FastQCFastQC Report
Wed 6 Sept 2023
SRR13794642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13794642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57843907
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA39482256.825654083151749Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA10534711.8212307132019971No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC7637341.3203361245982226No Hit
TTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA5495350.9500309168258638No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA4991200.8628739410704053No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC4980960.8611036595436059No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4867000.841402362395749No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA4154300.7181914596467351No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG3820780.6605328371059029No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT3739670.6465106169263428No Hit
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3677490.6357609972645866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG3593200.6211890216890087No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC3541690.6122840215478529No Hit
TGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG3483060.6021481225325945No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3377070.583824671455889No Hit
TAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGC3340910.5775733648143788No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3322230.5743439840604129No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3202500.5536451747631778No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA2919780.5047688082342018No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2292520.3963286919744201No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA2288430.39562161663803236No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2224190.3845158661222521No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA2111850.36509463304406464No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT2045330.35359471828208283No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG1936260.3347387997148948No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA1856160.3208911873812397No Hit
TAACCTCGGGCCGATCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1762080.30462672585377054No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT1700930.29405517161902633No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1593970.27556402785862993No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1557560.2692695014532819No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1427520.24678830909537283No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1394330.24105045324825652No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1381600.23884970287363197No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1352980.23390190430947205No Hit
TGTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAG1314560.22725989100286742No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1283220.2218418614081514No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAG1257490.21739368331395734No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1237920.21401044020072849Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1226500.21203616138861436No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG1208070.20885000039848625No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT1179530.20391603215875442No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1172120.2026349983585998No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA1133320.19592729101096162No Hit
TAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCA1116890.19308688813153646No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG1104560.1909552893790525No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA1101670.19045566890908666No Hit
ACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1099600.19009780926450906No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGATCGG1071630.18526238208632761No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA1059000.18307891961723818No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1035150.1789557541471049Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGCCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA990880.171302398366694No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGATCGGA985460.1703653938866889No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT980680.1695390320021087No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG972120.16805918728829985No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT970870.1678430884691105No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG970810.1678327157257894No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA970680.16781024144859372No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG938380.16222624796074028No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT909840.15729227972100845No Hit
AACCCGGCGGCGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG909790.1572836357682409No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT905350.15651605276248023No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA895380.1547924485806258No Hit
AGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATAGATCGGAAGAG886160.15319850369028495No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT883530.15274383177471051No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAGA874840.15124151278370598No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGAT859970.14867080123062917No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCG837770.14483288620182588No Hit
GTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA827970.1431386714593812No Hit
TTAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG824520.14254223871841853No Hit
GCCGCCTGGATACCGCAGCTCTGTAGGCACCATCAATAGATCGGAAGAGC809510.1399473240975925No Hit
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT799970.1382980579095392Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG797900.1379401982649616No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT789850.13654852186938202No Hit
AGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC779020.1346762416999253No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT775620.1340884529117302No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA772270.13350930807630265No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGATC768810.13291114654478647No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATC763060.13191709197651535No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT762220.1317718735700201No Hit
AAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA757290.1309195798271372Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA740690.1280497875083023No Hit
TGCCGCCTGGATACCGCAGCTCTGTAGGCACCATCAATAGATCGGAAGAG728210.12589225689751557No Hit
GCCGCCTGGATACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC699150.120868391549001No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC697920.12065575031091864Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC689770.11924678600980394No Hit
TTTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA685580.11852242276788115No Hit
AGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGA660760.11423156461405692No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC658500.11384085794896254No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG655360.11329801771515884No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGATCG650780.11250623164164895No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA650420.11244399518172243No Hit
TCGACGAAGCCGAGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA649740.1123264374240834No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA647280.11190115494791872No Hit
CCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC646640.11179051235249376No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA638620.11040402232857473No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA635760.10990958823026944No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG629300.10879278953269875No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC628470.108649299916757No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA623430.10777798947778545No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC619920.10717118399350169No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG615320.10637594033888478No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT613070.10598696246434391No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC602600.1041769187548137No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCA594930.10285093640026771No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCG586210.10134343103760263No Hit
TGGGGTGGGGGCCGGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCA585720.10125872030048039No Hit
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG583340.10084726814874381Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)