FastQCFastQC Report
Thu 14 Sept 2023
SRR13781497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13781497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95175564
Total Bases9.5 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1086693911.417782614873708No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC58934566.192194458653274No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT21829942.2936496599064022No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT20600132.164434770252583No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC16175141.6995055579602343No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA15216001.5987296907428887No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT10465441.0995931686835079No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA10137051.0650895643759988Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA9905581.040769246190125No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT9170930.96358031563648Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC8946880.9400396093266125No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC8866080.9315500352590502No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA8325020.8747014097021795No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC7860470.8258916122629963No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT7476230.7855199050882431No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC7418840.7794899959825822No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC6722090.7062831799977566No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC6523990.6854690138741915No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT6122560.6432911708303615No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT5717650.6007476877153047No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT4919410.5168774203428939No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC4796910.5040064695597707No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCAGATCGGAAGAGCACACGTC4571820.48035649150447907No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCAGATCGGAAGAGCACACGTC4393210.46159011991775534No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGGAAGA4335510.4555276394264393No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGT4209860.44232572133746434No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTG4079580.4286373338433802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCAAATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCAC4015300.421883499424285No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA3542300.37218586905353146Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG3488710.36655522209461244No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC3474820.36509581387928525No Hit
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC3430460.3604349536610048No Hit
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3232700.3396565109926746Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC3118610.3276691903816824No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCAGATCGGAAGAG3078580.3234632788727157No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT3033160.3186910455292915No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG2963350.311356179617701No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCACA2938000.3086926808229894No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAGATCGGAAGAG2891480.3038048715949821No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2862050.300712691337453No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCCAGATCGGAAGAGCACACGTC2522320.2650176047288777No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG2451950.25762390018513576No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCCGCAGATCGGAAGAGCACACGTC2417210.253973803612028No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT2302360.24190663057168751No Hit
GCATTGGTGGTTCAGTGGTAGGATTCTCGCAGATCGGAAGAGCACACGTC2278470.23939653249651352No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGT2199080.23105510569918974No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2191090.23021560450117218No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1976550.20767410424801896No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCAGATCGGAAGAGC1914680.20117348608514682No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACA1914140.20111674883271508No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC1894020.19900276083470333No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAGATCGGAAGAGCACA1851840.19457095100586952No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGTC1843760.19372199359911332No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG1785730.18762484034242236No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1767510.18571048341778149No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCGGGAGATCGGAAGAGCACACGT1704060.17904385625705355No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCAGATCGGAAGAGCAC1696310.1782295716156723No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1683260.17685842134857221No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1629230.17118154403581995No Hit
TGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCA1560100.16391812503469902No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCGGGAGATCGGAAGAGCACACGT1559200.16382356294731282No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCGAGATCGGAAGAGCA1515970.1592814306831951No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCAGATCGGAAGAGCACACGT1467240.15416141899616168No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGAGATCGGAAGAGCACACGTC1449630.15231115415297145No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTCTGA1440280.1513287591340147Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1432960.15055965415660683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGA1391890.14624447090221604No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT1381550.14515805758713443Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTCT1358550.1427414709094868Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCCCGCCAGATCGGAAGAGCACACGT1354690.14233590462358595No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCCGGAGATCGGAAGAGCACACGT1330300.1397732720554196No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG1323630.1390724619189018No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1304150.13702571807192024No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCCGGAGATCGGAAGAGCACACGT1226570.12887446613922876No Hit
GCATGGGTGGTTCAGTGGTAGGATTCTCGCAGATCGGAAGAGCACACGTC1205550.1266659160538308No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTC1200080.1260911887004946No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCAGATCGGAAGAGCACACG1193340.12538302373495785No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC1190650.12510038816265906No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGCGAGATCGGAAGAGCACA1186070.12461917220684922No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACAGATCGGAAGAGCACACGTC1126040.1183118809781889No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC1122790.11797040677373868No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCAGATCGGAAGAGCACACGT1087260.11423730570170301No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAGAGCACAC1060840.11146138309198778No Hit
TCCCGTGGATCGCCCCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1059900.11136261824516216Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG1052260.11055989119223922Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGT1040550.10932953336635862No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCCAGATCGGAAGAGCACACGT1031140.10834083420824278No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCAGATCGGAAGAGCA998270.10488721663892635No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGCAGATCGGAAGAGCACA995570.10460353037676771No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT955620.10040602438667975No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)