FastQCFastQC Report
Thu 29 Jun 2023
SRR1378564.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1378564.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155108981
Total Bases10.8 Gbp
Sequences flagged as poor quality0
Sequence length70
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAGTATTTGTTAGGCCTTATAAGAAAAAAGTTCTGTAGGCACCATCAA63788644.11250461377217No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACTCTGTAGGCACCATCAATA34047902.195095331069192No Hit
AAGTATTTGTTAGGCCTTATAAGAAAAAAGTTCTGTAGGCACCATCAATA30450991.9631996679805408No Hit
ATAAGTATTTGTTAGGCCTTATAAGAAAAAAGTCTGTAGGCACCATCAAT21401561.379775681718907No Hit
ATAAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAATA17629641.1365969840263472No Hit
TATTCTTATTTGAAATTGAACATAGGTAACTCTGTAGGCACCATCAATAT14753930.9511976614687451No Hit
TTTTCTTCTTTCTTTTTTTCTCCCCCTCTTTTCGCCTGTAGGCACCATCA13753420.8866939819558224No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATA12749860.821993666504714No Hit
TTTTTCTTCTTTCTTTTTTTCTCCCCCTCTTTTCGCCTGTAGGCACCATC12676330.8172531286244477No Hit
TAAACAATACTATTTCGTTCTTTTTTGTTTCCTTCTGTAGGCACCATCAA11589160.7471624096350682No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAAT10395630.6702145764209488No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATAT9937420.6406734114254803No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAATAT9846360.6348027004316404No Hit
AAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAATA9504960.6127923695146962No Hit
TTTTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAAT9074160.585018349130925No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATA5418910.34936145960497283No Hit
TTTCTTCTTTCTTTTTTTCTCCCCCTCTTTTCGCCTGTAGGCACCATCAA5402440.3482996255387688No Hit
TTAAACAATACTATTTCGTTCTTTTTTGTTTCCTTCTGTAGGCACCATCA5025280.3239838188350938No Hit
TTAAGTATTTGTTAGGCCTTATAAGAAAAAAGTTCTGTAGGCACCATCAA4480220.2888433649112813No Hit
AAGTATTTGTTAGGCCTTATAAGAAAAAAGTCTGTAGGCACCATCAATAT4410970.2843787620524694No Hit
AACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAA4337890.2796672360319355No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4239630.273332335282378Illumina PCR Primer Index 6 (96% over 29bp)
CTCTCTTCTTTTAAGAATGTACTTGCTTGATTCTGTAGGCACCATCAATA4020510.2592054937167049No Hit
AATTCCCTTCGGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCA4009650.25850534083516413No Hit
ACGCGGAAGACCCGGGTTCAATTCCCGGTCTCGGAGCCTGTAGGCACCAT3967920.25581497437598405No Hit
TGGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCATCAATATCT3936480.25378801244268373No Hit
ACTGCTAATGTAAAAGAGAAATTATATATGTGCCTCTGTAGGCACCATCA3928630.2532819166673527No Hit
ATTATAAGGATAACTACGGAAAAGCTGTAGCTCTGTAGGCACCATCAATA3864430.24914289134553724No Hit
ATTTCTCTCTTCTTTTAAGAATGTACTTGCTTGCTGTAGGCACCATCAAT3795990.24473050983424358No Hit
TTTTTTCTTCTTTCTTTTTTTCTCCCCCTCTTTTCGCCTGTAGGCACCAT3761320.2424953072188644No Hit
TTTATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAA3738000.24099184817673452No Hit
ACGCGGAAGACCCGGGTTCAATTCCCGGTCTCGGAGCCCTGTAGGCACCA3568560.23006791592551304No Hit
AAAACAATACTATTTCGTTCTTTTTTGTTTCCTTCTGTAGGCACCATCAA3426680.2209207989058996No Hit
TTCTCTCTTCTTTTAAGAATGTACTTGCTTGATTCTGTAGGCACCATCAA3373480.2174909523775416No Hit
AGTTTTATCAAAATGAAAATCACAAAGATCTTTTCTGTAGGCACCATCAA3328880.21461555472406849No Hit
GTATAGTGGCAAGTATTTCCGCCTCTGTAGGCACCATCAATATCTCGTAT3153280.20329448234851083No Hit
ATACATTTATTCAGTAATCAAATTAGGATATTTTTCTGTAGGCACCATCA3134150.20206115595588886No Hit
AAAAGATTCTTTTTAAAATCCCCACTTTTGCTTCTGTAGGCACCATCAAT3093290.1994268790921913No Hit
TCTTCTTTCTTTTTTTCTCCCCCTCTTTTCGCCTGTAGGCACCATCAATA3090590.19925280793379718No Hit
GAAAAGCTTCTTAGAGGAACATTGTGTGTCTCTGTAGGCACCATCAATAT2965430.19118364268023913No Hit
ATTAAAATATCCTTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAAT2963970.1910895153131075No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATATC2950700.19023398780500014No Hit
TTTACCCTACTGATGAATTTTATTATATTTTTTCTGTAGGCACCATCAAT2923330.1884694220252791No Hit
AACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAAT2826830.18224798988267482No Hit
TAACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCA2808380.1810585036336484No Hit
TAAGTATTTGTTAGGCCTTATAAGAAAAAAGTTCTGTAGGCACCATCAAT2706270.17447539030638076No Hit
AAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAATATC2659170.17143881565439462No Hit
TATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAATA2623940.16916750939134853No Hit
TATATTCTTATTTGAAATTGAACATAGGTAACTCTGTAGGCACCATCAAT2574560.16598394131671848No Hit
TTTCTCTCTTCTTTTAAGAATGTACTTGCTTGATTCTGTAGGCACCATCA2516550.1622439902432213No Hit
AAAAGATTCTTTTTAAAATCCCCACTTTTGCTTTCTGTAGGCACCATCAA2485770.16025957903752847No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTTTTTCTGTAGGCACCATCAA2460150.15860783715676657No Hit
ATTAAAACAGTTATAGTCTACTTGACATTTTTCTGTAGGCACCATCAATA2455620.1583157844354609No Hit
AAAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAAT2304370.14856457602542048No Hit
ATTTCTCTCTTCTTTTAAGAATGTACTTGCTTGACTGTAGGCACCATCAA2278200.1468773752049857No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTTCTGTAGGCACCATCAA2223320.1433392177336269No Hit
ATTAAAATATTCCTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAAT2222590.1432921540500611No Hit
ATACATTTATTCAGTAATCAAATTGGGATATTTTTCTGTAGGCACCATCA2140310.13798749667499913No Hit
ATGAATTATCCGAAGTGGTAAGGACTATCCTTGCTGTAGGCACCATCAAT2134720.1376271049063239No Hit
TAAACAATACTATTTCGTTCTTTTTTGTTTCCTCTGTAGGCACCATCAAT2111450.1361268694041643No Hit
AAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATAT2078170.1339812811999584No Hit
CCTTCGGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCATCAAT2057070.1326209473325081No Hit
TATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATATC1941650.12517972766515692No Hit
ATTTGTTAGGCCTTATAAGAAAAAAGTTCTGTAGGCACCATCAATATCTC1935090.12475679922105865No Hit
AAACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCA1884070.121467499035404No Hit
TAACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAA1879810.12119285342993774No Hit
ATACATTTATTCAGTAATCAAATTAGGATATTCTGTAGGCACCATCAATA1835820.11835678296410186No Hit
TTATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAAT1819420.11729946185385616No Hit
ATACATTTATTCAGTAATCAAATTGGGATATTCTGTAGGCACCATCAATA1721020.11095553519238192No Hit
ATGATGAATTGTTTGGACAAGAAAAATTGAATTCTGTAGGCACCATCAAT1694180.10922513893634567No Hit
TATTCTTATTTGAAATTGAACATAGGTAACTATCTGTAGGCACCATCAAT1687550.10879769753628901No Hit
ATTATAAGGATAACTACGGAAAAGCTGTAGCTACTGTAGGCACCATCAAT1679230.10826130048523754No Hit
TTCTCTTCTTTTAAGAATGTACTTGCTTGATTCTGTAGGCACCATCAATA1667260.10748958501635697No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACTATCTGTAGGCACCATCAA1666880.107465086112583No Hit
TGATGAATTGTTTGGACAAGAAAAATTGAATTCTGTAGGCACCATCAATA1657080.10683327227841179No Hit
ATTATAAGGATAACTACGGAAAAGCTGTAGCCTGTAGGCACCATCAATAT1638800.10565474606528426No Hit
ACATATAGTATTGTGACAGTAATCCAACTTGGTCTGTAGGCACCATCAAT1587500.10234739405579615No Hit
AACTACGGAAAAGCTGTAGCTAATACTTGCTTTCTGTAGGCACCATCAAT1584480.10215269224159239No Hit
TTTTCTCTCTTCTTTTAAGAATGTACTTGCTTGATTCTGTAGGCACCATC1571750.10133197896516385No Hit
TATTCTTATTTGAAATTGAACATAGGTAACTATACACTGTAGGCACCATC1567520.10105926748367976No Hit

[FAIL]Adapter Content

Adapter graph