FastQCFastQC Report
Sun 2 Jul 2023
SRR1378561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1378561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128881071
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATT9131890.7085516848319797No Hit
ATATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATTCGTA8909140.6912683089047267No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8323140.6458000337380809Illumina Single End Adapter 1 (95% over 22bp)
TTAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGT6864600.5326305831210854No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATTC6487320.5033570833687439No Hit
ACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTATG5956650.4621819134324233No Hit
ATATTCTTATTTGAAATTGAACATAGGCTGTAGGCACCATCAATTCGTAT4138640.321120857228134No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAATTC3976540.30854337018971545No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGT3896840.3023593744033986No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATTCG3888980.30174950982522486No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAAT3778890.29320752618512924No Hit
CACAGGATTATACATGGGCACTACGCTTTGCCTGTAGGCACCATCAATTC3657650.2838004038622553No Hit
ATATTCTTATTTGAAATTGAACATAGGTACTGTAGGCACCATCAATTCGT3610510.2801427682114777No Hit
AACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGGCACCATCAATTC3229950.25061477026366424No Hit
TCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATTCGTA3141270.24373400807632956No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATTCG3101070.24061485336353233No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGTA2831260.2196800490585619No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATTC2634190.20438920778366282No Hit
CAAAGCGTAGTGCCCATGTATAATCCTGCTGTAGGCACCATCAATTCGTA2403630.1864998468238986No Hit
TTCTCTCTTCTTTTAAGAATGTACTTGCTTGCTGTAGGCACCATCAATTC2322720.18022196603254484No Hit
AACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTAT2307330.17902784187757098No Hit
TTTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATTCG2247980.17442282117596616No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATTCG2083320.16164670139961826No Hit
ATTGGCCTAACATGGCTATGACGGGTAACGGGGCTGTAGGCACCATCAAT2023920.15703780115235075No Hit
TTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATTCGT1845940.14322817041146407No Hit
ACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGGCACCATCAATTCG1830080.141997578527261No Hit
TAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTA1764510.13690994234521842No Hit
TATAGTATTGTGACAGTAATCCAACTTGGCTGTAGGCACCATCAATTCGT1728520.13411744537721915No Hit
TTTTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATTC1637640.12706598318072637No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGT1610550.12496404534068468No Hit
TTTACTTACTACTGTGCATATAGAGGTGTCTGCTGTAGGCACCATCAATT1587150.12314841797054898No Hit
ACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATTCGTAT1561220.12113648558988155No Hit
TCTTCTTTCTTTTTTTCTCCCCCTCTTTTCGCCCTGTAGGCACCATCAAT1493560.1158866843991388No Hit
TTCTCCCCCTCTTTTCGCCTTCACTTTATTGCTGTAGGCACCATCAATTC1412480.10959561315253193No Hit
TCTTTATTGTAGATTTGTGTGGTGTTTACTGTAGGCACCATCAATTCGTA1370050.10630343070317906No Hit
TCTATGGAGACATAGCCAGGTGGGGAGTCTGTAGGCACCATCAATTCGTA1334760.10356524737445735No Hit
TCTTTATTGTAGATTTGTGTGGTGTTCTGTAGGCACCATCAATTCGTATG1326440.10291969097618688No Hit
ACGCTTGGATAAGGTGCCTAAATATTCGCTCCTGTAGGCACCATCAATTC1298530.10075412858727718No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)