FastQCFastQC Report
Wed 6 Sept 2023
SRR13781498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13781498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76540367
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT950299112.41565904694447No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC46160806.030909154119943No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT31575044.125279409752503No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC20880322.728014094837042No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT14111331.8436454583501016No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT13022791.701427692396615No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC8041581.0506325374687582No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT7344130.959510685387751No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT6171630.806323544280889No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA6159550.8047452921149438No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCCAGATCGGAAGAGCACACGT6119540.7995179850653185No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC6020680.7866019247072593No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC5596190.7311423003759572No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC5205920.6801535195147418No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT4539210.593047848856016No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT4533640.5923201282794999No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA4397770.5745687109130271No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT4337460.5666892085845369No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG4169330.5447230217749021No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT3872070.5058859986913833Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTG3736160.4881293553243611Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGGCAGATCGGAAGAGCACACGT3676630.48035176000658586No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC3250460.42467264365220514No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT3225700.4214377493120721No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC3156920.4124516413672279No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC3123880.4081349649133509No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCCAGATCGGAAGAGCACACGT2951730.3856435650484926No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACCAGATCGGAAGAGCACACGT2670910.34895442819081335No Hit
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2430700.31757098839100156No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT2347560.3067087462488911No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC2333630.30488879155753196No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCAGATCGGAAGAGCACACGT2187190.2857564035458571No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2085170.27242748914438836No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCCGCCAGATCGGAAGAGCACACGT2013780.2631003846636899No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG1873680.24479631773910884No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA1799460.23509947371953416No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1730400.22607678377084342No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG1714590.22401120705365837No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTTAGATCGGAAGAGCACACG1696570.22165689380611409No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGT1633370.21339981293792332No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC1554090.20304187984883848No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT1490700.1947599754780376No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC1484960.19401004439918612No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC1473390.19249842374024678No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1404270.18346789479073178No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA1378690.18012586743933434No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1363390.17812692222915524No Hit
GCATTGGTGGTTCAGTGGTAGAATTCGCGCAGATCGGAAGAGCACACGTC1351270.17654344406266043No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC1303240.17026832390286292No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1299560.16978753185231005No Hit
ACCCAAATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCAC1216800.1589749367154197No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGGCAGATCGGAAGAGCACACGT1216560.15894358071212278No Hit
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA1216120.15888609470607842Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTCCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC1189510.15540949784053165No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACA1167660.15255479504037392No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1165110.1522216375053441Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1119600.1462757553801643No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1117140.1459543563463708No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAGATCGGAAGAGCACA1101540.14391621613207053No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC1086860.1419982739304085No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCGGGAGATCGGAAGAGCACACGT1083480.1415566768839768No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACA1083220.1415227078804051No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCAGATCGGAAGAGCACACGTC1078080.14085116680979595No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACG1051330.13735627894232594No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGACAGATCGGAAGAGCACACGT1009630.1319081733694849No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTCAGATCGGAAGAGCACACGT1008150.13171481134915383No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC992070.12961395912825974No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCAGATCGGAAGAGCACACGT987230.1289816130617717No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCAGATCGGAAGAGCACACG987010.12895287005874953No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCCCAGATCGGAAGAGCACACGT982170.1283205239922615No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC975580.12745954040173338No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCA929590.12145094626995975No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC915810.11965058908066119No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGGAGATCGGAAGAGCACAC909650.11884578499604007No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGT906770.11846951295647694No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG899320.11749617035413484No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCACCAGATCGGAAGAGCACACGT885640.11570887816620999No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT880350.11501773959354024No Hit
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA880320.11501382009312812Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTTCCATGGTGTAATGGTTAGCACTCGGGAGATCGGAAGAGCACACGTC859610.1123080583086308No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCCAGATCGGAAGAGCACACGTCTG848600.11086960165738427Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCGCGCCAGATCGGAAGAGCACACGT848120.11080688965079041No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC845670.11048679711713429No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGAGATCGGAAGAGCACACGTC842850.11011836407839538No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG806290.1053417995761635No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGA780540.10197756172243073No Hit
TCCCGTGGATCGCCCCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT777920.10163525868643927Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT772220.10089055360813727No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCGGGAGATCGGAAGAGCACACGT771710.1008239221011313No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)