FastQCFastQC Report
Wed 6 Sept 2023
SRR13794633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13794633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88966946
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT43209734.8568296364809465No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA15430451.734402572389076No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA13337441.4991455365906343No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG11284831.2684295131362608No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG9014071.0131931470368782No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT8633440.9704098418754309No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA7520440.8453071998222801No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAAT7157550.8045178936455794No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA5206070.5851690132198086No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT5096980.5729071558778694No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG4922840.5533335942542076No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4900110.5507787128041913Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG4295830.4828568578716864No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA4182040.4700667144402147No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT3625090.40746481283059893No Hit
CTGGATTGAGGCTCCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC3500980.3935146880280683No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT3480530.39121608153212317No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG3382480.3801951344941075No Hit
TTTAAAGCATCTGGCCTACACCCAGAAGATTCTGTAGGCACCATCAATAG3374030.3792453435459052No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA3146910.35371676127895857No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGAT2977920.33472206632786966No Hit
CTGGATTGAGGCTCCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA2962480.3329865903231072No Hit
TCAACCCGGCGGCGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2435790.2737859519197164No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC2370590.2664573874436468No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC2338570.2628582979570862No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2147670.24140089061841014No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCA2112230.23741738870074286No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT2055430.2310329951080933No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAATA2036820.22894120699613538No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA1982010.22278049198181984No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC1946460.21878462592163161No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA1870730.21027247580241767No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1843750.20723988884590916No Hit
TCCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1829780.20566964274574517No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA1664630.18710656876993395No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC1655330.18606123672043323No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC1650690.18553969470863932No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1590280.17874953243871045No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTCTGTAGGCACCATCAATAGA1529820.1719537501039993No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGC1401020.15747646322489253No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1376530.15472375549454065No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGAT1357900.15262971935666983No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCACTGTAGGCACCATCAATAGA1289610.1449538348770565No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1265670.14226294785930946No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCAT1227250.13794449008061938No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1179710.13260093248564472No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCA1004760.11293632581251017No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG1001230.1125395492388825No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAAT936660.10528179757906943No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA919100.10330803082753903No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAG913860.10271904803835798No Hit
TGACGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT905030.10172654459781053No Hit
AGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATAGATCG896200.10073404115726306No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)