FastQCFastQC Report
Wed 6 Sept 2023
SRR13784010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13784010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43648186
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG483174611.069752131279866TruSeq Adapter, Index 8 (100% over 49bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC19720214.5179907362015No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC12069302.7651320950657605No Hit
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG7890931.807848326159534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT7879991.8053419218842224Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG7876211.8044759065130451Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA7336741.6808808503519481No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC5600481.2830957052831473No Hit
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACA5577681.2778721205046184No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG5336221.2225525248632325No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT5125501.1742756044890388Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC4604791.054978550540451Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC4413891.0112424832500486No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3947720.9044407939427311No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA3428620.7855125983929779No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC3317420.7600361673678718No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC2985950.6840948670810741No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2943020.6742594067941334No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2825110.6472456839328902No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG2671960.6121583151244819Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC2638070.6043939603813089No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2546420.5833965241991959Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT2512590.5756459157317557Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC2480720.5683443522715926No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2453630.5621379087781564No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC2240470.5133019731908217No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2089590.4787346718143109No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT2045320.4685922113693339Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA2017320.4621772826939475No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT1945070.445624475665495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA1939600.4443712735278392No Hit
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC1864000.4270509661042959No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1831720.41965546975995754Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTC1826030.4183518646112807No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1756940.4025230281047647No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCA1746390.4001059746217174No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1716910.3933519711449177No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT1709540.39166347027571774Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG1682000.3853539297142841No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA1642730.37635699224705466Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1496640.342887101883226No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1435910.328973579795504No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACA1402310.32127566538504027No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT1393850.3193374405066914No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1364300.31256739970820324No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACAC1323260.30316494710685116No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC1320520.30253720051504546No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAGATCGGAAGAGCACA1282030.29371896463234465No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1248890.2861264383358337No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA1218910.2792578825612593No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1173300.2688084219582459No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1164250.2667350253685227No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACA1141510.2615251868657268No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT1076580.2466494254766968No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT1076370.2466013135116314No Hit
TAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACA1009710.23132920117230074No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC946640.2168795743309928No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG935100.21423570729835142No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG931100.21331928891615334No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG924840.2118850941480134No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACG911440.2088150925676499No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA910340.2085630775125454Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC905430.20743817394839728Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACAC868590.19899796064835315No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC856410.19620746667456007No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC840510.1925647036053228No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA792270.18151269791601418No Hit
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG754800.17292814872077386TruSeq Adapter, Index 8 (100% over 49bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC747550.1712671404030399Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACA735400.1684835195671133No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAGATCGGAAGAGCACACGT731680.16763125047166907No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG723840.16583507044256088No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAAGATCGGAAGAGCACACG720880.16515692083973432No Hit
GCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACACG711430.1629918824117914No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTC709920.16264593447251163No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA708190.16224958352221097No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAAC691420.1584074994548456Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGAGATCGGAAGAGCACAC672540.15408200469087077No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA672020.15396287030118502No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG669050.15328242965240296No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG663480.15200631705519216No Hit
AGCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACAC659990.15120674201672435No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC652050.14938765152806122Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCAGATCGGAAGA622290.14256949876450764No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCA621270.14233581207704712No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG617620.1414995803032914No Hit
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC616850.1413231697647183Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC613860.14063814702402522No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT610910.13996228846715417No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC603510.13826691446008774TruSeq Adapter, Index 8 (100% over 50bp)
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA600770.1376391678682821Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAAAGATCGGAAGAGCACACGT596970.13676857040519394No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAGC595240.13637221945489328No Hit
TAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGAGATCGGAAGAGCAC589360.1350250844330621No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCA588990.13494031573270882No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCAAGATCGGAAGAGCACACGTC575900.13194133657696566No Hit
CGGGGAGGTGGAGCACGAGCGTACGCGTTAGGAGATCGGAAGAGCACACG573850.13147167215608913No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC560740.128468110908435Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGGATGCGTGCATTTATCAGATCAAGATCGGAAGAGCACACGTC546930.12530417644389621No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACA537750.12320099625675167No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCA532810.12206921955473707No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACAC532300.1219523762110068No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC529310.12126735347031375No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC529070.12121236836738186No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAAGATCGGAAGAGCACACGT527650.12088703984170157No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGAGATCGGAAGAGCAC525610.12041966646678054No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTAGATCGGAAGAGCACACGTCTG519190.11894881496335265Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCA512100.11732446338090659No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC508320.11645844800972943No Hit
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT492040.11272862519418332Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGAGATCGGAAGAGCACACG489290.11209858755642216No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG472920.1083481453272766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGATCAAAGATCGGAAGAGCACACGTC469000.10745005531272249No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACA468940.10743630903698954No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGT468330.10729655523370432No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG462290.10591276347658525No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA456050.10448315080035628No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG454190.10405701625263418No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC452270.10361713542917912No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG452060.10356902346411372No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGC450390.10318641878954603No Hit
TAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTAGATCGGAAGAGCACACG449810.1030535381241273No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACAC443360.10157581348283293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)