FastQCFastQC Report
Wed 6 Sept 2023
SRR13784019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13784019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44881250
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG850653018.953415958780116TruSeq Adapter, Index 12 (100% over 49bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG7890741.7581373067817854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC7063191.5737507310959475No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT6400021.4259896950285476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT5514641.2287180058487677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA4778321.0646584041219886Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG3778280.8418392981478902Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA3706760.8259039131040246Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG3370320.7509416515805598No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT3154710.7029015457457178Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG2984590.6649970756162095Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT2869490.6393516223367219Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC2755570.6139690850856426No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC2582080.5753137446038157Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC2519190.5613012115304276No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2437870.5431822865896114No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2348970.5233744603815624Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2319370.5167792786519982No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT2207800.4919203453558001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA2027820.4518189667177273Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT1971810.4393393677760758Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1876830.4181768555911433No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT1849700.4121320150396881Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACA1798770.40078429188135356No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1737120.3870480434479877No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1691610.3769079515387829No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT1632970.3638423617880518Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC1598300.35611753237710625No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA1545140.3442729424871188Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1523660.33948697952931345No Hit
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA1453690.32389695028547555Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC1361460.30334716613285057Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC1331390.29664726361231025Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAA1316750.2933853223784988Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1307520.29132878429188136TruSeq Adapter, Index 12 (100% over 49bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1305710.29092549784152627No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1260130.28076980921877176TruSeq Adapter, Index 12 (100% over 50bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1256070.2798651998328924No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG1255350.2797047764935246No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTC1209250.2694332265701156No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT1205620.2686244255674697No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT1181460.26324133129090654Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA1176850.26221417629856564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG1175920.26200696281854896No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG1163210.2591750452583206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1122620.2501311795014622No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1087610.2423305946247041No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTGA1071080.2386475421250522Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA1062320.23669572482941095Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA1047580.23341150257624285Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTC1042720.23232864503551037No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC1040670.23187188413869936No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC972970.21678763403425705No Hit
CGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCC951910.21209525135774962Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG928510.2068814928282969Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT904810.2016008912407743No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA864430.19260381562456483No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC855180.19054282133407602No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTG837140.18652332544213898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTG836960.18648321960729705Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAGC833340.18567664670658685No Hit
CGCGCGCTACACTGACTGGCTCAGCGTAGATCGGAAGAGCACACGTCTGA799590.17815680267372233Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAAC795570.17726110569558556Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC780810.17397242723854617No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT778370.17342877036624424No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA774870.172648934688762No Hit
GGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTG766580.17080183818409692Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT737180.16425121849324606Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC719100.16022281019356635No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCAGATCGGAAGAGCACACGTCTGA700410.15605848767581118Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT650100.1448489068374878Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC643160.1433026040941373No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC615060.13704163765492272No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC610670.13606350090516642TruSeq Adapter, Index 12 (100% over 50bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC588070.13102799053056677No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT587800.13096783177830384Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTG572650.12759225734577356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGCGCTACACTGACTGGCTCAGATCGGAAGAGCACACGTCTGAACTC572160.12748308035092604Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC570960.12721570811864644Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT555950.12387132711321543No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGAGATCGGAAGAGCACACGTCTG551220.12281743489764656Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT541010.12054254282133409No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT537960.11986297173095668Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGTGGGGGTGGGGGCCGGGCCGCCAGATCGGAAGAGCACACGTCTG532240.11858849742375714Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC516470.11507478067121571No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA492490.1097317922294945Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG479840.10691324328088009Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA479020.10673053892215568No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTC477900.10648099150536137No Hit
GCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACACG474870.10580587661885531No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC455650.10152346469850997Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT450450.10036485169196491No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)