FastQCFastQC Report
Wed 6 Sept 2023
SRR13781496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13781496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88379175
Total Bases8.8 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC68905707.796599142275315No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT41108004.651321988466174No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT37531524.246647470968132No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC36408544.119583601001028No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC17834282.017927865925429No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT14864301.6818781121231332No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT12332791.3954407245824596No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC11463991.2971370235126092No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA10612431.2007840082236567No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC9310861.0535128892072143No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT8259220.9345210565724336No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA7692100.8703520936917548No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT7370510.8339645623530656No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT6940000.7852528607559417No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGT6918880.7828631575255143No Hit
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG6550710.74120515381593Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC6205150.7021054450892985No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT6099030.6900980915470188Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTG5214340.589996455612988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC5120630.5793932790162388No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC4667540.5281266769009781No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA4504630.5096936014621092Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT3701010.41876494094904143No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG3631610.41091241234148207No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC3570050.403946970539157No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT3498820.39588737957782477No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC3191640.3611303228390625No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC3141740.3554841963618692No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGTC3037770.3437201127980658No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2878220.32566721741858307No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCAGATCGGAAGAGCACACGT2828830.32007879684326085No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2640510.2987706097052841No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCAGATCGGAAGAGCACACGTC2637750.29845831894221686No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCCAGATCGGAAGAGCACACGT2604420.2946870685316988No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGT2584400.29242182901118957No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG2570560.29085584924276564No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGAGATCGGAAGAGCACACGTC2489560.28169079423970633No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA2330990.26374878471087787Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2285430.25859372414372506No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2277060.25764666846007556No Hit
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1994360.22565949501112675Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCCCGCAGATCGGAAGAGCACACGTC1982290.22429378866684374No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGT1942040.21973954837211368No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCGGGAGATCGGAAGAGCACACGT1867000.21124886037915605No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1786630.2021550891372317Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC1777180.20108583272020814No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC1713300.19385788563878312No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1699540.19230095777653503No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA1681780.1902914346054939No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1668150.18874921609078157Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA1624950.18386118675581664Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCATATGGTCTAGCGGTTAGGATTCCCGGAGATCGGAAGAGCACACGT1587160.1795852925759943No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCGGGAGATCGGAAGAGCACACGT1586470.1795072198852275No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC1524850.17253498915327053No Hit
ACCCAAATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCAC1491490.1687603442779365No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCCAGATCGGAAGAGCACACGTC1431480.1619702831577688No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG1418960.16055365984124656No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGCGAGATCGGAAGAGCACA1418400.16049029649801552No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAGATCGGAAGAGCACA1417270.16036243832328148No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1402630.1587059394930989No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAGAGCACAC1354930.15330874043574177No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCCGGAGATCGGAAGAGCACACGT1348530.15258458794167293No Hit
AATGGATTTTTGGAGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1341220.15175747001485362Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT1322000.149582749556103No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1296100.146652194931668No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAGAGCACA1276690.14445597619574974No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGAGATCGGAAGAGCACACGT1244860.14085444902602903No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGCGAGATCGGAAGAGCACAC1241870.14051613403270624No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT1233120.13952608179472145No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCCAGATCGGAAGAGCACACGTC1224200.13851679425611294No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1222580.13833349315605176No Hit
GCATTGGTGGTTCAGTGGTAGGATTCTCGCAGATCGGAAGAGCACACGTC1177650.13324971635003383No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCACA1174390.13288085117336748No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT1154370.13061561165285826Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCCCCAGATCGGAAGAGCACACGT1146670.12974436568343164No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTG1128640.12770429232904695Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1128200.12765450684507973No Hit
TCCCGTGGATCGCCCCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1102900.1247918415169637Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGCAGATCGGAAGAGCACA1076700.12182734224436922No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGGAGATCGGAAGAGCACACGTCT1071080.12119144583551497Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT1061550.12011313751231555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCCCGCAGATCGGAAGAGCACACGTC1061350.1200905077468759No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGCAGATCGGAAGAGCACACGT1055500.11942858710776605No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCCAGATCGGAAGAGCACACGTCTG1044460.11817942405549724Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTCT1043240.11804138248631536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCCAGATCGGAAGAGCACACGT1040480.11772909172324815No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGGAGATCGGAAGAGCACACG1019720.11538012207061223No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGTC997610.11287840150125864No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTAGATCGGAAGAGCACACGT990180.11203770571517555No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACAGGGAGATCGGAAGAGCACA961790.10882541051101687No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCAGATCGGAAGAGCACACGT944890.10691319533136624No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGCAGATCGGAAGAGCACAC938550.10619583176692926No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGCAGATCGGAAGAGCACACGT899170.10174003095186168No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGGAGATCGGAAGAGCACACGTC895180.10128856713134061No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACA894470.10120823146402985No Hit
TCCCTGTAGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGTC887080.10037206163103468No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG886570.10031435572916357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)