FastQCFastQC Report
Wed 6 Sept 2023
SRR13784015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13784015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52636685
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG590893011.225877921453451TruSeq Adapter, Index 10 (100% over 49bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT18516833.5178564151598835Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC17998713.41942316466168No Hit
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG15153352.878857207667998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA11955712.2713645435688057Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG8860491.6833297917602523Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT8834061.6783085788932186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA7815231.4847496570120249Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC7283671.3837630542272943No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA6535631.2416492413988458Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG6305411.1979116845979187No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG5756541.0936365008548696No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG5364981.0192473177214711Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA5072000.9635865176539897Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT4231330.8038747120948061No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT4206340.7991270726870432Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT4137900.7861247341089204Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG3987080.7574717138816778Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG3951040.750624778137149No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC3905660.7420034145387385Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG3741090.7107381477385971No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC3646220.6927145962934406No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT3434300.6524537022040807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT2868400.5449431323420159Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG2852180.54186163129384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA2608390.49554602460242325No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA2542180.4829673449230323Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA2293890.43579682117139407Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC2142310.40699941495175845No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA2060650.3914855200322741Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT1985530.3772141045736448No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC1919840.3647342153101017Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT1906630.36222455878442955Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTC1889690.3590062710066183No Hit
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC1852910.3520187489010754Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTC1833020.3482400154948968No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG1817290.3452516054155006No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1653530.3141402236861991No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC1634770.31057616945292055No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1551480.29475260457606706No Hit
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACA1514390.28770618818415333No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC1439000.27338347770191074No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1413080.26845915543503546No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1412390.2683280681524682No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1391990.26445244414613117No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1365560.25943123127909745No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCAGATCGGAAGAGCACACGTCTGA1356680.2577441949469272Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTC1320610.25089155975533034No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC1264990.2403247848909938No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC1257330.23886952607292802No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT1242390.236031201432993No Hit
GCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACACG1233230.23429097026152007No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC1184930.22511486048181037Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCACACGTC1179250.224035765170242No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT1144500.21743390564964338No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1104450.20982514381367293Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA1102280.20941288380907727No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGAGATCGGAAGAGCACACGTCTG1100940.20915830850670022Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA1053050.2000600911702551No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1044080.19835595649688045No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACG1025660.19485649599704086No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT1021770.19411746769387167No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAAC998200.1896396021140009Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG969540.18419473034823525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT960740.18252289254158008Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG931290.17692793533635334TruSeq Adapter, Index 10 (100% over 49bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA918800.1745550655403166No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC918480.1744942714382564No Hit
GGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTG904950.17192382081052407Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG898490.17069653987518402Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC843930.16033114547392185No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC819870.15576018892527144No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG768900.146076828356497No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC766890.14569496540293145TruSeq Adapter, Index 10 (100% over 50bp)
CGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCC760270.14443728741656128Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCT752420.1429459321003973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG702320.133427855496599Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACAC700540.1330896883038892No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT697100.13243615170674217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT695960.13221957271815274No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT691010.1312791639519092No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC690170.1311195794340012No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT687230.1305610336213232Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCAGATCGGAAGAGCACACGT677690.12874860945365385No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG674540.12815016751149885Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT663950.12613826269644451No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC650510.12358491040991657No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC648150.12313655390722268No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCAGATCGGAAGAGCACACGTCTG644680.12247731786300751Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG641810.1219320707601552No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCAGATCGGAAGAGCACACGTCTGA621280.11803174914985623Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGAGATCGGAAGAGCACAC620440.11787216463194823No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT615480.11692985605001531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA613380.11653089475524532Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT605000.11493884920754413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA599650.11392244781372535Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG578130.10983404445017766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTG572140.10869605485223852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA571470.10856876720104999No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA570760.10843388028710393Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGAGATCGGAAGAGCACACG567730.10785823613322153No Hit
CCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC560800.10654166386048058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCTGA553070.10507310633258915Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT544990.10353805525556938Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA542520.10306880078029229Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA527880.10028747061103867Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)