FastQCFastQC Report
Wed 6 Sept 2023
SRR13784006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13784006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62165157
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG59171219.51838825083318TruSeq Adapter, Index 6 (100% over 49bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC35681555.73979890374925No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC22974983.69579698801372No Hit
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG15188622.4432689842639665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG10755581.7301621228110144Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACA9794901.575625394141609No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT9658051.5536114547253537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA8739011.405773012042743No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT8458981.3607268779197323Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA8002641.2873191971509057Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG7097831.1417698180992288No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA6694261.0768508153208718Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT5759550.9264916679933745Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT5660770.91060173788349Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC5525100.8887776154092235No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG5226880.8408054048669097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA5074360.8162707608057677Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC4926480.7924825155673619Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC4705060.7568644924358512No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG4695970.7554022585352757No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT4348720.6995429931915076No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG4289080.6899491945303058No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC4070600.6548041051356148No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC3970360.6386793167754727No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC3818570.6142621018394596No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT3763790.6054500916003478Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT3734680.6007674041585708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG3393840.5459392630505221No Hit
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC3364320.5411906222644945No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA2836570.45629579926903424No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA2792480.4492034018348896No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2678930.4309375427138389No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC2646830.4257738784444798No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAGATCGGAAGAGCACA2378660.38263556545027305No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2251140.362122466770252No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC2221130.35729500369475464No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTC2188890.3521088187712612No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA2162150.34780737383161436No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC2087200.3357507807790142No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG1992840.3205718598925118Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG1906590.306697528327645No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1808390.2909008980706025Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1776540.28577744925505455No Hit
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC1773360.285265908682576Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1734370.27899390650617995No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA1647470.26501501476140404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTAGATCGGAAGAGCACACGTCTG1583530.25472951029464946Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1582910.25462977596919767No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT1581530.2544077866641598No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC1525870.24545421802763243No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAAC1457920.23452365768174607Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG1432250.23039433488441122No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCA1373960.22101769967378992No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA1366670.21984501704065512Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC1351480.21740152606708607Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1309930.21071771764366332No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1306100.2101016168912756No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT1289960.207505307193224No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1187100.19095906087714054No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGAGATCGGAAGAGCACAC1175530.18909788967475782No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA1154480.18571174846385413No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT1145100.1842028646368576No Hit
TAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACA1122180.18051591183144602No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC1109550.1784842271049038No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC1089800.17530720625381838No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1089250.17521873225543372TruSeq Adapter, Index 6 (100% over 50bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT1076360.17314522345692782No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1036640.16675579215540307No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGAGATCGGAAGAGCAC1020860.16421739271083963No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACA1009680.16241895761640238No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA978880.15746441370686154No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA965210.1552654326924647No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC941730.15148839727051602No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC941620.15147070247083907No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC940490.15128892861961243No Hit
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG912470.14678158055645224TruSeq Adapter, Index 6 (100% over 49bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTC878180.14126562891170694No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCAGATCGGAAGAGCACACGT853090.13722960596721406No Hit
GCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTC852710.13716847847742103No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG852190.13708483033349372No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC851500.1369738356809748No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG846900.13623387133084858No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA844640.13587032362839524Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACAC842540.1355325138163811No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC841430.13535395720145935No Hit
GGGGGCGGGCGGGCGTCCCGCGCGTGTAGATCGGAAGAGCACACGTCTGA817360.13148201330851622Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC803350.1292283392769361Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGGATGCGTGCATTTATCAGATCAAGATCGGAAGAGCACACGTC795070.12789640344670888No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAGATCGGAAGAGCACACGT790790.12720791487746103No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG776840.12496389255479562No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC771570.12411615078845534Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGAGATCGGAAGAGCACACG767090.12339548985615849No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG766640.1233231020392983No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG766390.12328288658548711No Hit
AGCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACAC760320.12230645536695098No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTG757490.12185121642980809Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCA753110.12114664167903573No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG752730.12108551418924268Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCA747130.12018468802387164No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG736740.11851333376347783No Hit
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT734080.11808544133492657Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA727370.11700605855463374No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA719970.11581568112182197Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACACG714060.11486498779372503No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACAC693140.11149975861880314No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT690520.11107830066286167No Hit
TAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGAGATCGGAAGAGCAC665350.10702940877314925No Hit
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAGATCGGAAGAGCACAC662500.10657095259970148No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCAAGATCGGAAGAGCACACGTC653720.10515858586185184No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA649050.10440736118465846No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACG634650.1020909510451329No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)