FastQCFastQC Report
Thu 14 Sept 2023
SRR13781499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13781499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123883739
Total Bases12.3 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1954913615.780227621318405No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT54724304.417391696580937No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC37556723.031610145379936No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT32755262.6440322405832455No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT23205301.8731514069009494No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG16801761.356252251960203No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC14138651.1412837644495053No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC13509521.0904998597112088No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC13432411.084275475411668No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT12411301.0018506141471883No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT11072750.8938017280863633No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT10754900.8681446077438784No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG8387910.677079176630276No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA8218670.6634179809506718No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT6762570.5458803596491385No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT6758110.5455203446838168No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG6714320.5419855789144368No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT6569720.5303133448369685No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC5356240.4323602147655553No Hit
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC4839690.39066386267208164Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGGCAGATCGGAAGAGCACACGT4568190.3687481534602374No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCCAGATCGGAAGAGCACACGT4552310.3674663064536662No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG4504000.36356668246831003No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC4358430.35181614917192644No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT4312010.3480690875821887No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG3702480.29886731139104544No Hit
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC3611470.2915209073565337No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT3203230.2585674299029673No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA2962670.2391492236119867Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT2892420.2334785843039497Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT2854810.2304426733519885No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA2751220.22208080109690584No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC2739040.22109762121403195Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT2707080.21851778303204106No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTG2547570.20564200116691667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2464300.19892037646684202No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2401280.19383334886267842No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCAGATCGGAAGAGCACACGT2397040.19349109248309013No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTTAGATCGGAAGAGCACACG2322830.18750079863185273No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGACAGATCGGAAGAGCACACGT2081760.16804142471030845No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC2070460.16712927917036796No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC2042170.16484568648674705No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACCAGATCGGAAGAGCACACGT2040460.16470765384309236No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC2030170.1638770363558368No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTCAGATCGGAAGAGCACACGT1996370.16114867182043965No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG1943170.1568543229067376No Hit
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA1925740.15544735859159045Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC1916910.154734593536929No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT1906790.1539176985931947No Hit
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA1882820.15198281995670151Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCAGATCGGAAGAGCACACG1833730.1480202337128362No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACG1810550.14614912454329457No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1768490.1427540058344542No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG1749000.1411807565801675No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA1716470.13855490751695831No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG1648850.1330965640292791No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC1647020.1329488448843153No Hit
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1636670.1321133841464052Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCCCCAGATCGGAAGAGCACACGT1616080.13045134196345173No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGGAGATCGGAAGAGCACACG1594170.1286827482660981No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG1580310.12756395736489679No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCAGATCGGAAGAGCACACGT1563020.1261682939679436No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC1540940.12438597772706876No Hit
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1527790.12332449862527964No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1523520.12297982062036406No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC1427750.11524918536725794No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGAGATCGGAAGAGCACACGT1392880.11243444952852126No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC1326040.10703906829935121No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGATCGGAAGAGCACACG1314370.10609705604704102No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCCGCCAGATCGGAAGAGCACACGT1293660.10442532736277843No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGGCAGATCGGAAGAGCACACGT1293360.10440111110950566No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGT1283440.10360036033461988No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1273140.10276893563892192No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGT1241730.10023349392126435No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)