FastQCFastQC Report
Wed 28 Jun 2023
SRR1378562.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1378562.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187293966
Total Bases9.4 Gbp
Sequences flagged as poor quality0
Sequence length50-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGNCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCNNA34418511.8376731901763457No Hit
CTNTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAANNA10643100.5682564274387782Illumina Single End Adapter 1 (95% over 22bp)
TTNTATCTTTGGATAAGTATTTGTTAGGCCCTGTAGGCACCATCAATNNG8279940.44208258156058267No Hit
ACNAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCANNT7382230.3941520465213492No Hit
TTNTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAANNC6874720.36705507106406193No Hit
TANTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTNNT6811510.36368016255259394No Hit
GTNACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGNNT5796300.3094760671574438No Hit
TTNATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATNNG4828290.2577920743052662No Hit
TTNTCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATTNNT4370180.23333266379761533No Hit
TTNATCTTTGGATAAGTATTTGTTAGGCCCTGTAGGCACCATCAATTNNT3734100.19937107851087954No Hit
ACNAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTNNG3577420.1910056194762836No Hit
AGNATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAATTNNT3406450.18187718871840217No Hit
CANAGCGTAGTGCCCATGTATAATCCTGCTGTAGGCACCATCAATTCNNA3201230.17092008185677482No Hit
AANTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAATNNG3195010.17058798359793395No Hit
TCNTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATTCNNA2827650.15097389736517192No Hit
AANGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATNNG2436720.13010136162101454No Hit
ATNACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTNNT2304920.12306429562178207No Hit
AGNATTTGTTAGGCCTTATAAGAAAAAAGTCTGTAGGCACCATCAATNNG2270370.12121960191712744No Hit
TANGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAANNC2249950.12012933721527366No Hit
TTNACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTNNT2144520.11450021833591799No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)