FastQCFastQC Report
Wed 6 Sept 2023
SRR13794628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13794628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77437343
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA38889055.022002110790398No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC37842294.88682701832887No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA18128072.3409984508378603No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA15909852.054544924145964No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA15366631.9843953065383453No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA13644131.7619574060024246No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10894841.4069232721479092Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA10703651.382233633713388No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT8787501.1347884185540817No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA8107681.0469987328981574No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT7553420.9754234465405147No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG6757680.8726642390093369No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA6732330.8693906246240912No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT4674290.6036222084737591No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT4533780.5854772160764865No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT4422570.5711159278799118No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG4190010.5410839057326644No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT3769450.4867741911031219No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3710560.47916933306970516No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA3671860.4741717442448922No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT3487340.45034344734684406No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT3128440.4039962993048457No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA2823750.3646496497174496No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC2470840.31907603028166914No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC2455720.31712348395011436No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG2253370.29099268036611226No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG2226540.2875279437208996No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA2203030.28449194079399137No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA2088920.2697561562772111No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT2044810.26405993811022155No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATC2008050.25931287440996004No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA1960440.253164677925481No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA1749410.22591296811410483No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC1747540.2256714825559033No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC1731630.223616918261258No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1721970.22236945810498687No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT1696690.21910488328609107No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT1537880.19859668997165877No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG1537490.1985463266734242No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA1499560.19364817307845905No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGACTGTAGGCACCATCAATA1470460.18989029621018894No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGACTGTAGGCACCATCAAT1328540.17156322111929898No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA1283840.16579081232164694No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA1282200.16557902819573755No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT1272990.16438967953742936No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1234470.1594153353118017No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1196560.15451976445007934No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG1175810.1518401787106771No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGATCG1172140.15136624716062377No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1143000.14760320482586806No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT1127580.14561191749567132No Hit
CCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCGG1077370.1391279656896286No Hit
TCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1075540.13889164559791262No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAA1067960.13791278969889242No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA1031240.13317089146511651No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1014590.13102076604048774No Hit
TTACCCGTCGCCGCGCTCTCTCTCTCCCCCTGTAGGCACCATCAATAGAT1010920.13054683449043442No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1001270.1293006657007847No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATCGG995670.12857750039280144No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGA965650.12470081779536264No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATC954540.1232661094789887No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC923750.1192899916517022No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA920690.1188948334655542No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA908520.11732324028731203No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT898290.11600217223362119No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA884270.11419167623042026No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAAT881530.11383784177615701No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGATCGG871710.11256971975394353No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAG871280.11251419098922337No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG866380.11188142134473802No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGATC864430.11162960485356528No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG862030.11131967686442962No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG843160.10888286804985031No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGAT841630.10868528895677632No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGCTGTAGGCACCATCAATAG837060.10809513441079711No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT831280.1073487245036287No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCGG825550.10660877142956726No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA823370.10632725350610235No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG821450.10607931111479381No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA803020.10369932243155604No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATCG794060.10254225793878285No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATCG787280.10166671136947454No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAG786360.10154790564030586No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT777720.1004321648794174No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)