FastQCFastQC Report
Wed 6 Sept 2023
SRR13784012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13784012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33882289
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG339145310.009515590874042TruSeq Adapter, Index 9 (100% over 49bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG12785513.773508336464517Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC11948223.526391029838628No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA11151643.291288850053785Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA6781912.0016091592867293Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT6200371.8299737659400757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT6097091.7994917639714365Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA5863411.7305235782623778Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG5449301.6083033823364175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT4865301.4359419459529432Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG4153281.2257967577102007No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA4027211.188588527770364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG3813221.1254316377503304Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA3646091.0761049821633952No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC3405611.005129848222474No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3159030.9323543636617939No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3121030.9211390647190336No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT2814730.830737852451468Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC2673120.788943155522934Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2633350.7772054597609979No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2632260.7768837577650081No Hit
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACA2575200.760043100984116No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG2566340.7574281654937777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC2341130.6909598108911709Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA2138990.6313003233046032Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT2099230.6195655789371255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2065970.6097492409677516No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT2049270.6048204122218543Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC1838010.5424692528890241No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT1837510.5423216831660931Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC1492980.440637289883219No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1460670.431101334387414Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1441890.4255586155941235No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1403520.41423411505639424No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA1389370.41005789189744524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG1371650.4048280209167686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG1343710.39658182479938114No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1326070.3913755649743735No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA1313270.38759778006733847Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTC1263790.37299428028608106No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC1231840.3635645749907865No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG1168580.34489405364554915No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT1117970.32995704629046757No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAAC1111690.32810357057045353Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC1034020.3051800898103431No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC1018800.30068806744432175No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1017600.3003339001092872No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT955170.2819083445041154No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC917590.2708170041286172No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG843270.24888224051214486No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT839940.24789942615742402No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC835770.24666869466817898No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC807610.23835756787270188No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC800910.23638013358542573Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG798890.23578395190478424No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA774070.22845859085848658No Hit
GCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACACG763870.225448168510693No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC630250.18601163575459734No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCAGATCGGAAGAGCACACGTCTGA618540.18255555284355196Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTC610280.1801177010207309No Hit
GGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTG592860.1749763718738129Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA589810.17407619656393344No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC588370.173651195761892Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTCC583800.17230240849430215Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA578200.17064962759747432No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC561940.16585066020775632No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA558010.1646907621855182No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA544000.16055585854899002No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACG542300.1600541214910244No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG536990.1584869310334966No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT533630.15749526249539988No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT532730.15722963699412398No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC523710.1545674791924477No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG522140.1541041102624442No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGAGATCGGAAGAGCACACGTCTG520220.15353744252638893Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG519430.15330428236415786TruSeq Adapter, Index 9 (100% over 49bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG508570.1500990679820953Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA505470.14918413569992275No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC497580.14685548547207067TruSeq Adapter, Index 9 (100% over 50bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTC490900.14488395397371176No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG482230.14232509497808724No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCAGATCGGAAGAGCACACGT467680.13803081604079345No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC463500.13679713315708983No Hit
AGCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACAC454160.1340405307327377No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCAGATCGGAAGAGCACACGTCTG439960.12984955060149567Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA435530.1285420828563265Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG426910.12599798083299507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACA425250.12550804935286397No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTAGATCGGAAGAGCACACGTCTGA424380.12525127803496394Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCC423210.12490596488330527Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACA421400.12437176248629483No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT413460.12202835528614965No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC403050.11895595365472504No Hit
CCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACACGT399730.11797609069446283No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAGATCGGAAGAGCACACGTC396010.11687817195585576No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA394810.11652400462082123Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGAGATCGGAAGAGCACAC394000.1162849416696729No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG393300.11607834405756942No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGAGATCGGAAGAGCACAC380580.11232417030620334No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCAGATCGGAAGAGCACACGTCTG369590.10908058779617871Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA363170.10718579255374393Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACAC357840.10561269930729887No Hit
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT352150.10393335586034345Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT351590.10376807777066066No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT346720.10233074866931216Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)