FastQCFastQC Report
Wed 6 Sept 2023
SRR13781488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13781488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82390271
Total Bases8.2 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC80714799.796640916498504No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT59002897.1613904510643005No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC36777864.463859573905273No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT19037992.3107085058623No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT17603972.1366564020647534No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC10647591.2923358390215756No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT10056171.2205530917600695No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT9515711.154955540806511No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA8580131.0414008712266525No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT8192910.9944026036763491No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT8171440.991796713473609Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC8017150.9730699878387341No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA6701740.8134140012720676No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT6299570.7646011990906062No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC5932500.7200486086518637No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC5671500.6883701110777994No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC5592700.6788058750286183No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA5282930.6412079892296992No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTG4968610.6030578537604276Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC4960060.6020201098743807No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT4261580.517243109929812No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCCAGATCGGAAGAGCACACGT3813900.4629065973092867No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC3708920.45016480161838524No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3593990.43621533906594384No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA3563760.4325462165308329Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC3451730.41894873728476995No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG3411720.414092581392286No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT3348410.4064084216933817No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC3327470.4038668594742212No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC3259060.39556369465030644No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2834050.34397872049722955No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCAGATCGGAAGAGCACACGTC2829350.3434082647937886No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT2606580.3163698781862242No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGT2546130.30903284685154153No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC2488460.30203323399676646No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT2413350.2929168663615635No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2133060.2588970729323126No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG1824550.22145211781133722No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1810530.2197504605853281No Hit
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA1790100.21727079887866857Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCAGATCGGAAGAGCACACGT1753380.21281396197859334No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCCAGATCGGAAGAGCACACGT1744810.2117737906214679No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC1735600.21065594018983141No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCGGGAGATCGGAAGAGCACACGT1643840.1995187028818002No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCAGATCGGAAGAGCACACGTC1604140.19470017279103255No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1547820.18786441423405442No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGT1517450.18417829940139413No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGCAGATCGGAAGAGCACACGT1481840.17985618714617407No Hit
ACCCAAATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCAC1470100.17843126162311082No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT1468980.17829532324271635No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1416240.17189408200878475No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCAGATCGGAAGAGCACACGT1363840.16553410778318717No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGA1363740.16552197042779482No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCCAGATCGGAAGAGCACACGTC1318390.16001767975735873No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1311890.1592287516568552No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCGGGAGATCGGAAGAGCACACGT1267690.15386404057343128No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC1223450.14849447454785045No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1221650.14827600215078793No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCCGCCAGATCGGAAGAGCACACGT1216750.1476812717365622No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTC1186900.1440582711519422No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCAGATCGGAAGAG1176110.14274865050510635No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGTC1175190.14263698683549664No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCCGGAGATCGGAAGAGCACACGT1133520.13757935084349948No Hit
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1126740.13675643814789734Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTCT1112200.13499166667384793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGCAGATCGGAAGAGCACACGT1098060.13327544462136798No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAGAGCACA1089050.13218186890051617No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACA1087380.13197917506546375No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA1059330.12857464687790626No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGAGATCGGAAGAGCACACGTC1028960.12488853204524597No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCAGATCGGAAGAGC1022320.12408261164719314No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG1014690.12315653143075593No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAGATCGGAAGAGCACA1011220.122735365198641No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG1005120.12199498651970693No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTTAGATCGGAAGAGCACACG1004840.1219610019246083No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC979860.11892909054759633No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCACA976910.11857103856352166No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGCGAGATCGGAAGAGCACA956380.11607923950146977No Hit
GCATTGGTGGTTCAGTGGTAGGATTCTCGCAGATCGGAAGAGCACACGTC942670.11441520807717698No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT934850.11346606688549428No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGCAGATCGGAAGAGCACA910020.11045236154157084No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA903600.10967314332538122No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAGAGCACAC903150.10961852522611559No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCCAGATCGGAAGAGCACACGTCTG885220.10744229740426513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACAGGGAGATCGGAAGAGCACA879670.10676867417998903No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCCGGAGATCGGAAGAGCACACGT876270.10635600409664875No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT848870.10303036871914162No Hit
GGTCCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC848720.10301216268605307No Hit
GCATTGGTGGTTCAGTGGTAGAATTCGCGCAGATCGGAAGAGCACACGTC846750.10277305678482354No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGCAGATCGGAAGAGCACACGT841240.10210428850270441No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG838140.10172803048554119Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA837210.10161515308039222Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)