FastQCFastQC Report
Wed 6 Sept 2023
SRR13781501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13781501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46900775
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT33816067.21012819084546No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC12073172.5741941364508367No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG9397232.003640664786456No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG8587871.831072087827973No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT7915361.6876821331843663No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG6786951.4470869617826145No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT5903581.258738261787785No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC5027081.0718543563512544No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG4354590.9284686660295058No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC3766330.8030421672136547No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAGATCGGAAGAGCACA3587680.7649511122150967No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT3482850.7425996691952318No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT3380600.7207983236950776No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC3264610.6960673890783255No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA3109810.6630615378956958No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG3024640.6449019232624621No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2742730.5847941745099094No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2204090.4699474582243044No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC2197420.4685253068845877No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC2151340.4587003093232468No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG2098800.44749793580170055No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG2052480.43762176637806094No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGATCGGAAGAGCACACG1892300.40346881261556977No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC1870100.39873541535294466No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG1842920.39294020194762236No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG1585310.3380136042528082No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1385950.2955068439700623No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC1374640.2930953699592384No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1354410.28878200839964796No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT1319400.2813173129868323No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAAGATCGGAAGAGCACACG1178080.25118561473664347No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACG1117260.23821781196579375No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1113040.23731804005370913No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT1088710.2321304925131834No Hit
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1036410.22097929085393578No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1035720.22083217174982717No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1017620.21697296046813727No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT966610.20609680756874488No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG957970.20425462052599347No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA949310.2024081691613838No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC929720.19823126590125642No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG917890.19570891952211877No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTCAGATCGGAAGAGCACACGT910660.19416736717037192No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA909490.19391790434166598No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC873740.18629542902009616No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA866480.18474748018556197No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT794490.16939805365689586No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC729650.15557312219254374No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC717470.1529761501808872No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA714390.1523194446147212No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG707920.15093993649358672No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA689380.14698691013101597No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCCAGATCGGAAGAGCACAC683510.14573533166562813No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGAGATCGGAAGAGCACA646360.13781435381398283No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC643290.1371597804087459No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCAGATCGGAAGAGCACACG611920.13047119157412643No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC605420.12908528697020466Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGGCAGATCGGAAGAGCACACG553600.11803642903555431No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGATCGGAAGAGCACACG546780.11658229528190098No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC541420.1154394570238978No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGCAGATCGGAAGAGCACACGT531850.11339897901473911No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC527680.11250986790730004No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT525910.1121324754228475No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGAGATCGGAAGAGCACAC520170.1109086150495381No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT518000.11044593612792113No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG509940.1087274144190581No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA497690.10611551728089781No Hit
ACCCAAATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCAC497470.1060686097404574No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC496570.10587671525683744No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTAGATCGGAAGAGCAC491120.10471468755047224No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA490730.10463153327423694Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAAGATCGGAAGAGCACAC488310.10411555032939221No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT474890.10125419036252599No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGCAGATCGGAAGAGCACACG469620.1001305415528848No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCAGATCGGAAGAGCACACGTCT469390.10008150185151525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)