FastQCFastQC Report
Wed 28 Jun 2023
SRR1378563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1378563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62888377
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGTTATTATTAGATAGAAACTTCAATACTGTAGGCACCATCAATATC37182145.912402541410792No Hit
ATTACCATTATTTGTATGATCAATTTTCACTGTAGGCACCATCAATATCT18434132.9312459439047056No Hit
TTAATAATTAGACCAACAGATACAGCATTCTGTAGGCACCATCAATATCT15539242.4709239991994068No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCT14340652.280333931976015No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCT12255311.9487400668648198No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATAT7319091.1638223705471045No Hit
TTAGATTTAGCAATTTTTGCATTACATTTACTGTAGGCACCATCAATATC5583150.887787261547551No Hit
TAATGTTATTATTAGATAGAAACTTCAATACTGTAGGCACCATCAATATC5557260.8836704435861018No Hit
TATACTAGCATTTATTTTGATTCTTTGGTCTGTAGGCACCATCAATATCT5524770.878504147117678No Hit
ATATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATATCTC5478700.8711784691152071No Hit
TTTAATAATTAGACCAACAGATACAGCATTCTGTAGGCACCATCAATATC5434880.8642105678764774No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATC5100900.8111037751856753No Hit
AATGTTATTATTAGATAGAAACTTCAATACTGTAGGCACCATCAATATCT4070350.6472340667974306No Hit
ATATTCTTATTTGAAATTGAACATAGGTACTGTAGGCACCATCAATATCT3555320.5653381705175823No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACTGTAGGCACCATCAATATC3416790.5433102527037706No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATA3065870.48750979851173454No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATATC2941500.4677334891310679No Hit
TTTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATATC2571540.4089054484583058No Hit
AATTGTTATTATTAGATAGAAACTTCAATACTGTAGGCACCATCAATATC2516610.4001709250661692No Hit
TAGATTTAGCAATTTTTGCATTACATTTACTGTAGGCACCATCAATATCT2287580.36375243075520935No Hit
TCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATATCTC2025740.322116756169427No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATC1951700.31034351546391475No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATAT1784500.28375672662056456No Hit
TAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCTC1507340.23968498980344177No Hit
TATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATATC1380230.21947298783048574No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCCTGTAGGCACCATCAATATC1374610.21857934098060758No Hit
TTTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATAT1347800.2143162320757618No Hit
TTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATATC1343030.21355774533663033No Hit
TCTTTATTGTAGATTTGTGTGGTGTTTAACTGTAGGCACCATCAATATCT1314370.2090004644260417No Hit
TTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATATC1300500.20679496944244563No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAATAT1259880.20033590626770348No Hit
TTTCTTTATTGTAGATTTGTGTGGTGTTTACTGTAGGCACCATCAATATC1148620.1826442428304359No Hit
TTTTTGGTACATAAATAATCCTATCACCTTTCTGTAGGCACCATCAATAT1104040.17555549255150915No Hit
TATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATAT989420.15732954914705463No Hit
AAAAATATTCCTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAATAT983400.15637229753917803No Hit
TTTTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATA976850.15533076962695347No Hit
TTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATATCT951920.15136660308470037No Hit
TCTTTATTGTAGATTTGTGTGGTGTTTACTGTAGGCACCATCAATATCTC947180.1506128867024188No Hit
TTTAATATACGCATAATTAATGTAATATGTCTGTAGGCACCATCAATATC920500.1463704493439225No Hit
TTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATATC917200.14584571009043532No Hit
AAAAATATCCTTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAATAT915920.1456421748648403No Hit
TATAGTATTGTGACAGTAATCCAACTTGGCTGTAGGCACCATCAATATCT906660.14416972471717626No Hit
TTTTTTGGTACATAAATAATCCTATCACCTTTCTGTAGGCACCATCAATA852270.13552106774833766No Hit
AACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCTCG841050.13373695428648127No Hit
TTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATAT816090.12976801738737828No Hit
TTTCTTTATTGTAGATTTGTGTGGTGTTTAACTGTAGGCACCATCAATAT815870.12973303477047915No Hit
ACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATCTCG812330.12917013266219288No Hit
TTTTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATAT811350.1290143010050967No Hit
AACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATCTC807980.1284784309189598No Hit
TAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATCT798230.1269280649427477No Hit
TTTAATATACGCATAATTAATGTAATATGCTGTAGGCACCATCAATATCT766470.12187784715767112No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATA765460.12171724514372505No Hit
TAATATACGCATAATTAATGTAATATGTTCTGTAGGCACCATCAATATCT761530.12109232839639032No Hit
TTAATATACGCATAATTAATGTAATATGTCTGTAGGCACCATCAATATCT748540.11902676388039081No Hit
AGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAATATCT741160.11785325609531949No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTCTGTAGGCACCATCAATATCTC739130.11753046194847738No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACTTCTGTAGGCACCATCAATAT716670.11395905478686467No Hit
TTTTTTTGGTACATAAATAATCCTATCACCCTGTAGGCACCATCAATATC698840.11112387269908396No Hit
TAATATACGCATAATTAATGTAATATGTCTGTAGGCACCATCAATATCTC674530.10725829353172844No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAAT666710.10601482051285883No Hit
ATATTCTTATTTGAAATTGAACATAGGCTGTAGGCACCATCAATATCTCG656870.10445014346609709No Hit
AAATATTCCTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAATATCT638760.10157043804771745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)