FastQCFastQC Report
Wed 6 Sept 2023
SRR13794631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13794631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35246390
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT17967125.0975773689163635No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA7704612.185928828455907No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA5768741.6366896014031507No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG4645211.3179250414014032No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT3500740.9932194474384469No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAAT2596720.7367336059097116No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2163090.6137054035888498Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT1978780.561413523484249No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1864470.5289818333168305No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1791420.5082563065323853No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1786510.5068632560667915No Hit
TTTAAAGCATCTGGCCTACACCCAGAAGATTCTGTAGGCACCATCAATAG1639750.4652249492784935No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1525040.43267977231143384No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA1349420.3828533929290347No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGAT1328150.37681873235812235No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT1256780.3565698501321696No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG1087040.3084117267044937No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC1000920.2839780187417775No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCA987270.280105281704027No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA950190.2695850553773025No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA949600.26941766234783193No Hit
TCCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG820060.23266496228408073No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAATA762780.21641365257548362No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT757870.21502060210988982No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC738540.2095363525172365No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGC714040.20258528603922274No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG691510.1961931420494411No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC679110.19267505126056883No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC675640.19169055327368278No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTCTGTAGGCACCATCAATAGA655380.18594244687186404No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC654880.18580058837231275No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA652790.1852076198441883No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGAT610900.17332271475178027No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAAT598610.16983583283280926No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCACTGTAGGCACCATCAATAGA571740.1622123570669223No Hit
CTGGATTGAGGCTCCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC568650.16133567153969527No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA567000.16086753849117597No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC544700.15454064941118792No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA533510.1513658561912298No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT529960.15035866084441557No Hit
CTGGATTGAGGCTCCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA529100.1501146642251873No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCA462680.1312701811447924No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA436340.12379707538842985No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA411820.11684033457043402No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG387580.1099630345121869No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT385880.10948071561371249No Hit
TGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCCTGTAGGC381590.10826356968756234No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA357080.10130966603955753No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTCTGTAGGCACCATCAATAG356960.10127561999966521No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCAT354070.10045567787225869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)