FastQCFastQC Report
Wed 6 Sept 2023
SRR13794632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13794632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64472230
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA22280433.4558181095954024No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA14283952.2155197671927898No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA14227612.2067811211121438No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC14055462.180079702532393No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA12523081.9423990763154926No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA11972291.8569684963588198Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA11602331.7995856510624808No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT8762331.3590859196277219No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT8213151.2739050595892216No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT6104800.9468882959376463No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA5971290.9261801553940355No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG5575310.8647614639667341No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC5134180.7963397574428556No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT4406830.6835237434783937No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT4313470.6690430903351722No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA4288530.6651747581865867No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC4102530.6363251278883948No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA3824640.5932228495896605No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA3702330.5742518910855108No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT3460320.5367147995346213No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC3368420.5224606004786867No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT2588630.4015108520366056No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG2554090.39615350671133914No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA2509590.3892513102152663No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA2453020.3804769898605958No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA2395270.3715196449696249No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT2256710.3500282214528643No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT2175470.33742744744520237No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG2114660.3279954796041645No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT1982110.30743624037822176No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG1877830.2912618347465258No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1857980.28818298979265955No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATCG1835960.2847675658186478No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA1796600.2786626117942562No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA1744310.27055214314752263No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG1738180.26960134619199616No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1572150.24384917351237886No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT1543070.23933870443134975No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1527240.2368833837452187No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGACTGTAGGCACCATCAATA1526330.23674223770451247No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA1514130.23484995012581386No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGATCG1509750.23417058786395323No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA1498870.2324830395970482No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1498760.23246597798773208No Hit
CTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1468960.2278438329184519No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT1463930.22706365205608678No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC1384110.21468312791414226No Hit
CTGGATTGAGGCTCCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1373450.21302970286586953No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA1367040.21203547635935657No Hit
GTCGCGGCGTGGGAAATGTGGCGTACCTGTAGGCACCATCAATAGATCGG1323420.20526977273781286No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT1318380.2044880408200554No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT1316780.20423987195727525No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1228230.19050527645778656No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATCGG1156520.1793826582390589No Hit
TCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1149710.1783263895168509No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGATC1129930.17525840195073134No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATC1112230.172513033906226No Hit
CCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCGG1099250.17049976400692204No Hit
GTCGCGGCGTGGGAAATGTGGCGTACTGTAGGCACCATCAATAGATCGGA1087430.16866641653313372No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATAGATCG1079040.16736508105893033No Hit
CGCGGCGTGGGAAATGTGGCGTACCTGTAGGCACCATCAATAGATCGGAA1072700.166381711940164No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGCTGTAGGCACCATCAATAG1055750.16375267305008684No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGAT1019790.15817507785910306No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT991540.1537933463756411No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT987620.15318533266182974No Hit
CGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGATCGGA972350.15081687107767172No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG968160.15016697886826624No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAGA966360.14988778889763857No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATC964640.1496210073701499No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATAGATCG927960.14393173619091507No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA906800.14064970298064763No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA883150.1369814569776786No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGATCGGA854750.132576459663331No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGA852050.1321576747073895No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT845750.13118050981019272No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGAT771000.11958637075218277No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATCG764640.1185998995226317No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATAG763280.11838895598926856No Hit
CGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGAA735000.11400257133962947No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG725040.11245772016882306No Hit
TGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG719550.11160619075840869No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA702430.10895078392666115No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGCTGTAGGCACCATCAATAGAT691330.10722911244112389No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA683350.1059913702380079No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG682710.10589210269289584No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA668970.10376095258377133No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAATA666560.10338714823420875No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGATC663730.10294819955816636No Hit
GGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC657080.10191674772223637No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG653480.10135836778098105No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATCG650620.10091476593876154No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATC649130.10068365868529754No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG646980.10035018177593671No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)