FastQCFastQC Report
Wed 6 Sept 2023
SRR13781500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13781500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42891311
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT582241713.574817053272165No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT14124293.2930422667658727No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC10072022.3482658294123953No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT7837721.8273444707717141No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC7601921.7723683008896602No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG7115441.658946726995591No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG5041921.1755108161650736No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT4746151.1065527934084365No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC4690321.0935361709974312No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT4424441.0315469256698635No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC3983330.9287032518078079No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA3922550.9145325494946984No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3767770.8784459864143579No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC3132040.7302271548659354No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT2844500.6631879356637058No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2555090.5957127307206814No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2504840.5839970711084117No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG2356490.5494096461635318No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT2336660.544786332131466No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2301490.536586536140152No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1911000.44554478644870515No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1791340.41764636198693017No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC1593300.3714738400045641No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGCAGATCGGAAGAGCACACGT1493890.3482966515059425No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1455580.3393647725060211No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG1324700.3088504335994766No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1213680.28296640314864707No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG1187280.27681131033742473No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1115620.2601039637142357No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCCAGATCGGAAGAGCACACGT1013580.23631359740904165No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGATCGGAAGAGCACACG979120.2282793361107568No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT961070.2240710245485385No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTCAGATCGGAAGAGCACACGT954750.2225975326331247No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCAGATCGGAAGAGCACACG901610.21020807687599008No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTTAGATCGGAAGAGCACACG877570.20460321205849827No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACCAGATCGGAAGAGCACACGT859360.20035759690348473No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC851210.1984574451454748No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT849040.19795151516818874Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC841140.19610965027392147No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG837890.19535192104526716No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT786620.18339845102892752No Hit
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT784730.1829578023390332No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA764260.1781852739357862No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGACAGATCGGAAGAGCACACGT747910.17437331304701784No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG747400.17425440784498286No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC734800.17131674991235404No Hit
ACCCAAATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCAC714320.16654189003455735No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACG699450.16307498737914541No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC667560.15563991504013483No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG659700.15380737604406636No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC653690.15240615983969338No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGGAGATCGGAAGAGCACAC633460.14768958682563935No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC614510.14327144255394758No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC579430.13509262983358097No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAGATCGGAAGAGCACA577060.1345400703653008No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT573370.1336797562564595No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCAGATCGGAAGAGCACACGT572580.13349556976703275No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACG571520.133248433464764No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT552470.12880697444757516No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGGAGATCGGAAGAGCACACG547010.12753398934343602No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC536050.12497869323695887No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG535970.12496004144056122No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT534280.12456602224166102No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG524960.12239308796133556No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT524860.12236977321583853No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAAGATCGGAAGAGCACACG519410.12109911958624907No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG515530.12019450746096336No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT514390.11992871936229695No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCCAGATCGGAAGAGCACACGT509580.11880728010388864No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCA491860.11467590720181063No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT484620.11298791962782392No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCAGATCGGAAGAGCACACGT456550.10644347056680081No Hit
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA451370.1052357667500534Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC446500.10410033864434688No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC431160.10052385668509876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)