FastQCFastQC Report
Wed 6 Sept 2023
SRR13784008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13784008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44422187
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC29671506.679432509705116No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG22262435.0115564999084805TruSeq Adapter, Index 7 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC17350903.9059085497073793No Hit
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACA9379072.111348097292013No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG8719561.9628839975843602No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC7827721.7621194562077729No Hit
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG7764941.7479868787189607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA7405721.6671218821351592No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT6720981.5129781881292788Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG5665751.2754324770187473Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC4720191.0625748795303571No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA4363790.9823447008586048No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3864820.8700201995908036No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3750270.8442335358229888No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT3655600.8229221132223858Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC3651680.8220396713020905No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT3492570.7862219840729588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC3392220.7636319211388669No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA3179640.7157774559816247Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC3106030.6992069075752618No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG3083020.694027063548222Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG3081450.6936736365546343No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT2976000.6699354986732192Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2912950.6557421407460196Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG2754840.6201495662516571No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC2745450.6180357576721741No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC2655280.5977373423780329No Hit
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC2538480.5714441749569871No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2474670.5570797313513628No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA2377880.5352910697530493No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2290320.5155801986966557No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2166230.48764595943914246No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA2165330.48744335797785016No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA2101030.47296860913218886Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC2026600.45621346828331527Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAGATCGGAAGAGCACA1926580.4336976925516972No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1868900.4207131900102082No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT1855650.41773044627451594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1851130.416712936713359No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1617720.3641693732908738No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT1553470.34970588008195097Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTAGATCGGAAGAGCACACGTCTG1549460.34880317801552635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCA1527230.34379892192160644No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG1505990.33901752743510805No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTC1480010.3331690985858035No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC1428460.32156453710844984No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1353290.3046428128358471No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1295450.291622292256795No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG1286020.2894994791679212No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT1275970.28723709618349047No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT1238350.2787683551014722No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1233950.27777785906848756No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAAC1155530.2601245184078848Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA1072700.2414784305869497Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA1069930.24085486831163894No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1063070.23931059495112206No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT1005720.2264003796121069No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC995280.22405020266111617No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC989120.22266350821493774No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACA980270.22067126051223007No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC973390.2191224848970178No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA960230.21616000130745475No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC946400.21304669218559635No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA946210.2130039207659902Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACAC945590.2128643508704333No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC926280.2085174239620395No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA919130.20690786790843954No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGAGATCGGAAGAGCACAC892380.2008861022533627No Hit
TAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACA867890.19537309137886436No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC862840.19423627206827976No Hit
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC847600.19080555399039673Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACA839610.1890069032395906No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTC829240.18667248418003374No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG817430.18401390278240914No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG792300.1783568197576585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA784650.1766347073366739Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGCGGGCGGGCGTCCCGCGCGTGTAGATCGGAAGAGCACACGTCTGA776730.1748518144773016Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC756830.170372071055394Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACA754910.1699398546046371No Hit
GCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACACG749270.16867021878053864No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA748000.16838432560738173No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG744580.167614440054471No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACA740510.1666982312239602No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGAGATCGGAAGAGCAC721480.16241433588130183No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAGC720360.1621622096183603No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC717410.16149812705079108No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG682430.15362368358856354No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC660290.14863968764077284No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACG648570.1460013663892775No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCA645500.145310270293536No Hit
AGCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACAC629890.14179626050378832No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAGATCGGAAGAGCACACGT622290.14008540371954223No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG602070.13553362422250845No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG596080.13418519894124076No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT595990.13416493879511154No Hit
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAGATCGGAAGAGCACAC591410.1331339224698685No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCAGATCGGAAGA584070.13148159499666237No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT577950.13010390505987468No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACAC577150.1299238148720593No Hit
CGGGGAGGTGGAGCACGAGCGTACGCGTTAGGAGATCGGAAGAGCACACG568350.12794282280609012No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACAC563090.12675872982120398No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC534370.1202934920786318No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAAGATCGGAAGAGCACACG522180.11754936784179491No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG512100.1152802314753211No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC511450.1151339081977211Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT511220.1150821322687242No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACG505860.11387552801036113No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAGATCGGAAGAGCACACGTC504070.11347257621512422No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGAGATCGGAAGAGCACACG499000.11233125464984424No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGT475200.10697357156233664No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT474000.10670343628061355No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC473390.10656611751240434Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGATGCGTGCATTTATCAGATCAAAGATCGGAAGAGCACACGTC461240.10383099778495822No Hit
NGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC456210.10269868072906901No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGC455420.10252084166860133No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACAC449200.10112064045833674No Hit
GCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCA448450.10095180590725981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)