FastQCFastQC Report
Wed 6 Sept 2023
SRR13794630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13794630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77038096
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA853265411.075888999125835Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA76351759.910908234284504No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA56926637.389412895147357No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA21209952.7531768178694342No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT18776372.4372837563379033No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA16549492.1482215759849517No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT8894621.1545742251989197No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC7865311.0209637060604406No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT7176380.9315365218787338No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC7172250.9310004234787941No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT6479820.8411189186191724No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG5861480.7608547334814713No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT5279570.6853193775713252No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATCG4414880.5730775070038076No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAATA4391140.5699959147484642No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATCG4143170.537807943747727No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG3809060.49443849183396227No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3797510.4929392335968428No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT3744620.4860737991240074No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA3657550.4747715987165622No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA3407830.4423564673768677No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG3226990.4188823669785401No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATAG3219310.4178854576052866No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA3152200.40917418312103665No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATCGG2949290.3828352663336851No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT2717050.35268914226540593No Hit
CTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCG2640330.34273043300550937No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT2453460.31847360298208827No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAGA2441740.3169522777406129No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGATC2370870.3077529330423742No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATA2366480.3071830851063609No Hit
CTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG2341170.3038976975754957No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA2275350.2953538727125343No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2006090.2604023339309943No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1892570.2456667672575916No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA1887260.24497749788624062No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA1866800.24232166900905755No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA1619490.2102193699075844No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC1607310.20863833394844025No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1602360.2079957947039605Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACCCGTCGCCGCGCTCTCTCTCTCCCCCTGTAGGCACCATCAATAGATC1565930.20326696547640533No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG1533280.19902880258099837No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1530660.198688711102102No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGAT1475850.19157404928595326No Hit
CCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCGG1415460.1837350705033001No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGATCG1368200.177600443292368No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAG1321760.17157225692597594No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT1249980.1622547888514794No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1232280.1599572242803093No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAG1198230.15553733311373635No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCGG1181950.15342409293189177No Hit
TTGTTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1176970.15277765951017272No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGAT1093200.1419038185990474No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1076470.13973216575861377No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCCTGTAGGCACCATCAATAGATCG1043250.13542001349566066No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCTGTAGGCACCATCAATAGATCG1005770.130554888064731No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG992540.12883755590221233No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCTGTAGGCACCATCAATAGATCGG988610.1283274186838678No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCAT904950.11746785642261978No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATA882150.11450828172077357No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG875800.11368401420512782No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGATC867860.11265335529580067No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG867840.11265075917764114No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA840460.10909667341726617Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATC835780.10848918176793985No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA826640.10730275576904184No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA819340.10635517264081917No Hit
TTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATAGATCGG812140.10542057010339405No Hit
TTTTGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA792200.10283224029835837No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT790600.10262455084559723No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC786320.10206898155946117Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC785020.10190023387909276Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)