FastQCFastQC Report
Sat 9 Sept 2023
SRR13781503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13781503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77230095
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC57624017.461341333323493No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT37713064.883207770235166No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC27619463.5762561213993074No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT25729283.3315095624315885No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA13981151.8103240712056614No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT12920771.6730226733503308No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT12119021.5692095160571795No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC10567251.3682813675161216No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT10100791.3078826330590427No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC9239781.1963963012087453No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT7975761.0327269440753633No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC7384070.9561130282178211No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA5437650.7040843339633338No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT5367780.6950373426317811Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC5318820.6886978450563863No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT5022040.6502698203336407No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTG4888800.6330174784842619Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT4194860.543163905210786No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC4113370.5326123190706938No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA4076660.5278589907211689No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC3941590.5103696946119255No Hit
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC3911100.5064217517795362No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG3619550.46867092420383527No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGGAGATCGGAAGAGCACACGTC3596240.4656526707626088No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGACGAGATCGGAAGAGCACACGT3534600.4576713261844363No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC3429990.4441260884115189No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCGGGAGATCGGAAGAGCACACGT3428970.44399401554536483No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA3382200.43793808618259505Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT3194830.4136768186029034No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCGGGAGATCGGAAGAGCACACGT3194460.41362890981812206No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGT2831450.3666252126195106No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2643700.3423147414230165No Hit
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2623920.33975356368524473Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG2355450.3049912084142846No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2290880.29663047805392445No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCACA2217730.2871587818194449No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGCGAGATCGGAAGAGCACA2042730.26449922144987653No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCCAGATCGGAAGAGCACACGT2024790.26217629280399046No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC1899910.2460064305242665No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC1872900.24250908923522624No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1827100.2365787585785049No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGACGAGATCGGAAGAGCACACGT1801080.2332096056595554No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAGAGCACA1730920.22412506419939013No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1635420.2117594183977114No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCAGATCGGAAGAGCACACGTC1615030.2091192559066514No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC1586720.2054535864548658No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1560780.2020947921920852No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGT1495240.1936084631256766No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1476460.19117676859001662No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT1467870.19006450788387608No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGACGAGATCGGAAGAGCACACGTC1462220.1893329277919443No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCCGGAGATCGGAAGAGCACACGT1437450.18612562887563455No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAGAGCACAC1435410.18586148314332646No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG1435150.18582781751077737No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACA1396350.1808038692688388No Hit
ACCCAAATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCAC1392430.18029629511656045No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1356860.17569057761744306No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1287790.16674717284757967Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGCGAGATCGGAAGAGCACAC1280930.16585891808109263No Hit
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA1241580.16076375407799254Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1199190.1552749611404725No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT1198180.15514418310633957Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGGGAGATCGGAAGAGCACACGTC1196190.15488651153413704No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCCGGAGATCGGAAGAGCACACGT1195900.15484896140552462No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCAGATCGGAAGAGCACACGT1195790.15483471825329234No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCAGATCGGAAGAGCACACGT1189340.15399955159967108No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1180210.15281736996439016Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGTGTAGAATAAGTGGGAGGCCCCCAGATCGGAAGAGCACACGTCTG1167580.1511819971217179Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGGAGATCGGAAGAGCACAC1099640.1423849083702409No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1095570.1418579117376458No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGT1089590.14108360218901714No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCAGATCGGAAGAGCACACGTC1083940.14035202209708536No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1051850.13619690614131705No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACAGGGAGATCGGAAGAGCACA973030.12599104015086346No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA971420.12578257219546346No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGCAGATCGGAAGAGCACACGT965490.12501473680694036No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTG960670.12439062777276139Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGCAGATCGGAAGAGCACA944330.12227487225025427No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGAGATCGGAAGAGCACACGTC941370.12189160197200327No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC931090.12056051465429377No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC926150.11992086763586139No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAGATCGGAAGAGCACA844340.10932784687109345No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG843280.10919059467685492No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCAC838620.10858720295501385No Hit
TCCCGTGGATCGCCCCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT829910.10745940426461989Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT819220.10607522883404456No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCACAC814150.10541874899933762No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGCAGATCGGAAGAGCACACGT812300.10517920507543076No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACA793560.10275268986785528No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCCAGATCGGAAGAGCACACGTC790970.10241732837438565No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC785240.10167538962628493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)