FastQCFastQC Report
Wed 6 Sept 2023
SRR13794629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13794629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81638157
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT42359835.188729334004931No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA21294102.608351386472382No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA13361641.636690549003942No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG11075801.3566940272794252No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT10067051.2331304833351395No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7479920.9162284249018506Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA6446890.7896907814810176No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG6110690.7485090580866494No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAAT6060280.7423342494123183No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT5113900.6264105153672198No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG5049070.6184693757846592No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT4448530.5449081855191806No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG4021870.4926458592150727No Hit
TTTAAAGCATCTGGCCTACACCCAGAAGATTCTGTAGGCACCATCAATAG3932660.48171837098184367No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA3754100.4598462456716165No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGAT3562710.4364025513216816No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAATA3126150.3829275567796074No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT2987980.3660028728968No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA2924480.3582246473300469No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2226560.2727352113056643No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA2209210.2706099795956932No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT2079830.2547619981181104No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCA2067520.25325412478383114No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC2029600.2486092379571969No Hit
CTGGATTGAGGCTCCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1885660.23097777672761527No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC1880330.23032489574697285No Hit
CTGGATTGAGGCTCCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1877730.23000641721983017No Hit
TCCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1807160.22136217504273156No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA1783030.21840644932736542No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGC1774970.2174191658932232No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA1751040.21448793852609876No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCACTGTAGGCACCATCAATAGA1704670.2088079964862509No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC1660410.20338651202035343No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTCTGTAGGCACCATCAATAGA1619510.1983765998049172No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT1402140.17175057001838No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCA1322090.16194510613462282No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC1253390.15352992351358447No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAAT1173620.1437587573173657No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1166450.14288049153289925No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCAT1152040.14111538554208175No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC1136690.13923513731452805No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA1108500.13578209512985454No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1058560.12966485757389157No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG1055180.12925083548860614No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1019900.12492932685876287No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGAT1015180.12435116584025775No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA986410.12082707844568319No Hit
ACCTCTAGCATTACAAGTATTAGAGGCACTGTAGGCACCATCAATAGATC971520.11900317641908549No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA966600.11840051705233866No Hit
CCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG955810.11707883116469668No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTCTGTAGGCACCATCAATAG954180.11687916962652647No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATC951630.11656681568644428No Hit
TTGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA925650.11338448024984199No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAAT921100.11282714282734235No Hit
TGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCCTGTAGGC904370.11077785599692067No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT885870.10851175878455954No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA875640.10725866827199443No Hit
TCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCA846670.10371008252917811No Hit
TCGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGA836660.10248394019967894No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAG834930.10227202948738788No Hit
TGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCAAT822370.10073353321780648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)