FastQCFastQC Report
Thu 14 Sept 2023
SRR13780866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13780866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94965691
Total Bases11.9 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAAC80438678.470287443072468RNA PCR Primer, Index 1 (100% over 24bp)
GGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAA48875975.146697663685719RNA PCR Primer, Index 1 (100% over 23bp)
AGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAACT30830533.2464914091974544RNA PCR Primer, Index 1 (100% over 25bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGA29369793.0926737530925776RNA PCR Primer, Index 1 (100% over 22bp)
GAGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGGAA23399142.4639572200869893RNA PCR Primer, Index 1 (100% over 23bp)
ACTTTAAATAGGTAGGACGTGTCGGCTTGGAATTCTCGGGTGCCAAGGAA22196612.337329383513884RNA PCR Primer, Index 1 (100% over 23bp)
GGAGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGGA11601871.2216906840597832RNA PCR Primer, Index 1 (100% over 22bp)
AACGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC10716181.128426475620548RNA PCR Primer, Index 1 (100% over 31bp)
AGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGGAAC10435471.0988673793780956RNA PCR Primer, Index 1 (100% over 24bp)
CGGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGGA9477550.9979972661916396RNA PCR Primer, Index 1 (100% over 22bp)
AAACGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT7398420.7790624089704143RNA PCR Primer, Index 1 (100% over 30bp)
CGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAGGAAC6055850.6376881941500325RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGATTACGGTGGCGGCCTAAGCCCGTGGAATTCTCGGGTGCCAAGGA5705370.6007822340807271RNA PCR Primer, Index 1 (100% over 22bp)
GGAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGAAC5485550.5776349271233123RNA PCR Primer, Index 1 (100% over 24bp)
TGGAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGAA5457600.5746917589427112RNA PCR Primer, Index 1 (100% over 23bp)
GAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGAACT4996930.5261826610622988RNA PCR Primer, Index 1 (100% over 25bp)
CCCGCGACGTCGCGAGAAGTCCACTAAACCTTGGAATTCTCGGGTGCCAA4844180.5100979047264553No Hit
GCGGATTACGGTGGCGGCCTAAGCCCGTGGAATTCTCGGGTGCCAAGGAA4810940.5065976932658764RNA PCR Primer, Index 1 (100% over 23bp)
TACCGTGCGCTGGATTATGACTGTGGAATTCTCGGGTGCCAAGGAACTCC4796340.5050602959336125RNA PCR Primer, Index 1 (100% over 27bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAAC4687240.4935719364164896RNA PCR Primer, Index 1 (100% over 24bp)
AACTTTAAATAGGTAGGACGTGTCGGCTTGGAATTCTCGGGTGCCAAGGA4552990.47943525204276144RNA PCR Primer, Index 1 (100% over 22bp)
AGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGAACTC4139450.4358889991123215RNA PCR Primer, Index 1 (100% over 26bp)
AGCCCAGCTTGAAAATCGGACGTGGAATTCTCGGGTGCCAAGGAACTCCA3856130.4060550667714301RNA PCR Primer, Index 1 (100% over 28bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAA3705260.39016827666741244RNA PCR Primer, Index 1 (100% over 23bp)
GTGAACCATCGAGTCTTTGAACGCAAGTGGAATTCTCGGGTGCCAAGGAA3560190.37489223344881467RNA PCR Primer, Index 1 (100% over 23bp)
AAAACGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG3533380.37206910862155473RNA PCR Primer, Index 1 (100% over 29bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTGGAATTCTCGGGTGCCAAGGA3495510.36808135266451125RNA PCR Primer, Index 1 (100% over 22bp)
AACGGAGGTGTGCGAAGGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTC3387350.35669197626330124RNA PCR Primer, Index 1 (100% over 26bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTTGGAATTCTCGGGTGCCAAGGA3334460.35112259647539446RNA PCR Primer, Index 1 (100% over 22bp)
CGGAGGTGTGCGAAGGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA3049820.32114966656747646RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGTTGTGGGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAT2751090.2896930429327366RNA PCR Primer, Index 8 (97% over 36bp)
GCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGGAAC2645120.2785342761313662RNA PCR Primer, Index 1 (100% over 24bp)
TGCGGAAAAATAGCTCGACGCCAGGATGGAATTCTCGGGTGCCAAGGAAC2616820.2755542525352656RNA PCR Primer, Index 1 (100% over 24bp)
ACGGAGGTGTGCGAAGGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC2524220.26580336260597526RNA PCR Primer, Index 1 (100% over 27bp)
CGTGAACCATCGAGTCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACT2474230.2605393562607784RNA PCR Primer, Index 1 (100% over 25bp)
ACGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2437190.2566390002890623RNA PCR Primer, Index 1 (100% over 32bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGGAATTCTCGGGTGCCAAGGA2404420.2531882803864398RNA PCR Primer, Index 1 (100% over 22bp)
GGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGGAA2375590.2501524471611542RNA PCR Primer, Index 1 (100% over 23bp)
CGTGAACCATCGAGTCTTTGAACGCAATGGAATTCTCGGGTGCCAAGGAA2367220.249271076224781RNA PCR Primer, Index 1 (100% over 23bp)
GATGTCGGCTCTTCCTATCATTGTGAAGTGGAATTCTCGGGTGCCAAGGA2334140.24578771295414467RNA PCR Primer, Index 1 (100% over 22bp)
GCGGCGGGTAGAATCCTTTGCAGACGTGGAATTCTCGGGTGCCAAGGAAC2250420.23697189756666961RNA PCR Primer, Index 1 (100% over 24bp)
CCGGAGGTAGGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAA2173530.2288752892873701RNA PCR Primer, Index 7 (97% over 35bp)
AGCGTGCTCCGGGCGTCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2161350.2275927208279883RNA PCR Primer, Index 1 (100% over 31bp)
AGGCAGGGCTAGTGACTGGAGTGATGGAATTCTCGGGTGCCAAGGAACTC2121770.22342489984093308RNA PCR Primer, Index 1 (100% over 26bp)
GAAGAGCCCAGCTTGAAAATCGGACGTGGAATTCTCGGGTGCCAAGGAAC2115220.22273517706515714RNA PCR Primer, Index 1 (100% over 24bp)
GACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGGAACTC2060590.21698257321162442RNA PCR Primer, Index 1 (100% over 26bp)
AACGGGGTTGTGGGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2007440.21138581511506085RNA PCR Primer, Index 1 (100% over 34bp)
CGTGAACCATCGAGTCTTTGAACGCAAGTGGAATTCTCGGGTGCCAAGGA1977030.20818360601409197RNA PCR Primer, Index 1 (100% over 22bp)
AGGCTCCCAAAGGCACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1959640.20635241836970364RNA PCR Primer, Index 1 (100% over 32bp)
ACTTTAAATAGGTAGGACGTGTCGGCTGTGGAATTCTCGGGTGCCAAGGA1948790.20520990048921986RNA PCR Primer, Index 1 (100% over 22bp)
GCCCATTAAAGCGGTACGTGAGCTGGTGGAATTCTCGGGTGCCAAGGAAC1943590.20466233431608474RNA PCR Primer, Index 1 (100% over 24bp)
AGCGTGCTCCGGGCGTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1943450.20464759214988493RNA PCR Primer, Index 1 (100% over 30bp)
CGCTTGTGGAGACGTCGCTGCCGTGATCTGGAATTCTCGGGTGCCAAGGA1821040.1917576738319105RNA PCR Primer, Index 1 (100% over 22bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCTGGAATTCTCGGGTGCCAAG1592890.1677332079856082No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCTTGGAATTCTCGGGTGCCAAGG1592160.167656338118995Illumina Small RNA Adapter 2 (100% over 21bp)
GTGAACCATCGAGTCTTTGAACGCAATGGAATTCTCGGGTGCCAAGGAAC1576350.16599152635029002RNA PCR Primer, Index 1 (100% over 24bp)
GTGAACCATCGAGTCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACTC1539570.16211854868723063RNA PCR Primer, Index 1 (100% over 26bp)
AGGCCTACGGGTCCTGAACTTCTTTTCCCTGGAATTCTCGGGTGCCAAGG1539510.16211223061600216Illumina Small RNA Adapter 2 (100% over 21bp)
CCGACCCTCAGTAGGAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1536970.16184476560066308RNA PCR Primer, Index 1 (100% over 30bp)
ACGGGGTTGTGGGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAA1533980.1615299150511104RNA PCR Primer, Index 7 (97% over 35bp)
GCGTTCGAATTGTAGTCTGGAGAAGCGTGGAATTCTCGGGTGCCAAGGAA1442590.1519064395582611RNA PCR Primer, Index 1 (100% over 23bp)
AGGCCTACGGGTCCTGAACTTCTTTTCCCAGATGGAATTCTCGGGTGCCA1435560.1511661722126573No Hit
CGGCGTTCGAATTGTAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1402340.14766806677582117RNA PCR Primer, Index 1 (100% over 29bp)
GGCGTTCGAATTGTAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1331310.14018852345317007RNA PCR Primer, Index 1 (100% over 30bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAGG1296950.13657037466299277Illumina Small RNA Adapter 2 (100% over 21bp)
ACTTTAAATAGGTAGGACGTGTCGGCTGGAATTCTCGGGTGCCAAGGAAC1283260.13512880141102748RNA PCR Primer, Index 1 (100% over 24bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTGGAATTCTCGGGTGCCAAGGAA1280430.13483079905141743RNA PCR Primer, Index 1 (100% over 23bp)
CCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAACT1249070.13152855382266423RNA PCR Primer, Index 1 (100% over 25bp)
GCGTTCGAATTGTAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1242260.13081145273823153RNA PCR Primer, Index 1 (100% over 31bp)
ACCGTGCGCTGGATTATGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA1241090.13068825034927614RNA PCR Primer, Index 1 (100% over 28bp)
TGCGGAAAAATAGCTCGACGCCAGGTGGAATTCTCGGGTGCCAAGGAACT1240480.1306240166251199RNA PCR Primer, Index 1 (100% over 25bp)
TACCCCGTAGGCTGAGGAGCAAAAGGTGGAATTCTCGGGTGCCAAGGAAC1240080.13058189615026336RNA PCR Primer, Index 1 (100% over 24bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCTGGAATTCTCGGGTGCCAAGG1226210.12912136868461266Illumina Small RNA Adapter 2 (100% over 21bp)
TGGTCGGCTTGTCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1225090.1290034313550143RNA PCR Primer, Index 1 (100% over 34bp)
CGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1198060.12615714026658323RNA PCR Primer, Index 1 (100% over 33bp)
AGCGCGCGACCTATACCCGGCCGTCGGGGCTGGAATTCTCGGGTGCCAAG1188480.12514835489376894No Hit
TAGGTAGGACGTGTCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1186970.12498935010118548RNA PCR Primer, Index 1 (100% over 31bp)
GCGGATTACGGTGGCGGCCTAAGCCCGGTGGAATTCTCGGGTGCCAAGGA1178250.12407112374931278RNA PCR Primer, Index 1 (100% over 22bp)
TAACGGAGGTGTGCGAAGGTAGGCTTGGAATTCTCGGGTGCCAAGGAACT1172990.12351723950494921RNA PCR Primer, Index 1 (100% over 25bp)
CGCGGGTGCACCGCCGACCGACCTTGTGGAATTCTCGGGTGCCAAGGAAC1151440.1212479989220528RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGATTACGGTGGCGGCCTAAGCCCTGGAATTCTCGGGTGCCAAGGAA1144510.12051826169516315RNA PCR Primer, Index 1 (100% over 23bp)
GACATTGTCAGGTGGGGAGTTTGGCTTGGAATTCTCGGGTGCCAAGGAAC1137130.11974113893405988RNA PCR Primer, Index 1 (100% over 24bp)
AGAGCCCAGCTTGAAAATCGGACGTGGAATTCTCGGGTGCCAAGGAACTC1088500.11462034220337532RNA PCR Primer, Index 1 (100% over 26bp)
TGTGGAGACGTCGCTGCCGTGATCGTGTGGAATTCTCGGGTGCCAAGGAA1087790.11454557836050497RNA PCR Primer, Index 1 (100% over 23bp)
GAAGGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1069580.1126280437426607RNA PCR Primer, Index 1 (100% over 33bp)
CTGCGGAAAAATAGCTCGACGCCAGGATGGAATTCTCGGGTGCCAAGGAA1064970.11214260526993902RNA PCR Primer, Index 1 (100% over 23bp)
CGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAA1064510.11209416672385399RNA PCR Primer, Index 1 (100% over 23bp)
TGGAGACGTCGCTGCCGTGATCGTGGTGGAATTCTCGGGTGCCAAGGAAC1058370.11144761743480602RNA PCR Primer, Index 1 (100% over 24bp)
CGGGAAGAGCCCAGCTTGAAAATCGGACGTGGAATTCTCGGGTGCCAAGG1036260.10911940818711043Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCGAAGGCAGGGCTAGTGACTGGAGTGTGGAATTCTCGGGTGCCAAGG1006950.10603303039199705Illumina Small RNA Adapter 2 (100% over 21bp)
CGATGCCGACCAGGGATCAGCGGATGTGGAATTCTCGGGTGCCAAGGAAC995910.10487050528595637RNA PCR Primer, Index 1 (100% over 24bp)
GATGTCGGCTCTTCCTATCATTGTGAATGGAATTCTCGGGTGCCAAGGAA981230.10332468385872114RNA PCR Primer, Index 1 (100% over 23bp)
TCGGCGTTCGAATTGTAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA979450.1031372477456095RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATAACCGTAGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAA977860.10296981885805476RNA PCR Primer, Index 1 (100% over 23bp)
TCGGCGTTCGAATTGTAGTCTGGAGAAGCTGGAATTCTCGGGTGCCAAGG968840.10202000215003965Illumina Small RNA Adapter 2 (100% over 21bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGA964360.10154825283164634RNA PCR Primer, Index 1 (100% over 22bp)
ACGATGCCGACCAGGGATCAGCGGATGTGGAATTCTCGGGTGCCAAGGAA955080.10057105781497445RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph