTGGAATTCTCGGGTGCCAAG924790.1288820582765314No Hit
ACCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAACT922460.1285573411020547RNA PCR Primer, Index 1 (100% over 25bp)
GATGTCGGCTCTTCCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC916880.12777969224644095RNA PCR Primer, Index 1 (100% over 31bp)
GGCGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAGGA906730.1263651517653514RNA PCR Primer, Index 1 (100% over 22bp)
CCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAACTC894230.12462310683790123RNA PCR Primer, Index 1 (100% over 26bp)
GAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGACC876690.12217866939570315RNA PCR Primer, Index 1 (95% over 24bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG873480.12173131225833396Illumina Small RNA Adapter 2 (100% over 21bp)
CTGCGGAAAAATAGCTCGACGCCAGGATGGAATTCTCGGGTGCCAAGGAA868550.12104424973894759RNA PCR Primer, Index 1 (100% over 23bp)
AGGCTCCCAAAGGCACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA857860.11955445291699221RNA PCR Primer, Index 1 (100% over 32bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCATGGAATTCTCGGGTGCCAA839450.1169887691478436No Hit
CCGACGCGGATTACGGTGGCGGCCTAAGCCCTGGAATTCTCGGGTGCCAA838400.11684243737393779No Hit
CCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAAC834080.116240386647011RNA PCR Primer, Index 1 (100% over 24bp)
AAAACGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG829390.11558677139023171RNA PCR Primer, Index 1 (100% over 29bp)
ACGGAGGTGTGCGAAGGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC820670.11437152084884247RNA PCR Primer, Index 1 (100% over 27bp)
ACCCCTGTTTTTGGTCCCAAGGCTTGGAATTCTCGGGTGCCAAGGAACTC809140.11276465860776243RNA PCR Primer, Index 1 (100% over 26bp)
ATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCCAAGG789990.11009584577890877Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCGAAGGCAGGGCTAGTGACTGGAGTGATGGAATTCTCGGGTGCCAAG781200.10887083978592582No Hit
CATCGAGTCTTTGAACGCAAGTTGCGCCCCTGGAATTCTCGGGTGCCAAG770020.10731275480281438No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAC764440.10653510594720063RNA PCR Primer, Index 1 (95% over 22bp)
CGGATTACGGTGGCGGCCTAAGCCCGGGCTGGAATTCTCGGGTGCCAAGG762040.1062006333211302Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGAGAACTGCGAGGAGCCAACCCTGGAATTCTCGGGTGCCAAGGAACT748040.10424954300238601RNA PCR Primer, Index 1 (100% over 25bp)
AGAGCCCAGCTTGAAAATCGGACGTGGAATTCTCGGGTGCCAAGGAACTC742570.10348722414213381RNA PCR Primer, Index 1 (100% over 26bp)
TGGAGACGTCGCTGCCGTGATCGTGGTGGAATTCTCGGGTGCCAAGGAAC741700.10336597781518328RNA PCR Primer, Index 1 (100% over 24bp)
CGGGAAGAGCCCAGCTTGAAAATCGGACGTGGAATTCTCGGGTGCCAAGG734590.10237510266044962Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA731870.10199603368423646RNA PCR Primer, Index 1 (100% over 32bp)
GACATTGTCAGGTGGGGAGTTTGGCTTGGAATTCTCGGGTGCCAAGGAAC728410.10151383564831827RNA PCR Primer, Index 1 (100% over 24bp)
TTAGTTGGTGGAGCGATTTGTCTGGTTTGGAATTCTCGGGTGCCAAGGAA725100.10105254215152944RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph