FastQCFastQC Report
Wed 6 Sept 2023
SRR13794644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13794644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54343423
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA36526296.721381904853509Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA15724742.8935865891259738No Hit
TGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG10558381.942899327486235No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7773121.4303699639972993No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA6655171.2246504972644068No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG4585190.8437433173835958No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA4367020.8035967848399981No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT3925790.7224038868512203No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3807080.7005594770870432No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3601050.6626468855301956No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG3520450.6478152839212944No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC3510760.6460321794598769No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG3146810.5790599535844476No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA2997590.5516012489680674No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG2964020.5454238684964692No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA2759020.5077008122951696No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT2737480.5037371311704086No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA2496680.45942634125200393No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2394040.4405390510641922No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG2374380.43692131796703343No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2335530.42977233877961646No Hit
AACCTCGGGCCGATCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2313960.4258031372076065No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC2271350.41796226196498515No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA2232490.4108114426284852No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2187160.4024700468352904No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2034060.37429736437471006No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1931100.35535118941624266No Hit
GTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA1866980.34355215349610935No Hit
TTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1792370.3298228011879193No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1765150.3248139153840199No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1742960.3207306245688646No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA1742680.32067910039454084No Hit
TTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1700230.3128676675372473No Hit
AACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCA1695230.3119475929957522No Hit
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1672340.3077354917447876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1642130.3021764013650741No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT1636160.30107783236252894No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG1620140.2981299135315786No Hit
GCCGCCTGGATACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1618480.2978244487838022No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATCG1551780.2855506544002574No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1505150.276970039226274No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT1468740.2702700564151066No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1430450.263224125576337Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGCCTGGATACCGCAGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1385560.25496369634279387No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGATCGG1381620.2542386776040957No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1367680.2516735097824073No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCGG1263030.23241634962891464No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1236700.22757123709340135No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1225030.22542378311355177No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG1180540.21723695984332825No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAGA1167950.21492021214784351No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGATCGGA1165670.21450065815692176No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1153010.21217102941785615No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1118120.20575074926730322No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATCGG1110750.2043945593931394No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA1083200.19932494866950137No Hit
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1070620.19701004112309967Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAG1059410.19494723400106762No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCG1057680.1946288882097103No Hit
TTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1005820.18508587506532298No Hit
CCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT995880.1832567668768307No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGATC978300.18002178478893388No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA972690.17898946115337638No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA965310.17763143113012958No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCCTGTAGGCACCATCAATAGATC958420.17636356841194933No Hit
TGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG904140.16637523918947836No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG895240.16473750650561708No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATCG855930.1575038804603825No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT845000.15549259751267416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT841240.15480070145746982No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG825960.15198895365866077No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG803480.14785229852009873No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA797480.14674820907030459No Hit
AGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATAGATCGGAAGAG794200.1461446401710838No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA783390.14415543901237138No Hit
ACCGCAGCTAGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG773160.1422729665004724No Hit
TCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC732680.13482404301252796No Hit
CTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG711550.13093580800016957No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA705230.12977283377971977No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA703480.12945080769019648No Hit
CCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCGG702450.12926127233464849No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATA696500.1281663836302693No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG693030.12752785189847168No Hit
TCAACCCGGCGGCGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA671540.12357337151912569No Hit
GCCGCCTGGATACCGCAGCTAGGAATCTGTAGGCACCATCAATAGATCGG668950.12309677290663122No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCTGTAGGCACCATCAATAGATCG660800.1215970514039942No Hit
GCGGAGAGCCGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT652740.12011389124310407No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT650710.11974034097925704No Hit
GGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC632640.11641519158629371No Hit
CCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATAGATCGGA632480.11638574920096587No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGAT627480.11546567465947075No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA627290.11543071182689393No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG625790.1151546894644454No Hit
AATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC596370.10974097086228816No Hit
ACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC587500.10810875862567583No Hit
AACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCAC577890.10634037535692221No Hit
GTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGATCGGAAGAG575170.10583985480634887No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA572870.10541662051726111No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA570510.10498234533367543No Hit
TCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT559960.10304098805112075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA548520.10093585750017992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)