FastQCFastQC Report
Mon 3 Jul 2023
SRR1378560.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1378560.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157247848
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA21876001.3911796109286023Illumina Single End Adapter 1 (95% over 22bp)
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAAT21363801.3586068281201533No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATT19868931.2635422521012816No Hit
CACAGGATTATACATGGGCACTACGCTTTGCCTGTAGGCACCATCAATTC6754210.4295263869048306No Hit
TAAACAATACTATTTCGTTCTTTTTTGTTTCCTTCTGTAGGCACCATCAA5752380.3658161350481566No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATTC3932840.2501045356118324No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC3746850.23827671078843635Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG3441580.21886340854725084Illumina Single End Adapter 1 (95% over 22bp)
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGTA3314490.2107812629652013No Hit
CAAAGCGTAGTGCCCATGTATAATCCTGCTGTAGGCACCATCAATTCGTA3289330.2091812410685582No Hit
AACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAA3128690.19896552097806772No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTTCTGTAGGCACCATCAA3121760.1985248154238651No Hit
TGGATAAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCA2999210.19073138603461207No Hit
AACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAAT2744100.1745079525667022No Hit
ACTGCTAATGTAAAAGAGAAATTATATATGTGCCTCTGTAGGCACCATCA2531210.16096945250404954No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT2173290.13820793274067572Illumina Single End Adapter 1 (95% over 22bp)
ACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTATG2150600.13676498771544396No Hit
TACTGCTAATGTAAAAGAGAAATTATATATGTGCCTCTGTAGGCACCATC2096660.13333473409442145No Hit
TTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATTCGT2076160.1320310596555827No Hit
AAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATTCG2069100.13158208689762163No Hit
ATATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATTCGTA2016660.12824722408919706No Hit
TAAACAATACTATTTCGTTCTTTTTTGTTTCCTCTGTAGGCACCATCAAT1915200.12179498952507128No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATC1884140.11981976376554292No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA1864040.1185415268767303Illumina Single End Adapter 1 (95% over 22bp)
TTAACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCA1829910.11637106792075146No Hit
AAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAATTC1759800.11191250133992295No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGT1678190.10672260519584344No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGT1616850.10282175689933766No Hit
ATTAAAATATCCTTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAAT1584630.10077276224473355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)