FastQCFastQC Report
Wed 6 Sept 2023
SRR13784004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13784004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32094167
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG365878411.400152557316725TruSeq Adapter, Index 5 (100% over 49bp)
GGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT11955123.7250133334197457Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG9248172.8815734647358195Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA6745532.1017931389214746Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT5753291.7926279251927617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC5273191.643036879567555No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT5187741.616412103794437Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC4621921.4401121549594977Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT4115321.2822641572220896Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA4104241.2788118164898936Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC3879911.208914379986868No Hit
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG3802811.18489132308684Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3583461.1165455704147111No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG3369341.0498293973481225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA3160790.9848487421405889Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAAC3107120.9681260772401414Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2992020.9322628625943151No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2790610.8695069107105973No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2635400.821146097980982Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2491150.7762002360117338No Hit
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACA2241190.6983169246922658No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT2150630.6700999592854365Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT2084470.6494856214838042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCGGGCGGGCGTCCCGCGCGTGTAGATCGGAAGAGCACACGTCTGA2012630.6271014916822736Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT1803970.562086562333897Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1738840.5417931551237956No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG1683630.5245906522515447Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1588640.4949933737180342No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA1362610.42456624594743336Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1331940.41500999231417973Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1297720.4043476186809896No Hit
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC1297550.4042946495542321No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA1280580.39900708437143734Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG1252730.3903294950761613No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC1199500.37374392673908624No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1188770.37040064009139106No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1180290.3677584154154866No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA1176070.36644353473950575Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA1172470.36532183558464065Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1131480.352550044374107No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG1098440.3422553387972338Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTG1065480.33198555986824646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT1041970.3246602412207801No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1037230.32318333733354104No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTC1035270.3225726344603367No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG1015390.3163783624606926No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC1013580.3158143970522743No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG991440.30891594724985383No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC931750.290317552095993No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC918380.2861516860680634No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC898460.2799449507444764No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC829260.2583834003231802No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCAGATCGGAAGAGCACACGTCTGA817010.25456650736565306Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTCC814540.2537968971121762Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT806530.2513011164926013No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT793490.24723807288720098No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC777240.24217484753537924Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA765720.2385854102398109Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT738560.2301228132825507No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG715010.22278503131114136Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG705950.21996208843806414No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC681820.21244358826948215Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC670070.2087824868612418No Hit
GGGGCGGGCGGGCGTCCCGCGCGTGTAGATCGGAAGAGCACACGTCTGAA665090.20723080303034505Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTAGATCGGAAGAGCACACGTCTG663320.20667930094586967Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTG653780.2037067981854771Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC650730.2027564697348275No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG610290.19015604922850934No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA583110.1816872206092777No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC581570.18120738263747427No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTAGATCGGAAGAGCACACGTCTGA566340.17646197204619768Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC562130.17515020720120264No Hit
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG561550.17496948900402992TruSeq Adapter, Index 5 (100% over 49bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACG559190.17423415289139613No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG552300.17208734534222372Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACACG543900.16947004731420512No Hit
GCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTC528790.16476202669475734No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC512150.1595772839344919No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG511820.1594744615119626No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGAGATCGGAAGAGCACACGTCTG507520.15813465418809594Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC505490.1575021404979914Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT467080.14553423368177776No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGAGATCGGAAGAGCACAC464390.14469607514661464No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG463480.14441253452691263Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTC461660.14384545328750858No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGTCTGAAC460560.1435027118790776Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC444270.13842702320331293TruSeq Adapter, Index 5 (100% over 50bp)
GGGGGGGGGATGCGTGCATTTAGATCGGAAGAGCACACGTCTGAACTCCA419990.13086178556994482Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCAGATCGGAAGAGCACACGTCTG418300.1303352101333554Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGTGGGGGTGGGGGCCGGGCCGCCAGATCGGAAGAGCACACGTCTG417990.13023861937279754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGAGATCGGAAGAGCACACG414360.12910757272497522No Hit
GGGGGGGGGATGCGTGCATTTATAGATCGGAAGAGCACACGTCTGAACTC412360.12848440652782794Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT406570.12668034038708653No Hit
AGCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACAC402790.12550255627447818No Hit
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT399620.12451483785199972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA397090.12372653261260838Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA394420.12289460573941677No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTC384100.11967906816213675No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT383980.11964167819030792No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT377280.1175540714298645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT375480.11699322185243194No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA370290.11537610557083473No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA365760.11396463413429611No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG347730.1083467908670133No Hit
GGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGAAC347180.10817542016279781Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCCCGGGGCTACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGAA342800.10681068619104524Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCAGATCGGAAGAGCACACGTCT330640.10302183571238974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)